SHED-derived exosomes promote LPS-induced wound healing with less itching by stimulating macrophage autophagy

Xie, Yunyi; Yu, Le; Cheng, Zhilan; Peng, Yingying; Cao, Zeyuan; Chen, Beichen; Duan, Yihong; Wang, Yan

doi:10.1186/s12951-022-01446-1

Cited by 26 publications

(22 citation statements)

References 67 publications

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“…Previous studies have suggested the involvement of autophagy in immune regulation (Jiang et al, 2019;Wang et al, 2022b;Xie et al, 2022). The autophagic decomposition of cyclic GMP-AMP synthase could be astricted by TRIM14, thus enhancing congenital immunity (Chen et al, 2016).…”

Section: Involvement Of Ncrna-autophagy Axis In Immune Regulationmentioning

confidence: 97%

Non-coding RNAs: The recently accentuated molecules in the regulation of cell autophagy for ovarian cancer pathogenesis and therapeutic response

Peng

Yan

et al. 2023

Front. Pharmacol.

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Autophagy is a self-recycling and conserved process, in which the senescent cytoplasmic components are degraded in cells and then recycled to maintain homeostatic balance. Emerging evidence has suggested the involvement of autophagy in oncogenesis and progression of various cancers, such as ovarian cancer (OC). Meanwhile, the non-coding RNAs (ncRNAs) frequently regulate the mRNA transcription and other functional signaling pathways in cell autophagy, displaying promising roles in human cancer pathogenesis and therapeutic response. This article mainly reviews the cutting-edge research advances about the interactions between ncRNAs and autophagy in OC. This review not only summarizes the underlying mechanisms of dynamic ncRNA-autophagy association in OC, but also discusses their prognostic implications and therapeutic biomarkers. The aim of this review was to provide a more in-depth knowledge framework exploring the ncRNA-autophagy crosstalk and highlight the promising treatment strategies for OC patients.

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Section: Involvement Of Ncrna-autophagy Axis In Immune Regulationmentioning

confidence: 97%

Non-coding RNAs: The recently accentuated molecules in the regulation of cell autophagy for ovarian cancer pathogenesis and therapeutic response

Peng

Yan

et al. 2023

Front. Pharmacol.

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“…Exosome nanoparticles were purified by several ultracentrifugation and filtration steps according to the previously published procedures. [8] Briefly, SHEDs and human adult dental pulp stem cells (DPSCs) at passages 3-6 were utilized for the exosome isolation. The SHEDs and DPSCs were cultured in a complete medium with the fetal bovine serum for 48 h (with depletion of exosomes by 2 h centrifugation at 100 000g), culture supernatants of SHEDs and DPSCs were collected.…”

Section: Isolation and Characterization Of Shed-exo Bio-nanoparticlesmentioning

confidence: 99%

Young Exosome Bio‐Nanoparticles Restore Aging‐Impaired Tendon Stem/Progenitor Cell Function and Reparative Capacity

Jin¹,

Wang²,

Wu³

et al. 2023

Advanced Materials

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Tendon aging is frequently accompanied by extrinsic inflammation-aging harassment and intrinsic TSPC senescence, resulting in impaired reparative and regenerative capacities. [2] Although the functional decline of senescent TSPCs is accepted well, an efficient and precise method to rejuvenate aged TSPC (AT-SC) function and recover aging-impaired tendon reparative capacity remains to be further explored.Extracellular vesicles (EVs) are membrane-enclosed structures mainly that are classified into two main groups: exosomes and microvesicles. Among them, exosomes range from 40 to 150 nm in diameter and are mainly derived from the endosomal system, [3] whereas microvesicles range from 100 to 1000 nm in diameter and are generated by shedding of the plasma membrane. EVs could facilitate intercellular communication by transferring mRNA, microRNA, proteins, and organelle cargoes into recipient cells; [4] therefore, EVs have been suggested as excellent candidates for therapeutic methods for tissue regeneration and repair. Recently, EVs have been proven to be beneficial components of young blood in extending the life span of mice, suggesting that some specific EVs may possess an anti-senescent function. [5] Aging impairs tendon stem/progenitor cell function and tendon homeostasis, however, effective treatments for aging-induced tendon diseases are lacking. Exosomes are naturally derived nanoparticles that contain bioactive molecules, and therefore, have attracted great interest in tissue engineering and regenerative medicine. In this study, it is shown that young exosomes secreted by stem cells from human exfoliated deciduous teeth (SHED-Exos) possess abundant anti-aging signals. These young bio-nanoparticles can alleviate the aging phenotypes of aged tendon stem/progenitor cells (AT-SCs) and maintain their tenogenic capacity. Mechanistically, SHED-Exos modulate histone methylation and inhibit nuclear factor-κB to reverse AT-SC aging. In a naturally aging mouse model, systemic administration of SHED-Exo bio-nanoparticles retards tendon degeneration. Interestingly, local delivery of SHED-Exos-loaded microspheres confers anti-aging phenotypes, including reduced senescent cells and decreased ectopic bone formation, thereby functionally and structurally rescuing endogenous tendon regeneration and repair capacity in aged rats. Overall, SHED-Exos, as natural bioactive nanoparticles, have promising translational and therapeutic potential for aging-related diseases.

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“…SHEDderived exosomes containing miR-1246 also enhanced autophagy by regulating macrophage function through the AKT, ERK1/2, and STAT3 signaling pathways. Therefore, SHED-derived exosomes promote wound healing with less itching in an LPS-induced wound model by stimulating macrophage autophagy, which has implications for the treatment of inflammatory wound healing [50].…”

Section: Other Therapeutic Effects Of Shedmentioning

confidence: 99%

Human Teeth Is Useful Even after Its SHED! So, Why Discard It?

Bhandary¹,

Chhabra²,

Rao³

et al. 2023

Dentistry

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A few decades ago, if one underwent a knee injury that makes walking painful or had an atrophied kidney, then, he/she was condemned to a life hooked on to machines, or on constant medications. However, in today’s era, teeth can be grown in a Petri dish; heart and liver replacements are possible with no risk of rejection because the organs are made of the patient’s own cells. This is the promise of regenerative medicine and tissue engineering. The entire idea of regenerative medicine is based on the presence of stem cells in the body or the ability to introduce stem cells into the body without causing harm. These can be obtained from a variety of body and dental tissues. Deciduous teeth often discarded as biological waste is proven to possess Stem cells (SHED) that have promising applications in tissue engineering and regenerative medicine. Hence, their contribution toward the field of regenerative medicine and dentistry is immense. This chapter summarizes SHED’s regenerative potentials and therapeutic applications; and also focuses on its potential future scope in regenerative dentistry. Furthermore, procedures involved in SHED-induced therapy, from SHED collection to SHED banking, have also been explained.

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SHED-derived exosomes promote LPS-induced wound healing with less itching by stimulating macrophage autophagy

Cited by 26 publications

References 67 publications

Non-coding RNAs: The recently accentuated molecules in the regulation of cell autophagy for ovarian cancer pathogenesis and therapeutic response

Non-coding RNAs: The recently accentuated molecules in the regulation of cell autophagy for ovarian cancer pathogenesis and therapeutic response

Young Exosome Bio‐Nanoparticles Restore Aging‐Impaired Tendon Stem/Progenitor Cell Function and Reparative Capacity

Human Teeth Is Useful Even after Its SHED! So, Why Discard It?

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