Anti-Inflammatory Effect of Quercetagetin, an Active Component of Immature Citrus unshiu, in HaCaT Human Keratinocytes

Abstract

Citrus fruit contain various flavonoids that have multiple biological activities. However, the content of these flavonoids are changed during maturation and immature Citrus is known to contain larger amounts than mature. Chemokines are significant mediators for cell migration, while thymus and activation-regulated chemokine (TARC/CCL17) and macrophage-derived chemokine (MDC/CCL22) are well known as the typical inflammatory chemokines in atopic dermatitis (AD), a pruritic and chronic inflammatory skin disease. We reported recently that the EtOH extract of immature Citrus unshiu inhibits TARC and MDC production. Therefore, we investigated the activity of flavonoids contained in immature Citrus on TARC and MDC levels. As a result, among the various flavonoids, quercetagetin has stronger inhibitory effects on the protein and mRNA expression of TARC and MDC than other flavonoids. Quercetagetin particularly has better activity on TARC and MDC level than quercetin. In HPLC analysis, the standard peak of quercetagetin matches the peaks of extract of immature C. unshiu. This suggests that quercetagetin is an anti-inflammatory component in immature C. unshiu.

Magnetic molecularly imprinted polymer for the selective extraction of quercetagetin from Calendula officinalis extract

“…Quercetagetin (3, 5, 6, 7, 3', 4'-hexahydroxyflavone) [6], a characteristic flavonol compound with six additional phenolic hydroxyl groups based on the molecular structure of the flavones backbone, is widely distributed in the Calendula officinalis which was famous for its anti-inflammatory and anti-cancer [7].…”

In vitro antioxidant, anti-diabetic and antilipemic potentials of quercetagetin extracted from marigold (Tagetes erecta L.) inflorescence residues

“…1). There are many reports focused on its antioxidant activity (Gong et al 2012a, b;Xu et al 2014), antiinflammatory activity (Kang et al 2013) and copigment effect (Xu et al 2015). Some scientific researches reported that quercetin has strong inhibition of α-glucosidase, α-amylase and lipase (Dong et al 2012;You et al 2012).…”

Modified Da Chengqi granules improvement in immune function in early severe acute pancreatitis patients

“…Snakegourd root, a main component of snakegourd fruit, has immunoregulatory function that helps resist hypoxia, activates immunocytes, and increases the ability to defend against infections Xu et al, 2010;Yan et al, 2012). Immature orange fruit relieves constipation, displays anti-inflammatory and antioxidant effects (Menichini et al, 2011;Kang et al, 2013;Yu et al, 2013), promotes gut hormone secretion, and improves gastrointestinal motility (Wu et al, 2011;Lyu and Lee, 2013). Rhubarb and goldthread clear away heat-evil and expel superficial evils, and thus have a positive therapeutic impact on pancreatitis (Wu et al, 2000;Wang et al, 2007;Li et al, 2009).…”