Anti-inflammatory effect of stem bark of Paulownia tomentosa Steud. in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and LPS-induced murine model of acute lung injury

Lee, Jae Won; Seo, Kyeong Hwa; Ryu, Hyung Won; Yuk, Heung Joo; Park, Hyun Ah; Lim, Yourim; Ahn, Kyung Seop; Oh, Sei‐Ryang

doi:10.1016/j.jep.2017.08.028

Cited by 44 publications

(32 citation statements)

References 46 publications

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“…Protein bands were visualized using a LAS-4000 luminescent image analyzer (Fujifilm, Tokyo, Japan) and quantified by densitometry (Fuji Multi Gauge software version 3.0). The lung tissues were homogenized in a RIPA lysis buffer (1/10 w/v) containing a protease inhibitor cocktail (Sigma, St. Louis, MO, United States) ( Lee et al, 2018 ). For ELISA, the activities of phospho-ERK1/2 were assayed using commercially available ELISA kits (Abcam, Cambridge, United Kingdom) according to the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

Lignans Isolated From Flower Buds of Magnolia fargesii Attenuate Airway Inflammation Induced by Cigarette Smoke in vitro and in vivo

et al. 2018

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The flower buds of Magnolia fargesii, known traditionally as Xinyi, exert anti-inflammatory effects against inflammatory lung diseases such as COPD. Lignans isolated from Xinyi are an important group of plant-derived anti-inflammatory compounds. However, the mechanisms of action underlying their protective effects against COPD are not yet fully understood. Here, we showed that seven lignans (lignans 1–7) obtained from a CHCl3 fraction of Xinyi effectively suppress the inflammatory response in CSC-stimulated airway epithelial cells (in vitro) and in a mouse model of COPD established by exposure to CS and LPS. The CHCl3 fraction was found to inhibit CSC-induced IL-6 expression in human airway epithelial cells and to suppress the infiltration of inflammatory cells (neutrophils and macrophages) and secretion of inflammatory cytokines, such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the mouse model. Similarly, each of the seven lignans isolated from the CHCl3 fraction also suppressed the infiltration of inflammatory cells (neutrophils and macrophages) and secretion of inflammatory mediators such as reactive oxygen species (ROS), TNF-α, and IL-6 in vivo. Notably, all lignan compounds significantly suppressed both extracellular signal-related kinase (ERK) and Akt phosphorylation levels in CSC-stimulated human lung mucoepidermoid carcinoma (NCI-H292) cells. Of these, lignan 1 (dimethylpinoresinol) inhibited the expression of CSC-induced inflammatory cytokines (IL-1β, -6, and -8) in vitro in a dose-dependent manner by suppressing the activation of epidermal growth factor receptor (EGFR) and its downstream effectors, including ERK and Akt, in NCI-H292 cells. Our results show that the lignans isolated from Xinyi may prevent airway inflammatory diseases through the suppression of EGFR and its downstream effectors.

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Section: Methodsmentioning

confidence: 99%

Lignans Isolated From Flower Buds of Magnolia fargesii Attenuate Airway Inflammation Induced by Cigarette Smoke in vitro and in vivo

et al. 2018

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“…Paulownia tomentosa ( P. tomentosa ) is an extremely fast-growing tree species with multiple uses, and is widely cultivated in saline regions [1]. The bark, leaves, and flowers of Paulownia trees have been used in traditional medicine to treat infectious and inflammatory diseases in East Asia [2]. Pharmacological studies showed that the extracts from P. tomentosa could inhibit the protein tyrosine phosphatase 1B and α-glucosidase, which are important therapy targets of obesity and diabetes treatment [3], suppress the production of IL-6 and TNF-α in LPS-stimulated RAW 264.7 macrophages [2], and treat airway inflammation [4].…”

Section: Introductionmentioning

confidence: 99%

“…The bark, leaves, and flowers of Paulownia trees have been used in traditional medicine to treat infectious and inflammatory diseases in East Asia [2]. Pharmacological studies showed that the extracts from P. tomentosa could inhibit the protein tyrosine phosphatase 1B and α-glucosidase, which are important therapy targets of obesity and diabetes treatment [3], suppress the production of IL-6 and TNF-α in LPS-stimulated RAW 264.7 macrophages [2], and treat airway inflammation [4]. It has been also demonstrated that methanolic extracts of P. tomentosa flowers are active against EV71 of hand, foot, and mouth disease [5].…”

