Anti-inflammatory Effect of Ozone Therapy in an Experimental Model of Rheumatoid Arthritis

Tartari, Ana Paula Santos; Moreira, Felipe Figueiredo; Pereira, Mário César da Silva; Carraro, Emerson; Cidral-Filho, Francisco José; Salgado, Afonso Inoue; Kerppers, Ivo Ilvan

doi:10.1007/s10753-020-01184-2

Cited by 37 publications

(25 citation statements)

References 27 publications

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“…A handful of clinical reports confirm its efficacy in multiple sclerosis with down-regulation of the pro-inflammatory state and amelioration of oxidative stress [33-38] [292][293][294]. Similar results have been obtained in laboratory experiments with animal models of rheumatoid arthritis and psoriasis [282][283][284][285][286][287], and with fibromyalgia [295][296][297][298][299][300][301]. Although data is limited, given the presence of mitochondrial dysfunction, oxidative injury, and inflammation in affected neuronal populations in the neurodegenerative disorders [302][303][304][305], it would seem that these conditions too would be suitable candidates for ozone PC.…”

Section: The Case For Ozonesupporting

confidence: 61%

“…This effect can only be explained by the direct infusion of energy currents into mitochondria. Since abnormal inflammasome activation is a prominent feature of various chronic conditions like Alzheimer's, autoimmune disorders, cardiac and renal disease, as well as acute inflammatory syndromes like Covid-19, it has been suggested that ozone PC could modulate disease activity in these circumstances [168][169][170][171][172][173][174][175][176][177][178][179][180][181][182][183].…”

Section: Ozone Preconditioningmentioning

confidence: 99%

“…In that autoimmune disease is defined by chronic recurrent inflammatory episodes involving organs and tissues and characterized by similar dynamics as acute inflammation, i.e., activation of the immune system, elevated levels of pro-inflammatory cytokines, mitochondrial dysfunction and oxidative stress at the cellular level, it would be expected that they too would be ideal candidates for ozone PC [282][283][284][285][286][287][288][289][290][291]. A handful of clinical reports confirm its efficacy in multiple sclerosis with down-regulation of the pro-inflammatory state and amelioration of oxidative stress [33-38] [292][293][294].…”

Section: The Case For Ozonementioning

confidence: 99%

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Ozone Preconditioning: Waking up the dragon

Thorp¹,

Thorp²

2021

The GMS

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It is said that no discovery is complete until its implications have been fully realized. The identification of a previously unrecognized phenomenon says little of its potential effects. What appears as a stroke of good luck can culminate in disaster; misfortune may harbor unimagined potential. Discovery is a double-edged sword that manifests only over time. Nowhere is this aphorism more applicable than in contemporary medical science.

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Section: The Case For Ozonesupporting

confidence: 61%

Section: Ozone Preconditioningmentioning

confidence: 99%

Section: The Case For Ozonementioning

confidence: 99%

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Ozone Preconditioning: Waking up the dragon

Thorp¹,

Thorp²

2021

The GMS

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“…HCQ in particular reduces in the production of IL-1β, IL-6 and TNFα [ 127 ], thereby decreasing IFNγ release by CD56 BRIGHT NK cells, dampening overactive inflammatory responses. Several trials are also investigation the use of oxygen therapy in the early treatment of SARS-CoV-2 infection, as previous studies have shown that this treatment effectively decreases inflammation, reducing IL-12 and TNF-α, and increasing IL10 [ 128 , 129 ]. Numerous trials are investigating the possibility that the Bacille Calmette-Guérin (BCG) vaccine, used to protect young children against tuberculosis ( Mycobacterium tuberculosis ), may offer heterologous protection against SARS-CoV-2 through trained immunity [ 130 ].…”

