Anti-inflammatory effect of tribulusamide D isolated from Tribulus terrestris in lipopolysaccharide-stimulated RAW264.7 macrophages

Lee, Hyunhwa; Ahn, Eun‐Kyung; Hong, Seong-Su; Oh, Joa Sub

doi:10.3892/mmr.2017.7208

Cited by 38 publications

(27 citation statements)

References 30 publications

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“…Macrophages, as classical innate immune cells, play important roles in maintaining homeostasis such as phagocytizing foreign substances and secreting cytokines in the process of immunoreactivities ( 11 , 12 ). In addition to their classical function in immune system, the non-classical function of macrophage in peripheral, non-lymphoid organs has been widely investigated ( 13 ).…”

Section: Introductionmentioning

confidence: 99%

The Involvement of the Chemokine RANTES in Regulating Luminal Acidification in Rat Epididymis

Feng

Liu

et al. 2020

Front. Immunol.

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Background: A complex interplay between different cell types in the epithelium leads to activation of the luminal acidifying capacity of the epididymis, a process that is crucial for sperm maturation and storage. Basal cells sense the luminal angiotensin II (ANG II) and stimulate proton secretion in clear cells through nitric oxide (NO). Our previous study has shown the chemokine regulated upon activation normal T-cell expressed and secreted (RANTES) was expressed in the F4/80 positive macrophages of human epididymis. The objective of this study was to explore the involvement of RANTES in regulating the luminal acidification in the rat epididymis. Methods: The role of RANTES was investigated by in vivo perfusion with recombinant RANTES, Met-RANTES, and PBS of different pH values. Furthermore, rats vasectomy was performed to alter the epididymal luminal pH. RIA was used to measure the tissue homogenate ANG II concentration. Real time-PCR and western blot were employed to examine the expression levels of AGTR2, RANTES, CCR1, CCR5, and iNOS in epididymis. Results: RANTES was restricted to the basal macrophages of epididymal ducts and co-localized with its receptors CCR1 and CCR5. Both V-ATPase and iNOS were upregulated in the cauda epididymis after perfused with recombinant RANTES, while the antagonist Met-RANTES perfusion led to a complete abrogation of the increased expression of V-ATPase in the apical membrane of clear cells and iNOS in macrophages. Upon alkaline perfusion, RANTES expression was significantly increased and the apical accumulation of V-ATPase in the clear cells was induced in the cauda epididymis. The luminal pH in the cauda epididymis increased after vasectomy. The concentration of the ANG II and the expression levels of AGTR2, RANTES, CCR1, CCR5, and iNOS dropped in the cauda epididymis following vasectomy. Conclusion: Upon the activation of basal cells, RANTES might induce the NO release from macrophages by interacting with its receptors, which increases proton secretion by adjacent clear cells. Thus, RANTES is possible to participate in the crosstalk among basal cells, macrophages and clear cells for the fine control of an optimum acidic luminal environment that is critical for male fertility.

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Section: Introductionmentioning

confidence: 99%

The Involvement of the Chemokine RANTES in Regulating Luminal Acidification in Rat Epididymis

Feng

Liu

et al. 2020

Front. Immunol.

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“…The anti-inflammation effect of Tribulus terrestris L. contributes to its effectiveness in multiple diseases while the anti-inflammatory component of Tribulus terrestris L. is not well-known. Lee et al found that tribulusamide D, a compound isolated from Tribulus terrestris L., suppressed LPS-induced inflammatory response in RAW 264.7 cells via inhibition of P38 and NF- κ B signaling cascade [ 13 ]. N-trans- ρ -caffeoyl tyramine, which is also one of the components of Tribulus terrestris L., presented anti-inflammatory activity in LPS-stimulated RAW 264.7 cells by suppression of the protein levels of COX-2 and p-JNK [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

“…Tribulus terrestris L. (TT, Zygophyllaceae family), which contains active components including alkaloids, steroidal saponins, ﬂavonoids, tannins, amino acids, quinines, and phenolic compounds, is a commonly used traditional Chinese herbal medicine, also known as Bai Jili in Chinese, for treating various diseases including hypertension, edema, eye problems, sexual dysfunction, and rheumatoid arthritis in clinics for thousands of years [ 13 , 14 ]. The prosexual, cardiac-protective, muscle protective, neuroprotective, and osteoprotective effects of TT were most studied in the recent years [ 14 – 19 ], and the anti-inflammatory effect of TT might contribute to these broad range of biological effects [ 13 ]. In our previous studies, we have established multiple drug screening models using zebrafish, such as proangiogenesis [ 20 , 21 ], antiangiogenesis [ 22 ], cerebral hemorrhage [ 23 , 24 ], and neuroprotection [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Tribulus terrestris L. Extract Protects against Lipopolysaccharide-Induced Inflammation in RAW 264.7 Macrophage and Zebrafish via Inhibition of Akt/MAPKs and NF-κB/iNOS-NO Signaling Pathways

