Anti-Inflammatory Effects of The Chloroform Extract of Annona muricata Leaves on Phospholipase A2 and Prostaglandin Synthase Activities

Ar, Oyekachukwu; Jp, Elijah; Ov, Eshu; Nwodo, Ofc

doi:10.21767/2172-0479.100137

Cited by 4 publications

(3 citation statements)

References 21 publications

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“…With doses of 0.2-0.6 mg/mL, the enzyme activity was inhibited by 23.91%-43.48% [185]. In the same study, the chloroform extract of A. muricata leaves at 0.5 and 1.0 mg/mL also inhibited prostaglandin synthase activity by 87.46% and 82.92%, respectively, compared to the positive control indomethacin at 1 mg/mL which reduced enzyme activity by 87.46% [185].…”

Section: Anti-inflammatory Activitymentioning

confidence: 99%

“…The anti-inflammatory effects of Annona plants have been reported in many studies; for instance, after one day of orally administered doses of 200 and 400 mg/kg of A. squamosa root extract in an acute carrageenan-induced rat paw edema model, significant inhibition was produced with 24% and 47% inhibition respectively compared to diclofenac sodium inhibiting inflammation by 72% [184]. In an in vitro study, the chloroform extract of A. muricata leaves significantly inhibited activity of phospholipase A 2 [185]. With doses of 0.2-0.6 mg/mL, the enzyme activity was inhibited by 23.91%-43.48% [185].…”

Section: Anti-inflammatory Activitymentioning

confidence: 99%

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Traditional Uses, Phytochemistry and Pharmacological Activities of Annona Genus

Kazman¹,

Hanrahan²

2022

Preprint

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In 1789, the Annonaceae family was cataloged by de Jussieu. It encompasses tropical and subtropical plants which are widespread in distribution across various continents such as Asia, South and Central America, Australia and Africa. The genus of Annona is one of the 120 genera of the Annonaceae family and contains more than 119 species of trees and shrubs. The majority of these species are found in tropical America with more than 105 species (26 of them are endemic). Due to its edible fruits and medicinal properties, Annona is the most important genus of Annonaceae family. Despite Annona having many species, only limited species of this family are economically important such as A. squamosa L. (sugar apple), A. cherimola Mill. (Cherimoya), A. muricata L. (guanabana or soursop), A. atemoya Mabb. (atemoya), a hybrid between A. cherimola and A. squamosa, A. reticulata L. (custard apple), A. glabra L. (pond-apple) and A. macroprophyllata Donn. Sm. (ilama). Phytochemically, several classes of secondary metabolites such as acetogenins, essential oils, alkaloids, terpenoids and flavonoids have been described in this genus. A variety of pharmacological activities have been reported from various parts of Annona species specially leaves and seeds including applications against antibacterial, anticancer, antidiabetic and anti-inflammatory.

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Section: Anti-inflammatory Activitymentioning

confidence: 99%

Section: Anti-inflammatory Activitymentioning

confidence: 99%

Traditional Uses, Phytochemistry and Pharmacological Activities of Annona Genus

Kazman¹,

Hanrahan²

2022

Preprint

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“…Leukocytes accumulate following a series of the process involving migration, attachment, migration, phagocytosis and finally the extracellular release of leukocyte products [2]. Standard anti-inflammatory drugs, e.g., corticoids and NSAIDS, are used to alleviate inflammation by inhibiting the phospholipase A2 (PLA2) and cyclooxygenase (COX), respectively [3]. Prostacyclin and prostaglandin E2 are two mediators responsible for inflammatory symptoms and protection of gastric mucosa, and alleviate inflammation by inhibiting these two mediators, resulting in vomiting, nausea, gastric bleeding, and peptic ulcers [4].…”

Section: Introductionmentioning

confidence: 99%

In Vivo and In Vitro Evaluation of Preventive Activity of Inflammation and Free Radical Scavenging Potential of Plant Extracts from Oldenlandia corymbosa L.