Section: Immunological Features Of Covid-19mentioning

confidence: 99%

Natural Killer Cell Dysfunction and Its Role in COVID-19

Eeden

Khan

Osman

et al. 2020

IJMS

151

132

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When facing an acute viral infection, our immune systems need to function with finite precision to enable the elimination of the pathogen, whilst protecting our bodies from immune-related damage. In many instances however this “perfect balance” is not achieved, factors such as ageing, cancer, autoimmunity and cardiovascular disease all skew the immune response which is then further distorted by viral infection. In SARS-CoV-2, although the vast majority of COVID-19 cases are mild, as of 24 August 2020, over 800,000 people have died, many from the severe inflammatory cytokine release resulting in extreme clinical manifestations such as acute respiratory distress syndrome (ARDS) and hemophagocytic lymphohistiocytosis (HLH). Severe complications are more common in elderly patients and patients with cardiovascular diseases. Natural killer (NK) cells play a critical role in modulating the immune response and in both of these patient groups, NK cell effector functions are blunted. Preliminary studies in COVID-19 patients with severe disease suggests a reduction in NK cell number and function, resulting in decreased clearance of infected and activated cells, and unchecked elevation of tissue-damaging inflammation markers. SARS-CoV-2 infection skews the immune response towards an overwhelmingly inflammatory phenotype. Restoration of NK cell effector functions has the potential to correct the delicate immune balance required to effectively overcome SARS-CoV-2 infection.

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“…In rat model of experimental uveitis, ozone therapy decreases inflammation in histopathologic examination when compared with the sham group [ 16 ]. Ozone therapy has been proven effective in the treatment of experimental model of rheumatoid arthritis [ 17 ]. Additionally, ozone therapy contributes to the recovery of testicular damage in an experimental model of testicular torsion in rats by reducing oxidative stress [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of local ozone treatment on rats with anterior rectal resection and the possible mechanisms

Zhang

Chen

et al. 2021

BioMed Eng OnLine

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Background Anterior resection syndrome (ARS) is characterized by the diverse and interchangeable evacuatory symptoms that may occur following distal colorectal resection. We aimed to investigate the effect and potential mechanisms of ozone perfusion on rats with anterior rectal resection (ARR). Material and methods After establishment of rat ARR model, 20, 40 and 80 ug/ml ozone was used to treat rats by enema administration. The pathological examination of intestinal tissue was detected using hematoxylin–eosin staining. The rate of loose stools, minimum threshold volume of abdominal withdrawal reflex (AWR) and Bristol grade were used to evaluate the degree of abnormal defecation function. Subsequently, the levels of oxidative stress- and inflammation-related markers, 5-hydroxytryptamine (5-HT), inducible nitric oxide synthase (iNOS) and nitric oxide (NO) in the serum and intestinal tissue were determined with the corresponding kits. Meanwhile, the expression of nuclear factor kappa B (NF-κB) p65, transient receptor potential vanilloid (TRPV)1, TRPV4, iNOS and 5-HT receptor 3A (5-HTR3A) was determined with RT-qPCR and western blotting. Results Ozone administration (20 and 40 ug/ml) significantly alleviated the pathological changes of intestinal tissue-induced by ARR, accompanied by the decreased loose stools rate, Bristol score and increased abdominal withdraw reflex. However, 80 ug/ml of ozone intervention played opposite roles in the aforementioned changes with 20 and 40 ug/ml of ozone. Additionally, remarkably elevated reactive oxygen species (ROS), malonaldehyde (MDA), superoxide dismutase (SOD), 5-HT, iNOS and NO levels were observed in the ozone-treated groups (20 and 40 ug/ml), while high dose of ozone drastically improved ROS, MDA, 5-HT, iNOS and NO levels but reduced the activity of SOD. Consistently, the contents of inflammatory factors were decreased after low and middle doses of ozone administration. However, high dose of ozone aggravated the inflammatory injury. Moreover, 20 and 40 ug/ml ozone upregulated TRPV1 and TRPV4 expression but downregulated 5-HTR3A expression, which was restored after 80 ug/ml of ozone intervention. Remarkably, the levels of NF-κB p65 and iNOS were dose-dependently enhanced following ozone treatment. Conclusions Taken together, low concentration of ozone attenuated intestinal injury induced by ARR via balancing oxidative stress and inflammation, but high concentration of ozone exacerbated the intestinal injury, which might be related to the 5-HT and TRPV signaling.

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Anti-inflammatory Effect of Ozone Therapy in an Experimental Model of Rheumatoid Arthritis

Cited by 37 publications

References 27 publications

Ozone Preconditioning: Waking up the dragon

Ozone Preconditioning: Waking up the dragon

Natural Killer Cell Dysfunction and Its Role in COVID-19

Effect of local ozone treatment on rats with anterior rectal resection and the possible mechanisms

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