Anti‐Inflammatory Effects of Pomegranate Peel Extract in THP‐1 Cells Exposed to Particulate Matter PM10

Park, Soojin; Seok, Jin Kyung; Kwak, Jun Yup; Suh, Hwa-Jin; Kim, Young Mi; Boo, Yong Chool

doi:10.1155/2016/6836080

Cited by 36 publications

(32 citation statements)

References 29 publications

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“…An extract of Eucheuma cottonii reduces both the accumulation of alveolar macrophages and the serum levels of malondialdehyde in PM10 coal dust-exposed rats, indicating that the extract attenuates inflammation and oxidative stress resulting from chronic coal dust exposure [26]. We previously demonstrated that pomegranate peel extract and punicalagin attenuate PM10-induced ROS production, the expression of TNF-α, IL-1β, monocyte chemoattractant protein-1, and intercellular adhesion molecule-1 in THP-1 human acute monocytic leukemia cells, indicating their anti-inflammatory activities against particulate matter-induced inflammation [20]. However, the efficacy of the plant extracts or their constituents on skin cells exposed to air pollution remain unexplored.…”

Section: Discussionmentioning

confidence: 99%

“…We previously reported that pomegranate peel extract attenuates the oxidative and inflammatory responses of monocytes exposed to PM10 [20]. However, the effects of polyphenols on PM10-exposed skin cells remain unexplored.…”

Section: Introductionmentioning

confidence: 99%

“…However, the effects of polyphenols on PM10-exposed skin cells remain unexplored. Punicalagin and (–)-epigallocatechin-3-gallate (EGCG) are natural polyphenols contained in pomegranate ( Punica granatum L.) [20] and green tea ( Camellia sinensis L.) [17], respectively; they are report ed to exhibit diverse bioactivities [21]. In the present study, we examined their effects on viability, ROS production, and the expression of proinflammatory cytokines and MMP-1 in human epidermal keratinocytes exposed to PM10.…”

Section: Introductionmentioning

confidence: 99%

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Punicalagin and (–)-Epigallocatechin-3-Gallate Rescue Cell Viability and Attenuate Inflammatory Responses of Human Epidermal Keratinocytes Exposed to Airborne Particulate Matter PM10

Seok

Lee

Kim³

et al. 2018

Skin Pharmacol Physiol

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Background/Aims: Airborne particulate matter with a diameter of < 10 µm (PM10) causes oxidative damage, inflammation, and premature skin aging. In this study, we evaluated whether polyphenolic antioxidants attenuate the inflammatory responses of PM10-exposed keratinocytes. Methods: Primary human epidermal keratinocytes were exposed in vitro to PM10 in the absence or presence of punicalagin and (–)-epigallocatechin-3-gallate (EGCG), which are the major polyphenolic antioxidants found in pomegranate and green tea, respectively. Assays were performed to determine cell viability, production of reactive oxygen species (ROS), and expression of NADPH oxidases (NOX), proinflammatory cytokines, and matrix metalloproteinase (MMP)-1. Results: PM10 decreased cell viability and increased ROS production in a dose-dependent manner. It also increased the expression levels of NOX-1, NOX-2, tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, IL-8, and MMP-1. Punicalagin was not cytotoxic up to 300 μM, and (–)-EGCG was cytotoxic above 30 μM, respectively. Further, punicalagin (3–30 μM) and EGCG (3–10 μM) rescued the viability of PM10-exposed cells. They also attenuated ROS production and the expression of NOX-1, NOX-2, TNF-α, IL-1β, IL-6, IL-8, and MMP-1 stimulated by PM10. Conclusions: This study demonstrates that polyphenolic antioxidants, such as punicalagin and (–)-EGCG, rescue keratinocyte viability and attenuate the inflammatory responses of these cells due to airborne particles.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Punicalagin and (–)-Epigallocatechin-3-Gallate Rescue Cell Viability and Attenuate Inflammatory Responses of Human Epidermal Keratinocytes Exposed to Airborne Particulate Matter PM10

Seok

Lee

Kim³

et al. 2018

Skin Pharmacol Physiol

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“…Pomegranate extracts have broad bioactivities including antimicrobial, antiinflammatory and anti‐tumor effects (Betanzos‐Cabrera et al, ; Ma et al, ; Park et al, ) and have been used in traditional Uyghur medicine for thousands of years. Our results show that GA decreased membrane CYP51 enzyme activity and increased fungal lanosterol in a dose‐dependent manner.…”

