Anti‐inflammatory effects of glutamine on <scp>LPS</scp>‐stimulated human dental pulp cells correlate with activation of <scp>MKP</scp>‐1 and attenuation of the <scp>MAPK</scp> and NF‐κB pathways

Kim, D.-S.; Shin, Mee-Ran; Kim, Y.-S.; Bae, Won Kyung; Roh, Dae Hyun; Hwang, Young-Hee; Kim, E.-C.

doi:10.1111/iej.12303

Cited by 38 publications

(28 citation statements)

References 32 publications

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“…These data agreed with previous studies indicating occludin function could be regulated in a manner dependent on NF-κB [45, 46]. Meanwhile, it has been suggested that IκBα is associated with MKP-1 dependent negative regulation, which is attributed to modulate the immune response [13, 14]. In a kinetic analysis of IκBα phosphorylation, we found primarily an effect of MKP-1 deficiency on the up-regulation of IκBα activation that led to markedly increased phospho-IκBα levels in Sertoli cells by LPS stimulation.…”

Section: Discussionsupporting

confidence: 92%

MKP-1 attenuates LPS-induced blood-testis barrier dysfunction and inflammatory response through p38 and IκBα pathways

et al. 2016

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Sertoli cells create a local tolerogenic microenvironment to maintain testicular immune privilege especially through the formation of a blood-testis barrier (BTB). However, the molecular mechanisms underlying the immune modulation function and BTB dynamics of Sertoli cells remained elusive. MAP phosphatase (MKP)-1 acts as a crucial negative regulator of the inflammatory response. Nevertheless, the role of MKP-1 in regulating Sertoli cells has not been elucidated. In this study, we have for the first time uncovered distinct cellular localization of MKP-1 in the cells at different stages of mouse testis, and the level of MKP-1 expression was significantly up-regulated by LPS-induced acute testis inflammation. In addition, MKP-1 staining was strongly detected in nuclei and peri-nuclear regions of cytoplasm in the Sertoli cells, and it was presented at Sertoli cell tight junctions (TJs) at stages VII-VIII after LPS treatment. Moreover, we demonstrated that MKP-1 was capable of attenuating LPS-induced decrease of occludin by interaction with p38 MAP kinase and IκBα molecules. Taken together, our data highlight that MKP-1 was an important endogenous suppressor of innate immune responses involved in the regulation of BTB barrier dynamic. This study thus might offer novel targets for treating inflammatory diseases in the testis.

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Section: Discussionsupporting

confidence: 92%

MKP-1 attenuates LPS-induced blood-testis barrier dysfunction and inflammatory response through p38 and IκBα pathways

et al. 2016

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“…This observation was in agreement with the findings obtained in PLS analysis ( Figure 6A). The role of the metabolites detected in this study has been described in numerous studies [23][24][25]. Earlier studies have reported The presented values are the mean of four replicates ± SD.…”

Section: Correlation Between Metabolite Profiles and No Inhibitory Acsupporting

confidence: 63%

Discrimination and Nitric Oxide Inhibitory Activity Correlation of Ajwa Dates from Different Grades and Origin

et al. 2016

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This study was aimed at examining the variations in the metabolite constituents of the different Ajwa grades and farm origins. It is also targeted at establishing the correlations between the metabolite contents and the grades and further to the nitric oxide (NO) inhibitory activity. Identification of the metabolites was generated using 1 H-NMR spectroscopy metabolomics analyses utilizing multivariate methods. The NO inhibitory activity was determined using a Griess assay. Multivariate data analysis, for both supervised and unsupervised approaches, showed clusters among different grades of Ajwa dates obtained from different farms. The compounds that contribute towards the observed separation between Ajwa samples were suggested to be phenolic compounds, ascorbic acid and phenylalanine. Ajwa dates were shown to have different metabolite compositions and exhibited a wide range of NO inhibitory activity. It is also revealed that Ajwa Grade 1 from the al-Aliah farm exhibited more than 90% NO inhibitory activity compared to the other grades and origins. Phenolic compounds were among the compounds that played a role towards the greater capacity of NO inhibitory activity shown by Ajwa Grade 1 from the al-Aliah farm.

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“…Indeed, we showed that iNOS, probably through NF-kB-dependent signaling cascades, is also involved in the regulation of MYD88 expression in the liver (6). Furthermore, the results of Kim et al (36), Lu et al (37), and Hammami et al (38) suggest that Gln may directly alter iNOS activity in vitro. In accordance with these findings, Esposito et al (39) also found that in ischemia/reperfusion models the beneficial effects of Gln supplementation on the liver were associated with protection against the induction of iNOS in the liver.…”

Section: Discussionmentioning

confidence: 55%

Oral Glutamine Supplementation Protects Female Mice from Nonalcoholic Steatohepatitis

Sellmann

Wei

Degen

et al. 2015

The Journal of Nutrition

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Anti‐inflammatory effects of glutamine on LPS‐stimulated human dental pulp cells correlate with activation of MKP‐1 and attenuation of the MAPK and NF‐κB pathways

Abstract: Glutamine exerted an anti-inflammatory effect via activation of MKP-1 and inhibition of the NF-κB and MAPK pathways in LPS-treated HDPCs.

Cited by 38 publications

References 32 publications

MKP-1 attenuates LPS-induced blood-testis barrier dysfunction and inflammatory response through p38 and IκBα pathways

MKP-1 attenuates LPS-induced blood-testis barrier dysfunction and inflammatory response through p38 and IκBα pathways

Discrimination and Nitric Oxide Inhibitory Activity Correlation of Ajwa Dates from Different Grades and Origin

Oral Glutamine Supplementation Protects Female Mice from Nonalcoholic Steatohepatitis

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