Anti-inflammatory effects of resveratrol occur via inhibition of lipopolysaccharide-induced NF-κB activation in Caco-2 and SW480 human colon cancer cells

Abstract

Resveratrol, a polyphenol abundantly found in grapes and red wine, exhibits beneficial health effects due to its anti-inflammatory properties. In the present study, we evaluated the effect of resveratrol on inflammatory responses induced by lipopolysaccharide (LPS) treatment of human intestinal Caco-2 and SW480 cell lines. In the LPS-treated intestinal cells, resveratrol dose-dependently inhibited the expression of inducible NO synthase (iNOS) mRNA as well as protein expression, resulting in a decreased production of NO. In addition, Toll-like receptor-4 expression was significantly diminished in LPS-stimulated cells after resveratrol pre-treatment. To investigate the mechanisms by which resveratrol reduces NO production and iNOS expression, we examined the activation of inhibitor of kB (IkB) in LPS-stimulated intestinal cells. Results demonstrated that resveratrol inhibited the phosphorylation, as well as the degradation, of the IkB complex. Overall, these results show that resveratrol is able to reduce LPS-induced inflammatory responses by intestinal cells, interfering with the activation of NF-kB-dependent molecular mechanisms.

Anti-tumor effects and cellular mechanisms of resveratrol

“…Though the detailed mechanisms still need to be studied, it can be supposed that resveratrol inhibits NF-κB through a direct action on the nuclear transcription factor via phosphorylation inhibition, or by TLR-4 down-regulation (84).…”

Toll-Like Receptor-4 Modulation for Cancer Immunotherapy

“…More studies are warranted to understand the mechanism of SPA4 peptide activity. Other agents, including resveratrol (64), NI-0101 antibody (65), and paeoniflorin (66), have also shown suppression of inflammation-induced carcinogenesis.…”

Wine consumption and intestinal redox homeostasis

“…Its antiproliferative activity has been tested in different intestinal cancer cells [60]. In CaCo-2 and SW480 human colon cancer cells resveratrol has been shown to inhibit lipopolysaccharide (LPS)-induced NF-κB activation [61], to induce cell–cycle arrest and apoptosis, and to decrease collagen synthesis in primary rat intestinal smooth muscle cells isolated from colon muscularis externa [62].…”