Section: Discussionmentioning

confidence: 99%

Antifungal Activity of Gallic Acid In Vitro and In Vivo

Dawuti³

et al. 2017

Phytotherapy Research

145

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Gallic acid (GA) is a polyphenol natural compound found in many medicinal plant species, including pomegranate rind (Punica granatum L.), and has been shown to have antiinflammatory and antibacterial properties. Pomegranate rind is used to treat bacterial and fungal pathogens in Uyghur and other systems of traditional medicine, but, surprisingly, the effects of GA on antifungal activity have not yet been reported. In this study, we aimed to investigate the inhibitory effects of GA on fungal strains both in vitro and in vivo. The minimal inhibitory concentration (MIC) was determined by the NCCLS (M38-A and M27-A2) standard method in vitro, and GA was found to have a broad spectrum of antifungal activity, with MICs for all the tested dermatophyte strains between 43.75 and 83.33 μg/mL. Gallic acid was also active against three Candida strains, with MICs between 12.5 and 100.0 μg/mL. The most sensitive Candida species was Candida albicans (MIC = 12.5 μg/mL), and the most sensitive filamentous species was Trichophyton rubrum (MIC = 43.75 μg/mL), which was comparable in potency to the control, fluconazole. The mechanism of action was investigated for inhibition of ergosterol biosynthesis using an HPLC-based assay and an enzyme linked immunosorbent assay. Gallic acid reduced the activity of sterol 14α-demethylase P450 (CYP51) and squalene epoxidase in the T. rubrum membrane, respectively. In vivo model demonstrated that intraperitoneal injection administration of GA (80 mg/kg d) significantly enhanced the cure rate in a mice infection model of systemic fungal infection. Overall, our results confirm the antifungal effects of GA and suggest a mechanism of action, suggesting that GA has the potential to be developed further as a natural antifungal agent for clinical use. Copyright © 2017 John Wiley & Sons, Ltd.

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“…The extract of Eucheuma cottonii attenuated inflammation and oxidative stress induced by chronic exposure to coal dust in rats [ 10 ]. Pomegranate peel extract and punicalagin attenuated the PM10-induced adhesion of THP-1 cells to endothelial cells, which was indicative of its anti-inflammatory activity against particulate matter [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Ecklonia cava Extract and Dieckol Attenuate Cellular Lipid Peroxidation in Keratinocytes Exposed to PM10

Lee

Seok

Boo

2018

Evidence-Based Complementary and Alternative Medicine

Self Cite

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Airborne particulate matter can cause oxidative stress, inflammation, and premature skin aging. Marine plants such as Ecklonia cava Kjellman contain high amounts of polyphenolic antioxidants. The purpose of this study was to examine the antioxidative effects of E. cava extract in cultured keratinocytes exposed to airborne particulate matter with a diameter of <10 μm (PM10). After the exposure of cultured HaCaT keratinocytes to PM10 in the absence and presence of E. cava extract and its constituents, cell viability and cellular lipid peroxidation were assessed. The effects of eckol and dieckol on cellular lipid peroxidation and cytokine expression were examined in human epidermal keratinocytes exposed to PM10. The total phenolic content of E. cava extract was the highest among the 50 marine plant extracts examined. The exposure of HaCaT cells to PM10 decreased cell viability and increased lipid peroxidation. The PM10-induced cellular lipid peroxidation was attenuated by E. cava extract and its ethyl acetate fraction. Dieckol more effectively attenuated cellular lipid peroxidation than eckol in both HaCaT cells and human epidermal keratinocytes. Dieckol and eckol attenuated the expression of inflammatory cytokines such as tumor necrosis factor- (TNF-) α, interleukin- (IL-) 1β, IL-6, and IL-8 in human epidermal keratinocytes stimulated with PM10. This study suggested that the polyphenolic constituents of E. cava, such as dieckol, attenuated the oxidative and inflammatory reactions in skin cells exposed to airborne particulate matter.

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Anti‐Inflammatory Effects of Pomegranate Peel Extract in THP‐1 Cells Exposed to Particulate Matter PM10

Cited by 36 publications

References 29 publications

Punicalagin and (–)-Epigallocatechin-3-Gallate Rescue Cell Viability and Attenuate Inflammatory Responses of Human Epidermal Keratinocytes Exposed to Airborne Particulate Matter PM10

Punicalagin and (–)-Epigallocatechin-3-Gallate Rescue Cell Viability and Attenuate Inflammatory Responses of Human Epidermal Keratinocytes Exposed to Airborne Particulate Matter PM10

Antifungal Activity of Gallic Acid In Vitro and In Vivo

Ecklonia cava Extract and Dieckol Attenuate Cellular Lipid Peroxidation in Keratinocytes Exposed to PM10

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