Anti-Inflammatory Effects of M-MSCs in DNCB-Induced Atopic Dermatitis Mice

Ryu, Bokyeong; Baek, Jeong‐In; Kim, Hana; Lee, Ji-Heon; Kim, Jin; Jeong, Young-Hoon; Lee, Seulgi; Kang, Kitaek; Oh, Minseok; Kim, Eun Young; Kim, C‐Yoon; Chung, Hyung Min

doi:10.3390/biomedicines8100439

Cited by 11 publications

(16 citation statements)

References 42 publications

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“…MSC-derived exosomes have the potential for therapeutic application in wound healing via tissue regeneration and regulation of immune response and inflammation [ 42 ]. It has been reported that secretion of MSCs with anti-inflammatory properties has beneficial effects on atopic dermatitis-related inflammatory lesions, such as pruritus [ 43 ], but the effects of exosomes on itching have not been studied. Previous studies have demonstrated that exosomes derived from MSCs could accelerate normal and diabetic wound healing [ 2 , 44 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

SHED-derived exosomes promote LPS-induced wound healing with less itching by stimulating macrophage autophagy

Xie

Cheng

et al. 2022

J Nanobiotechnol

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High-quality cutaneous wound healing is associated with rapid wound closure and a comfortable healing process. Currently, exosomes derived from mesenchymal stem cells displayed a prominent therapeutic effect on skin wound closure. But the therapeutic approaches for wound itching are very limited in clinical. Stem cells from human exfoliated deciduous teeth (SHED) may offer a unique exosome resource for cell-free therapeutics in potential clinical applications. Here, we investigated the common mechanisms underlying wound closure and unpleasant sensation of itching, focusing on the contribution of the SHED-derived exosome to immune response and wound itching during healing. The effects of SHED-derived exosomes on inflammatory wound healing were examined using lipopolysaccharide (LPS)-induced wounds in a mouse model. We found prolonged inflammation and distinct itch responses in skin wound tissue during LPS-induced wound healing. SHED-derived exosomes facilitated LPS-induced wound closure and relieved wound itching. Therefore, they are ideal for the treatment of wound healing. Macrophages in skin wound tissues are responsible for autophagy during wound healing. Macrophage autophagy also regulates cell proliferation, migration, and neuronal signal transduction in vitro. SHED-derived exosomes containing miR-1246 enhanced autophagy by regulating macrophage function through the AKT, ERK1/2, and STAT3 signaling pathways. Thus, SHED-derived exosomes promote wound healing with less itching in an LPS-induced wound model by stimulating macrophage autophagy, which has implications for the treatment of inflammatory wound healing. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

SHED-derived exosomes promote LPS-induced wound healing with less itching by stimulating macrophage autophagy

Xie

Cheng

et al. 2022

J Nanobiotechnol

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“…Secretion was confirmed. [ 13 ] Based on these results, it was expected that M-MSCs would have the ability to control inflammation through the paracrine effect, so cells were injected into the muscle instead of the completely amputated tendon. To demonstrate these characteristics of the M-MSCs in transcriptomic level, the cells were compared with BM-MSCs and hDFs.…”

Section: Discussionmentioning

confidence: 99%

“…The M-MSCs were obtained from embryoid bodies (EBs) of hPSCs as previously described [ 12 , 13 ]. Briefly, SNUhES cells between passage 40–60 were cultured in DMEM/F-12 Medium supplemented with 20% knockout serum replacement, 1 mM glutamine, 0.1 mM β-mercaptoethanol, 0.1 mM nonessential amino acids, and 4 ng/mL human recombinant bFGF, all of which were purchased from Invitrogen Corporation (Carlsbad, CA, USA) (hPSC culture media).…”

Section: Methodsmentioning

confidence: 99%

Immunomodulation of Pluripotent Stem Cell-Derived Mesenchymal Stem Cells in Rotator Cuff Tears Model

et al. 2022

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Background: Rotator cuff tears (RCTs) induce chronic muscle weakness and shoulder pain. Treatment of RCT using surgery or drugs causes lipid infiltration and fibrosis, which hampers tissue regeneration and complete recovery. The pluripotent stem cell-derived multipotent mesenchymal stem cells (M-MSCs) represent potential candidate next-generation therapies for RCT. Methods: The difference between M-MSCs and adult-MSCs was compared and analyzed using next-generation sequencing (NGS). In addition, using a rat model of RCT, the muscle recovery ability of M-MSCs and adult-MSCs was evaluated by conducting a histological analysis and monitoring the cytokine expression level. Results: Using NGS, it was confirmed that M-MSC was suitable for transplantation because of its excellent ability to regulate inflammation that promotes tissue repair and reduced apoptosis and rejection during transplantation. In addition, while M-MSCs persisted for up to 8 weeks in vivo, they significantly reduced inflammation and adipogenesis-related cytokine levels in rat muscle. Significant differences were also confirmed in histopathological remission. Conclusions: M-MSCs remain in the body longer to modulate immune responses in RCTs and have a greater potential to improve muscle recovery by alleviating acute inflammatory responses. This indicates that M-MSCs could be used in potential next-generation RCT therapies.

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“…In addition to GVHD, an increasing number of studies and approximately a thousand MSC clinical trials ramify to most medical specialties, especially cardiovascular medicine, neurology, autoimmune diseases, and musculoskeletal diseases, highlighting that the application potential of MSCs is far from being exhausted [ 6 ]. Therefore, a review covering MSC therapy for nonischemic dilated cardiomyopathy (NIDCM) [ 7 ] and a research article developing MSCs as the next-generation cell-based therapeutics for atopic dermatitis (AD) [ 8 ] are included in this Special Issue.…”

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confidence: 99%

“…Secretions of hESC-derived multipotent MSCs were used to treat a mouse model of AD, showing the utility of hESC-derived multipotent MSCs as an immunoregulator having a significant relief effect in acute AD confirmed by protein expression and histological analyses. Being natural humoral immune regulators, MSC-based therapeutics may stand superior to conventional therapeutics, such as corticosteroids and antihistamines that are subject to various side effects [ 8 ].…”

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confidence: 99%