Zhao

Shi

Zhang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Inflammation response is a regulated cellular process and excessive inflammation has been recognized in numerous diseases, such as cardiovascular disease, neurodegenerative disease, inﬂammatory bowel disease, and cancer. Tribulus terrestris L. (TT), also known as Bai Jili in Chinese, has been applied in traditional Chinese medicine for thousands of years while its anti-inflammatory activity and underlying mechanism are not fully elucidated. Here, we hypothesize Tribulus terrestris L. extract (BJL) which presents anti-inflammatory effect, and the action mechanism was also investigated. We employed the transgenic zebrafish line Tg(MPO:GFP), which expresses green fluorescence protein (GFP) in neutrophils, and mice macrophage RAW 264.7 cells as the in vivo and in vitro model to evaluate the anti-inflammatory effect of BJL, respectively. The production of nitric oxide (NO) was measured by Griess reagent. The mRNA expression levels of inflammatory cytokines and inducible nitric oxide synthase (iNOS) were measured by real-time PCR, and the intracellular total or phosphorylated protein levels of NF-κB, Akt, and MAPKs including MEK, ERK, p38, and JNK were detected by western blot. We found that BJL significantly inhibited fin transection or lipopolysaccharide- (LPS-) induced neutrophil migration and aggregation in zebrafish in vivo. In mice macrophage RAW 264.7 cells, BJL ameliorated LPS-triggered excessive release of NO and transcription of inflammatory cytokine genes including tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β). BJL also reduced the LPS-induced elevations of intracellular iNOS and nuclear factor kappa B (NF-κB) which mediate the cellular NO and inflammatory cytokine productions, respectively. Moreover, LPS dramatically increased the phosphorylation of Akt and MAPKs including MEK, ERK, p38, and JNK in RAW 264.7 cells, while cotreatment BJL with LPS suppressed their phosphorylation. Taken together, our data suggested that BJL presented potent anti-inflammatory effect and the underlying mechanism was closely related to the inhibition of Akt/MAPKs and NF-κB/iNOS-NO signaling pathways.

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“…TT is used for kidney disorders by removing the gravel from the urine and stone in the bladder. [678910]…”

Section: Introductionmentioning

confidence: 99%

“…It has already been proven that TT plant extract has antioxidative, apoptosis inhibitory, and vasodilator properties, as well as protecting renal epithelial cells against damages due to oxalates and ethylene glycol consumption. [78910] The aim of this study was to investigate the protective effects of TT extracts against acute kidney injury (AKI) induced by mercuric chloride.…”

Section: Introductionmentioning

confidence: 99%

Effect of Tribulus terrestris in mercuric chloride-induced renal accumulation of mercury and nephrotoxicity in rat

Yadav

Sharma

Singh

et al. 2019

J Adv Pharm Technol Res

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Mercury generates free radicals and subsequently increases oxidative stress, which leads to renal injury. Tribulus terrestris (TT) has good anti-inflammatory and antioxidant properties. Hydroalcoholic extract of different dose of TT was evaluated against mercuric chloride-induced nephrotoxicity. Rats ( n = 6) were treated with TT at doses of 100, 200, and 300 mg/kg. Drugs were administered orally for 7 days. Single dose of mercuric chloride (5 mg/kg, intraperitoneal) on the 5 th day caused significant elevation of blood urea nitrogen, serum creatinine, malondialdehyde, liver fatty acid binding protein, kidney injury molecule-1, and kidney mercury level and fall in glutathione, superoxide dismutase, glutathione peroxidase, and histopathological changes in disease control as compared to normal control group ( P < 0.001). Dose of TT 200 and 300 mg/kg significantly ( P < 0.001) prevented the renal injury, and mercury accumulation in kidney tissues significantly decreases in higher dose, i.e., 300 mg/kg as compared to control group. Our result indicates that the treatment of TT exerted significant protection against renal damage induced by mercuric chloride possibly due to its antioxidant and anti-inflammatory properties and by decreasing the renal accumulation of mercury.

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Anti-inflammatory effect of tribulusamide D isolated from Tribulus terrestris in lipopolysaccharide-stimulated RAW264.7 macrophages

Cited by 38 publications

References 30 publications

The Involvement of the Chemokine RANTES in Regulating Luminal Acidification in Rat Epididymis

The Involvement of the Chemokine RANTES in Regulating Luminal Acidification in Rat Epididymis

Tribulus terrestris L. Extract Protects against Lipopolysaccharide-Induced Inflammation in RAW 264.7 Macrophage and Zebrafish via Inhibition of Akt/MAPKs and NF-κB/iNOS-NO Signaling Pathways

Effect of Tribulus terrestris in mercuric chloride-induced renal accumulation of mercury and nephrotoxicity in rat

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