et al. 2021

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Aims: The present study evaluates the anti-inflammatory and antioxidant activity of the crude dichloromethane (CDCME), ethyl acetate (CEAE), and methanol (CMeE) extracts from the plant Oldenlandia corymbosa L. Background:Oldenlandia species have been popular among the people of the Indian subcontinent to treat several types of internal and external inflammation for a long time. Plant decoctions have been used to battle inflammation in cases of tonsilitis, pneumonia and cholecystitis, among others. Objective: The present work designed to demonstrate the properties of the previously mentioned plant extracts to prevent inflammation both in vivo and in vitro. This work is the first investigation of such extracts from this species and their relationship with anti-inflammatory activity. Method: The anti-inflammatory properties of the Oldenlandia corymbosa L. extracts were evaluated in vitro with the Red Blood Cell (RBC) membrane stabilization method and the protein denaturation method and in vivo with the carrageenan-induced paw oedema method. Furthermore, the free radical scavenging activity of the extracts was carried out with the 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical oxidation, total antioxidant capacity and iron reduction assay. Result: Both in vivo and in vitro studies showed that CDCME had the most predominant effects with the value of 80.5% for RBC membrane stabilization, 60% for inhibition of protein denaturation at the concentration of 1000 µg/mL and 63.28% (after 3 h, * p < 0.05) for inhibition of paw oedema (300 mg/kg bwt) compared to carrageenan-induced mice. The free radical scavenging activity was studied by DPPH, total antioxidant and reducing activity assay. CDCME showed scavenging activity in all the methods and an IC50 value of 473.86 µg/mL for DPPH method. Conclusions: The findings of the study remarked that CDCME of the plant has strong anti-inflammatory and antioxidant effects that validate the traditional use of the plant to get remedy from pain. Other: The plants Oldenlandiacorymbosa Linn were provided by the Bangladesh Council of Scientific and Industrial Research Laboratory campus, Rajshahi, Bangladesh. Experiments on animals were conducted by ethical permission of Institute of Biological Sciences, University of Rajshahi, Bangladesh (license no: 225/320-IAMEBBC/IBSc).

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Studies on In-vitro Anti-inflammatory and Antioxidant Potentials of Annona muricata Leaf Extracts

Nwaehujor

Olatunji

Atolanı

et al. 2020

Croat. j. food sci. technol. (Online)

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Inflammation has stimulated significant worldwide scientific interest because of its implication in many human diseases. Most inflammations are caused by reactive oxygen species or free radicals. Annona muricataleaf extracts were investigated for their in-vitroantioxidant and anti-inflammatory potentials. Annona muricataleavesweredried at room temperature, blended using a mill.and extracted with solvents of varying degree of polarities. The solventsused were hexane, ethyl acetate,and ethanol. After sequential extraction, the crude extracts were examined for their in-vitroanti-inflammatory activities on lipoxygenase inhibition, proteinase inhibition, albumin denaturation inhibition,and red blood cell membrane stabilization assays,while the antioxidant activities were examined using DPPH, ABTS and hydrogen peroxide assays. The results showed that the ethanol extract had significantlyhigher albumin denaturation inhibition activity at 500 μg/mL (p < 0.01). The activity of all the extracts on proteinase inhibition decreased with the increase in concentration of the extracts. Indomethacin (standard), ethanol extract,and ethyl acetate extract exhibited a dose dependent increase in lipoxygenase activity. The ethanol extract showed highred blood cell membrane stabilization activity at 500 μg/mL and the activity was comparable with that of the standard (diclofenac). Hydrogen peroxide scavenging activity of the extracts and standard (Vitamin C) were comparable at 20 –100 μg/mL. The ethanol extract showed significantly higher(p < 0.01) DPPH radical scavenging activity compared with other extracts. A similar trend was also observed for ABTS radical scavenging activity. Generally,the ethanol extract exhibited higher anti-inflammatory and antioxidant activities in most of the assays, this could be attributed to the polar compounds present in the extract.

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Anti-Inflammatory Effects of The Chloroform Extract of Annona muricata Leaves on Phospholipase A2 and Prostaglandin Synthase Activities

Cited by 4 publications

References 21 publications

Traditional Uses, Phytochemistry and Pharmacological Activities of Annona Genus

Traditional Uses, Phytochemistry and Pharmacological Activities of Annona Genus

In Vivo and In Vitro Evaluation of Preventive Activity of Inflammation and Free Radical Scavenging Potential of Plant Extracts from Oldenlandia corymbosa L.

Studies on In-vitro Anti-inflammatory and Antioxidant Potentials of Annona muricata Leaf Extracts

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