Anti-inflammatory effect of resveratrol on adjuvant arthritis rats with abnormal immunological function via the reduction of cyclooxygenase-2 and prostaglandin E2

Abstract

Rheumatoid arthritis (RA) is a chronic inflammatory disease with unknown etiology. The present study investigated the anti-inflammatory effect of resveratrol on rats with adjuvant arthritis (AA) with abnormal immunological function via the reduction of cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2). AA model rats were established by injection of complete Freund's adjuvant and alterations in the rats secondary paw swelling and the polyarthritic scores were observed. Pathological examination of joint tissues was observed by hematoxylin and eosin staining. The proliferation of spleen cells was examined using a 3-(4,5-dimethylthiazol-2‑yl)-2,5-diphenyltetrazolium bromide assay in vitro. The protein expression of COX-2 in the synovial tissues was detected by western blotting. The level of PGE2 in the serum was assayed using an ELISA kit. The results demonstrated that resveratrol (10 or 50 mg/kg) was able to significantly reduce paw swelling and decrease the arthritis scores. Compared with the AA model rats, a significant reduction in the proliferation of concanavalin A-stimulated spleen cells was observed, articular cartilage degeneration with synovial hyperplasia and inflammatory cell infiltration was suppressed and the production of COX-2 and PGE2 in AA rats was reduced by treatment with resveratrol. These results suggest that resveratrol has significant anti-inflammatory effects on AA rats, which may be associated with the reduction of COX-2 and PGE2 inflammatory mediators.

Synergistic Ameliorative Effects of Resveratrol with Leflunomide on Serum Levels of Inflammatory Biomarkers and Joint Damage in Rats with Adjuvant Arthritis

“…Also, they are in accordance with the recent studies that reported resveratrol showed significant cerebroprotective action against global cerebral ischemic/ reperfusion injury mediated by an antioxidant and anti-inflammatory mechanisms, and manifested as a significant reduction in oxidative stress markers as MDA, and inflammatory biomarkers as TNF-α, IL-6 and 10 and MPO enzymes [69,70]. Moreover, the laboratory and histological results of present study, are in agreement with other recent experimental study reported that the articular cartilage degeneration with synovial hyperplasia and inflammatory cell infiltration was significantly suppressed with also, a significant reduction in serum levels of COX-2 and PGE2 in adjuvant arthritic rats treated with resveratrol and suggested that resveratrol has a significant antiinflammatory effects; mediated by its ability to reduce the production of inflammatory biomediators COX-2 and PGE2 [71].…”

Resveratrol inhibits Src tyrosine kinase, STAT3, and Wnt signaling pathway in collagen induced arthritis model

“…Resveratrol has been shown to reduce the expression of MMP and RANKL from RA‐FLSs . Moreover, anti‐arthritic potentials have been shown in in vivo settings (various experimental arthritis models) . Riveiro‐Naveira et al have reported that resveratrol reduces the severity of arthritis and joint swelling in the CIA model.…”

Recent Advances on the Anti-Inflammatory and Antioxidant Properties of Red Grape Polyphenols: In Vitro and In Vivo Studies

“…In rabbit arthritis, intra-articular injection of RES dramatically reduced cartilage destruction [250]. On the other hand, in various models of experimental arthritis oral administration of RES reduced severity of disease, dampening release of proinflammatory cytokines, even including IL-17 [108,251,252].…”