Anti-inflammatory Effect of Oxya chinensis sinuosa Ethanol Extract in LPS-induced RAW 264.7 Cells

Abstract

Although the grasshopper Oxya chinensis sinuosa has long been used as food in Korea, there is little data on its functional effects. In this study, we investigated the anti-inflammatory effect of O. c. sinuosa ethanol extract (OCE) in RAW 264.7 mouse macrophage cells treated with lipopolysaccharide (LPS) for induction of inflammation. First, we determined that there is no cytotoxicity at 2,000 μg/ml or less of OCE in RAW 264.7 cells. To evaluate the anti-inflammatory effects of OCE, we investigated expression levels of pro-inflammatory cytokines such as tumor necrosis factor (TNF)-α and interleukin (IL)-6, and pro-inflammatory enzymes such as inducible nitric oxide synthase (iNOS) and cyclo-oxygenase-2 (COX-2) in LPS-induced RAW 264.7 cells. In addition, we examined whether OCE could inhibit translocation of NF-κB p65 into the nucleus in LPS induced RAW 264.7 cells. As a result, we found that the mRNA and protein levels of TNF-α and IL-6 decreased in LPS-induced RAW 264.7 cells after treatment with OCE in a dose-dependent manner. In addition, we confirmed a 2,000 ug/ml concentration of OCE inhibited translocation of NF-κB p65 by immunnostaining and Western blot analysis, and a decrease in the protein expression levels of iNOS and COX-2. Accordingly, we suppose that OCE has an anti-inflammatory effect through down-regulation of TNF-α, IL-6, iNOS, and COX-2 related to NF-κB p65 inflammatory signaling pathways.

N-Acetyldopamine dimers from Oxya chinensis sinuosa attenuates lipopolysaccharides induced inflammation and inhibits cathepsin C activity

“…There is limited literature available on the isolation and biological activity evaluation of the compounds isolated from O. chinensis sinuosa [15] . However, a few studies were carried out in the O. chinensis sinuosa extract, elucidating their effect on the macrophage [30] and HepG2 cells viability [31] .…”

Antioxidant activity and antimicrobial activity of the grasshopper, Oxya chinensis sinuosa

“…예로부터 벼메뚜기는 기침, 천식, 백일해, 기관지염에 효 능이 있고 중풍과 경련을 치료하는데 도움을 주며 구황곤 충으로 이용되어 왔다. 벼메뚜기에 대한 국내 연구로는 메 뚜기류 추출물의 염증 조절작용 및 세포사멸보호 효과 (Park et al 2006), LPS로 염증이 유도된 RAW 264.7 세포에 벼 메뚜기 에탄올 추출물을 처리하여 벼메뚜기의 항염증 효 능에 대한 연구 (Yoon et al 2014), 벼메뚜기 건제품중의 지질열화에 함유색소성분이 미치는 영향 , 벼메뚜기 단백질의 영양가에 관한 연구 Mean ± SD (n = 3). Mean ± SD (n = 3).…”

Subacute Oral Toxicity Evaluation of Freeze-Dried Powder of Locusta migratoria

“…Locusta migratoria [Orthoptera: Acrididae (Linnaeus, 1758)], migratory locust, was reported to feed on grass and often causes serious damage to agricultural crops ( Tanaka et al, 2012 ). L. migratoria , Oxya chinensis sinuosa , and some species of the order Orthoptera have long been known to purportedly effective for the management of asthma, bronchitis, stroke, and Alzheimer’s disease ( Kim et al, 2015 ; Lee et al, 2018 ), and research has been conducted on the anti-inflammatory and cellular protective effects of O. chinensis sinuosa ( Park et al, 2006 ; Yoon et al, 2014 ). Studies have also been conducted on the nutritional components and antioxidant ( Kim et al, 1987 ) and antibacterial effects ( Kim et al, 2015 ) of L. migratoria .…”