N-Acetyldopamine dimers from Oxya chinensis sinuosa attenuates lipopolysaccharides induced inflammation and inhibits cathepsin C activity

Bahuguna, Ashutosh; Khaket, Tejinder Pal; Bajpai, Vivek K.; Shukla̽, Shruti; Park, InWha; Na, MinKyun; Huh, Yun Suk; Han, Young‐Kyu; Kang, Sun Chul; Kim, Myung‐Hee

doi:10.1016/j.csbj.2022.02.011

Cited by 7 publications

(3 citation statements)

References 44 publications

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“…Oxya chinensis sinuosa (OC) is a well-known edible insect ( Bahuguna et al, 2022 ; Kim et al, 2020 ). OC is an incomplete metamorphic insect of the genus Orthoptera of the order Orthoptera, with a body length of 21–35 mm.…”

Section: Introductionmentioning

confidence: 99%

Oxya chinensis sinuosa (OC) Extracts Protects ARPE-19 Cells against Oxidative Stress via Activation of the Mitogen-Activated Protein Kinases (MAPKs)/ Nuclear Factor-κB (NF-κB) Pathway

Kim,

Choi,

Kweon

et al. 2024

Food Sci Anim Resour

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Oxya chinensis sinuosa (OC) is a well-known edible insect. Several researches on the health benefits of OC consumption have been performed to date; however, their effect on eye health remains largely unknown. This study aimed to assess the protective effects of OC extracts on the oxidative stress on the retinal pigment epithelium (RPE) cells. Oxidative damage has been identified as one of the key regulatory factors in age-related macular degeneration. H 2 O 2 -induced reactive oxygen species (ROS) production, a well-known oxidative stress factor, can cause cell death in retinal pigment epithelia cells. In this study, we found that three OC extracts effectively prevented H 2 O 2 -induced ROS production and subsequent death of ARPE-19 cells in a dose-dependent manner. In addition, the OC extracts inhibited the phosphorylation of mitogen-activated protein kinases including p38, JNK, and ERK. The OC extracts restored IκBα degradation induced by H 2 O 2 , indicating that OC extracts suppressed the activation of nuclear factor-κB. Furthermore, the three OC extracts were shown to have antioxidant effects by up-regulating the intracellular expression of key antioxidant proteins such as SOD, NQO, and HO-1. Here we demonstrated the antioxidant and anti-apoptotic effects of the OC extracts on ARPE-19, indicating their potential role in improving eye health. These results suggest that three OC extracts plays a critical role in oxidative stress-induced cell death protects in ARPE-19 cells.

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Section: Introductionmentioning

confidence: 99%

Oxya chinensis sinuosa (OC) Extracts Protects ARPE-19 Cells against Oxidative Stress via Activation of the Mitogen-Activated Protein Kinases (MAPKs)/ Nuclear Factor-κB (NF-κB) Pathway

Kim,

Choi,

Kweon

et al. 2024

Food Sci Anim Resour

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“…The molecular docking was performed by Autodock 4.2, and binding energy was predicted to evaluate the affinity of the target proteins and active components. The best binding conformations were selected from 50 lowest energy docked structures based on a criterion combining the lowest binding energy and root-mean-square deviation values (RMSD <1) ( Bahuguna et al, 2022 ), and were displayed by PyMOL. Here, if the binding energy of the ligand <0, the receptors bind spontaneously, and if the binding energy is ≤ −5.0 kcal/mol, they form a stable docking ( Guo et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

A novel colchicine-myricetin heterozygous molecule: design, synthesis, and effective evaluations on the pathological models of acute lung injury in vitro and in vivo

Yan

Wei

et al. 2023

Front. Pharmacol.

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Acute lung injury (ALI) is an inflammatory condition and there are no effective treatments. A novel new compound----colchicine-myricetin hybrid (CMyrH) was herein designed and synthesized. To evaluate the activity of CMyrH in ALI, we used a bleomycin (BLM) induced BEAS-2B injury model in vitro and established a well-recognized rat model of BLM-induced lung injury in vivo. The results demonstrated that colchicine-myricetin hybrid protected BEAS-2B cells against BLM-induced cell injury in an increased dose manner, and reduced wet/dry weight ratio, histological scoring, and inflammation cytokines IL-1β, IL-6, IL-18, and TNF-α levels of lung tissue of the rats. Furthermore, we found colchicine-myricetin hybrid inhibited caspase-1, ASC, GSDMD, and NLRP-3 expression in vivo. Meanwhile, we used molecular docking to analyze the binding mode of colchicine-myricetin hybrid and human neutrophil elastase (HNE), it revealed that colchicine-myricetin hybrid showed strong binding affinity toward human neutrophil elastase when compared to its parent molecules. In conclusion, It is suggested that colchicine-myricetin hybrid antagonized acute lung injury by focusing on multi-targets via multi-mechanisms, and might be served as a potential therapeutic agent for acute lung injury.

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“…The representative compound 22 exhibited an IC 50 value of 0.026 μM with a selectivity index value of at least >1538. Interestingly, 1,4-benzodioxan derivatives have been also found to demonstrate anti-inflammatory activities that could effectively reduce the expression of pro-inflammatory mediator in LPS-stimulated macrophages. − …”

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confidence: 99%

Benzodioxane Carboxamide Derivatives As Novel Monoamine Oxidase B Inhibitors with Antineuroinflammatory Activity

Sun,

Wang,

Jiang

et al. 2024

ACS Med. Chem. Lett.

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In this study, a series of N-phenyl-2,3dihydrobenzo[b][1,4]dioxine-6-carboxamide derivatives were designed, synthesized, and evaluated for their inhibitory activities against human MAO-B (hMAO-B). The structure−activity relationship (SAR) was investigated and summarized. Compoundshowed the most potent inhibitory activity with an IC 50 value of 0.0083 μM and the selectivity index (IC 50 (hMAO-A)/IC 50 (hMAO-B)) was >4819. Kinetics and reversibility studies confirmed that compound 1l acted as a competitive and reversible inhibitor of hMAO-B. Molecular docking studies revealed the enzyme−inhibitor interactions, and the rationale was provided. Additionally, compound 1l could effectively inhibit the release of NO, TNF-α, and IL-1β in both LPS-and Aβ 1−42 -stimulated BV2 cells and attenuate the cytotoxicity induced by Aβ 1−42 . Since compound 1l exhibited low neurotoxicity, we believe that the hit compound with dual activities of inhibiting MAO-B and antineuroinflammation could be further investigated as a novel potential lead for future studies in vivo.

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N-Acetyldopamine dimers from Oxya chinensis sinuosa attenuates lipopolysaccharides induced inflammation and inhibits cathepsin C activity

Cited by 7 publications

References 44 publications

Oxya chinensis sinuosa (OC) Extracts Protects ARPE-19 Cells against Oxidative Stress via Activation of the Mitogen-Activated Protein Kinases (MAPKs)/ Nuclear Factor-κB (NF-κB) Pathway

Oxya chinensis sinuosa (OC) Extracts Protects ARPE-19 Cells against Oxidative Stress via Activation of the Mitogen-Activated Protein Kinases (MAPKs)/ Nuclear Factor-κB (NF-κB) Pathway

A novel colchicine-myricetin heterozygous molecule: design, synthesis, and effective evaluations on the pathological models of acute lung injury in vitro and in vivo

Benzodioxane Carboxamide Derivatives As Novel Monoamine Oxidase B Inhibitors with Antineuroinflammatory Activity

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