Section: Introductionmentioning

confidence: 99%

The enhanced immunological activity of Paulownia tomentosa flower polysaccharide on Newcastle disease vaccine in chicken

Yang

Zhang

Xu³

et al. 2019

Bioscience Reports

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The extracts of Paulownia tomentosa (P. tomentosa) exhibit multiple pharmacological activities. In the present study, P. tomentosa flower polysaccharides (PTFP) were extracted by water decoction and ethanol precipitation, and the immunologic modulations of PTFP against Newcastle disease (ND) vaccine was investigated in chickens. The results showed that in a certain range of concentrations, PTFP treatment can dose-dependently enhance lymphocyte proliferation. Then, 280 14-days-old chickens were randomly divided into seven groups, and vaccinated with ND vaccine except blank control (BC) group. At the first vaccination, chickens were orally administrated with PTFP at concentration ranging from 0 to 50 mg/kg once a day for 3 successive days, and the BC group was treated with physiological saline. The lymphocyte proliferation rate, serum antibody titer, and levels of interferon-γ (IFN-γ) were respectively measured on 7, 14, 21, and 28 days after the first vaccination. The results showed that PTFP at the suitable doses could significantly promote lymphocyte proliferation, enhance serum antibody titer, and improve serum IFN-γ concentrations. Taken together, these data indicated that PTFP could improve the immune efficacy against ND vaccine in chickens, and could be as the candidate of a new-type immune adjuvant.

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“…It has been reported that NF-κB is activated following the activation of its inhibitor IκB (24,25). Upregulation in the phosphorylation of IκB and NF-κB has been identified in the lung samples of OVA-induced allergic asthma mice (26).…”

Section: Resultsmentioning

confidence: 99%

Physalis peruviana L. inhibits ovalbumin‑induced airway inflammation by attenuating the activation of NF‑κB and inflammatory molecules

et al. 2019

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Physalis peruviana L. (PP) is well known for its various properties, including its antioxidant property. In our previous study, the protective effects of PP against cigarette smoke-induced airway inflammation were confirmed. The purpose of the present study was to evaluate the anti-inflammatory effect of PP against ovalbumin (OVA)-induced airway inflammation. Treatment with PP inhibited the numbers of eosinophils and the levels of inflammatory cytokines, including interleukin (IL)-4, IL-5 and IL-13, in the bronchoalveolar lavage fluid (BALF) of animal models with OVA-induced allergic asthma. PP also significantly decreased the production of total immunoglobulin E in the serum. Lung sections stained with hematoxylin and eosin revealed that the influx of inflammatory cells was decreased in the lungs of mice treated with PP compared with cells in the OVA group. The increased expression levels of monocyte chemoattractant protein-1 (MCP-1) and T cell marker KEN-5 were also reduced following PP treatment in the lung tissues compared with those in the OVA group. The PAS staining results showed that PP attenuated the overproduction of mucus in the lung. Additionally, western blot analysis revealed that PP significantly downregulated the activation of nuclear factor-κB/p38 mitogen-activated protein kinase/c-Jun N-terminal kinase, and upregulated the expression of heme oxgenase-1 in the lungs. In an in vitro experiment, PP effectively reduced the levels of LPS-stimulated MCP-1 in a concentration-dependent manner. Taken together, these results indicate that PP has considerable potential in the treatment of allergic asthma.

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Anti-inflammatory effect of stem bark of Paulownia tomentosa Steud. in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and LPS-induced murine model of acute lung injury

Cited by 44 publications

References 46 publications

Lignans Isolated From Flower Buds of Magnolia fargesii Attenuate Airway Inflammation Induced by Cigarette Smoke in vitro and in vivo

Lignans Isolated From Flower Buds of Magnolia fargesii Attenuate Airway Inflammation Induced by Cigarette Smoke in vitro and in vivo

The enhanced immunological activity of Paulownia tomentosa flower polysaccharide on Newcastle disease vaccine in chicken

Physalis peruviana L. inhibits ovalbumin‑induced airway inflammation by attenuating the activation of NF‑κB and inflammatory molecules

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