Anti-Inflammatory Effects of Metabolites from Antarctic Fungal Strain Pleosporales sp. SF-7343 in HaCaT Human Keratinocytes

Dong, Linsha; Kim, Hye Jin; Cao, Thao Quyen; Liu, Zhiming; Lee, Hwan; Ko, Wonmin; Kim, Youn‐Chul; Sohn, Jae Hak; Kim, Tai Kyoung; Yim, Joung Han; Lee, Dong-Sung; Oh, Hyuncheol

doi:10.3390/ijms22189674

Cited by 12 publications

(10 citation statements)

References 37 publications

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“…A previous study demonstrated that the HO-1 level in human osteoarthritis (OA) cartilages from type 2 diabetes mellitus (T2DM) after CoPP treatment was significantly lower than those from non-T2DM (Vaamonde-Garcia et al 2017 ). Given the contrasting effect of CoPP seen in HaCaT cultured in NG media versus those cultured in HG media, it was decided to investigated whether the difference in glucose concentration had any effect on the protein expressions of HO-1 and its upstream regulator, nuclear factor erythroid 2-related factor 2 (Nrf2) after CoPP treatment (Dong et al 2021 ). At the end of the migration assays, cells from each treatment group were collected and subjected to Western blotting analysis (Fig.…”

Section: Resultsmentioning

confidence: 99%

Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions

Fang

Lai²,

Chen³

et al. 2022

Mol Med

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Background Complete healing of diabetic wounds continues to be a clinically unmet need. Although robust therapies such as stem cell therapy and growth factor treatment are clinically applied, these treatments are costly for most diabetic wound patients. Therefore, a cheaper alternative is needed. Cobalt protoporphyrin (CoPP) has recently been demonstrated to promote tissue regeneration. In this study, the therapeutic benefits of CoPP in diabetic wound healing were examined. Methods An in vitro wound healing model that mimics re-epithelialization was established to examine the effect of CoPP on the migratory capability of human keratinocytes (HaCaT) in either normal glucose (NG) or high glucose (HG) media, as well as in the presence of either H2O2 or lipopolysaccharide (LPS). At the end of the migration assays, cells were collected and subjected to Western blotting analysis and immunostaining. Results HaCaT were found to migrate significantly more slowly in the HG media compared to the NG media. CoPP treatment was found to enhance cell migration in HG media, but was found to decrease cell migration and proliferation when HaCaT were cultured in NG media. CoPP treatment induced high levels of expression of Nrf-2/HO-1 and FoxO1 in HaCaT cultured in either glucose concentration, although the FoxO1 expression was found to be significantly higher in HaCaT that underwent the migration assay in NG media compared to those in HG media. The higher level of FoxO1 expression seen in CoPP-treated HaCaT cultured in NG media resulted in upregulation of CCL20 and downregulation of TGFβ1. In contrast, HaCaT migrated in HG media were found to have high levels of expression of TGFβ1, and low levels of expression of CCL20. Interestingly, in the presence of H2O2, CoPP-pretreated HaCaT cultured in either NG or HG media had similar expression level of Nrf-2/HO-1 and FoxO1 to each other. Moreover, the anti-apoptotic effect of CoPP pretreatment was noticed in HaCaT cultured in either glucose concentration. Additionally, CoPP pretreatment was shown to promote tight junction formation in HaCaT suffering from LPS-induced damage. Conclusions CoPP enhances cell migratory capacity under hyperglycemic conditions, and protects cells from oxidative and LPS-induced cellular damage in HG media containing either H2O2 or LPS.

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Section: Resultsmentioning

confidence: 99%

Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions

Fang

Lai²,

Chen³

et al. 2022

Mol Med

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“…HaCaT cells were cultured in DMEM media added with 10%FBS. For the source of antibody and other reagent, refer to our published articles [ 34 ].…”

Section: Methodsmentioning

confidence: 99%

“…HaCaT cells were seeded at a density of 2 × 10 4 cells in a 48-well plate for 24 h and then treated with compounds 1 – 4 (20–80 μM). The determination method of MTT assay is performed according to the previous paper [ 34 ].…”

Section: Methodsmentioning

confidence: 99%

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Anti-Inflammatory Effects Exerted by 14-Methoxyalternate C from Antarctic Fungal Strain Pleosporales sp. SF-7343 via the Regulation of NF-κB and JAK2/STAT3 in HaCaT Human Keratinocytes

Dong

Cao

Liu

et al. 2022

IJMS

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Atopic dermatitis (AD) is a chronic inflammatory skin disease with a profound negative impact on patients’ quality of life. Four known secondary fungal metabolites were found in the chemical study of the Antarctic fungus Pleosporales sp. SF-7343, including 14-methoxyalternate C (1), 5′-methoxy-6-methyl-biphenyl-3,4,3′-triol (2), 3,8,10-trihydroxy-4-methoxy-6-methylbenzocoumarin (3), and alternariol monomethyl ether (4). Additionally, we identified the skin anti-inflammatory composition from the SF-7343 strain. Interleukin-8 and -6 Screening results showed that compound 1 inhibited IL-8 and IL-6 in tumor necrosis factor-α/interferon-γ stimulated HaCaT cells. Compound 1 showed inhibitory effects on MDC and RANTES. It also downregulated the expression of intercellular adhesion molecule-1 (ICAM-1) and upregulated the expression of involucrin. The results of the mechanistic study showed that compound 1 inhibited the nuclear translocation of nuclear factor-kappa B p65 and STAT3. In conclusion, this study demonstrates the potential of the Antarctic fungal strain SF-7343 as a bioactive resource to inhibit skin inflammation, such as AD.

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“…Previous chemical investigations of Pleosporales sp. led to the isolation of heptaketides, chlamydosporols, diterpenoids, azaphilones, phenalenones, and other compounds. − As a part of our ongoing study to discover novel bioactive metabolites from fungal endophytes, Pleosporales sp. YY-4 derived from the medicinal plant Uncaria rhynchophylla (Miq.)…”

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confidence: 99%

Anti-inflammatory Dimeric Benzophenones from an Endophytic Pleosporales Species

Zeng

et al. 2022

J. Nat. Prod.

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Eight new polyketides, including three dimeric benzophenones, named dipleosporones A−C (1−3), three benzophenones (4−6), one xanthone (7), and one phenylbenzoate (8), along with seven known polyketides (9−15) were isolated from the fungus Pleosporales sp. YY-4. The structures of the new compounds were established on the basis of spectroscopic methods, including high-resolution electrospray ionization mass spectrometry and one-and two-dimensional nuclear magnetic resonance. This is the first report of a benzophenone dimer connection via a C bridge from natural sources. An antiinflammatory assay indicated that the dimeric benzophenones (1−3) inhibited lipopolysaccharide-induced NO production in RAW 264.7 cells, with half-maximal inhibitory concentration (IC 50 ) values ranging from 8.8 to 18.1 μM, being more potent than the positive control, dexamethasone (IC 50 = 22.2 μM).

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Anti-Inflammatory Effects of Metabolites from Antarctic Fungal Strain Pleosporales sp. SF-7343 in HaCaT Human Keratinocytes

Cited by 12 publications

References 37 publications

Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions

Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions

Anti-Inflammatory Effects Exerted by 14-Methoxyalternate C from Antarctic Fungal Strain Pleosporales sp. SF-7343 via the Regulation of NF-κB and JAK2/STAT3 in HaCaT Human Keratinocytes

Anti-inflammatory Dimeric Benzophenones from an Endophytic Pleosporales Species

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