Anti-inflammatory effects of locally applied enzyme-loaded ultradeformable vesicles on an acute cutaneous model

Simões, Sandra; Marques, Cláudia Lima; Cruz, M.E.M.; Martins, M.B.F.

doi:10.1080/02652040802630403

Cited by 4 publications

(4 citation statements)

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“…After the ears were weighed, the inflamed tissues were collected, fixed by 4% formalin, dehydrated, embedded in paraffin and cut into 8 μm sections. Finally, the sections were stained with H&E. [38][39][40] The images were observed under a conventional light microscope (Olympus IX71 inverted microscope).…”

Section: Anti-inflammatory Assays In Micementioning

confidence: 99%

Promotion of the transdermal delivery of protein drugs by <em>N</em>-trimethyl chitosan nanoparticles combined with polypropylene electret

Tu¹,

Wang²,

Lü³

et al. 2016

IJN

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Section: Anti-inflammatory Assays In Micementioning

confidence: 99%

Promotion of the transdermal delivery of protein drugs by <em>N</em>-trimethyl chitosan nanoparticles combined with polypropylene electret

Tu¹,

Wang²,

Lü³

et al. 2016

IJN

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“…9,10) In these studies, skin injury was caused by glucose oxidase as a producer of H 2 O 2 or arachidonic acid. 9,10) Therefore, this is the first report on the effect of topical application of SOD on UVinduced skin damage.…”

Section: )mentioning

confidence: 99%

“…9) On the other hand, Simões et al studied topical application of SOD. 10) In this previous study, mouse ear edema model induced by arachidonic acid was used, and SOD reduced the edema. In the present study, we attempted to utilize iontophoretic delivery of SOD encapsulated in cationic liposomes for prevention of UV-induced skin injury.…”

mentioning

confidence: 99%

Efficient Intradermal Delivery of Superoxide Dismutase Using a Combination of Liposomes and Iontophoresis for Protection against UV-Induced Skin Damage

Kigasawa

Miyashita

Kajimoto

et al. 2012

Biological & Pharmaceutical Bulletin

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Superoxide dismutase (SOD) is a potent antioxidant agent that protects against UV-induced skin damage. However, its high molecular weight is a significant obstacle for efficient delivery into the skin through the stratum corneum and development of antioxidant activity. Recently, we developed a non-invasive transfollicular delivery system for macromolecules using a combination of liposomes and iontophoresis, that represents promising technology for enhancing transdermal administration of charged drugs (IJP, 403, 2011, Kajimoto et al.). In this study, in rats we attempted to apply this system to intradermal delivery of SOD for preventing UV-induced skin injury. SOD encapsulating in cationic liposomes was subjected to anodal iontophoresis. After iontophoretic treatment, the liposomes were diffused widely in the viable skin layer around hair follicles. In contrast, passive diffusion failed to transport liposomes efficiently into the skin. Iontophoretic delivery of liposomes encapsulating SOD caused a marked decrease in the production of oxidative products, such as malondialdehyde, hexanoyl lysine, and 8-hydroxi-2-deoxyguanosine, in UV-irradiated skin. These findings suggested that functional SOD can be delivered into the skin using a combination of iontophoresis and a liposomal system. In conclusion, we succeeded in developing an efficient intradermal SOD delivery system, that would be useful for delivery of other macromolecules. Key words superoxide dismutase; iontophoresis; liposome; transfollicular delivery; antioxidant activityThe skin is exposed constitutively to ultraviolet (UV) rays that cause the production of reactive oxygen species (ROS) in the skin, resulting in diverse skin disorders.1) It is well-known that the classes of UV that reach the ground are UVA and UVB. UVA readily penetrates deep layers of the skin, and causes production of melanin and wrinkling associated with destruction of collagen fiber, resulting in aging of the skin. 1)UVB causes skin inflammation and DNA damage that contribute to the pathogenesis of serious skin disorders, such as skin malignancy.1) ROS are also involved in the development of skin damage, and therefore elimination of ROS is essential for protecting the skin from UV-induced damage.Superoxide dismutase (SOD) may be used to prevent and treat ROS-induced disorders of various tissues as it has potent antioxidant activity.2) Because of its poor pharmacokinetic profile, systemic administration of free SOD does not result in induction of efficient in vivo antioxidant activity.3) In contrast, the skin is an available site for topical application of SOD due to its accessibility. Percutaneous delivery of SOD is therefore considered to be a promising method for prophylaxis or therapy of UV-induced skin damage. However, SOD are highly hydrophilic macromolecules and do not readily penetrate the deeper layers of the skin as the outer layer of the skin, the stratum corneum, functions as a significant barrier prohibiting penetration of drug molecules larger than 500 Da. 4)Iontophoresis...

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“…58,59 SOD-loaded PEGylated liposomes also reduced levels of TNF-α and oxidative species more effectively than free SOD in a rat model of peritonitis established via intraperitoneal injection of lipopolysaccharide (LPS), a proinflammatory bacterial endotoxin. 60 SOD and catalase encapsulated within liposomes reduced skin inflammation in a murine ear edema model, 61 as well as periodontal inflammation in a canine model. 62 Nanoparticle Encapsulated Enzymes to Treat Acute and Chronic Inflammation.…”

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confidence: 99%

Enzymes as Immunotherapeutics

et al. 2017

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Enzymes are attractive as immunotherapeutics because they can catalyze shifts in the local availability of immunostimulatory and immunosuppressive signals. Clinical success of enzyme immunotherapeutics frequently hinges upon achieving sustained biocatalysis over relevant time scales. The time scale and location of biocatalysis are often dictated by the location of the substrate. For example, therapeutic enzymes that convert substrates distributed systemically are typically designed to have a long half-life in circulation, whereas enzymes that convert substrates localized to a specific tissue or cell population can be more effective when designed to accumulate at the target site. This Topical Review surveys approaches to improve enzyme immunotherapeutic efficacy via chemical modification, encapsulation, and immobilization that increases enzyme accumulation at target sites or extends enzyme half-life in circulation. Examples provided illustrate "replacement therapies" to restore deficient enzyme function, as well as "enhancement therapies" that augment native enzyme function via supraphysiologic doses. Existing FDA-approved enzyme immunotherapies are highlighted, followed by discussion of emerging experimental strategies such as those designed to enhance antitumor immunity or resolve inflammation.

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Anti-inflammatory effects of locally applied enzyme-loaded ultradeformable vesicles on an acute cutaneous model

Cited by 4 publications

References 0 publications

Promotion of the transdermal delivery of protein drugs by <em>N</em>-trimethyl chitosan nanoparticles combined with polypropylene electret

Promotion of the transdermal delivery of protein drugs by <em>N</em>-trimethyl chitosan nanoparticles combined with polypropylene electret

Efficient Intradermal Delivery of Superoxide Dismutase Using a Combination of Liposomes and Iontophoresis for Protection against UV-Induced Skin Damage

Enzymes as Immunotherapeutics

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