Anti-inflammatory effect of lysozyme from hen egg white on mouse peritoneal macrophages

Tagashira, Ayuka; Nishi, Kosuke; Matsumoto, Shimpei; Sugahara, Takuya

doi:10.1007/s10616-017-0184-2

Cited by 35 publications

(39 citation statements)

References 33 publications

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“…In previous study, we revealed that lysozyme from hen egg white suppresses production of inflammatory cytokines through inhibiting phosphorylation of JNK, one of MAP kinase family, in LPS-induced hyperinflammatory macrophages in vitro (Tagashira et al 2018). Therefore, we investigated the anti-inflammatory effect of lysozyme in vivo.…”

Section: Discussionmentioning

confidence: 98%

“…3, lysozyme significantly inhibited amounts of IL-6 and TNF-a levels in serum. It has been revealed that lysozyme suppresses production of IL-6 and TNF-a in hyperinflammatory macrophages (Tagashira et al 2018). These outcomes suggest that lysozyme could act on macrophages and suppress them in vivo.…”

Section: Discussionmentioning

confidence: 98%

“…Lysozyme also has an anti-inflammatory effect by binding LPS to form a complex and inhibits inflammatory reactions (Takada et al 1994). Moreover, our previous study also revealed that lysozyme inhibits phosphorylation of c-Jun N-terminal kinase (JNK) to suppress the expression of pro-inflammatory cytokine genes in hyperinflammatory macrophages (Tagashira et al 2018). These facts suggest that lysozyme has immune-regulatory effects through various mechanisms in vitro.…”

Section: Introductionmentioning

confidence: 93%

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Lysozyme from hen egg white ameliorates lipopolysaccharide-induced systemic inflammation in mice

2019

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Lysozyme is an anti-bacterial protein that is widely distributed in nature. Our previous studies revealed that lysozyme shows anti-inflammatory effect on hyperinflammatory macrophages in vitro. The effect of lysozyme on lipopolysaccharide-induced inflammation model mice was examined in this study. Oral administration of lysozyme at 2250 mg/kg body weight/day (high-dose group) significantly suppressed interleukin (IL)-6 and tumor necrosis factor-a levels in the serum. IL-6 level in the spleen was significantly suppressed by lysozyme at 450 mg/kg body weight/day (middle-dose group) and high-dose group due to the suppression of gene expression level. The gene expression levels of IL-1b and IL-12 were also decreased by lysozyme in the high-dose group. In addition, lysozyme significantly suppressed IL-6 level in the liver in the high-dose group. Our findings suggest that lysozyme mitigates inflammatory condition in vivo by suppressing inflammatory cytokine levels in serum and organs from LPS-induced inflammation model mice.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 93%

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Lysozyme from hen egg white ameliorates lipopolysaccharide-induced systemic inflammation in mice

2019

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“…Bacterial sensitivity to lysozyme is due to the activation of innate components of the immune system, such as increased neutrophil activation during inflammation [3, 22]. It has been reported that lysozyme may possess an anti-inflammatory effect via inhibiting JNK phosphorylation [23]. Furthermore, lysozyme is capable of enhancing intestinal SIgA secretion, cause macrophage activation, and promote rapid clearance of bacterial pathogens [2, 13, 22].…”

Section: Introductionmentioning

confidence: 99%

Dietary lysozyme supplement alters serum biochemical makers and milk metabolite profile of sows via gut microbiota

Zhou

Xiong

Zou

et al. 2018

Preprint

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24Lysozyme is an important antimicrobial agent with promising future in replacing antibiotics in 25 livestok production.The aim of current study was to determine variations in sow's gut microbiota, 26 serum immunity and breast milk metabolite profile mediated by lysozyme 27 supplementation.Thirty-six pregnant sows were assigned to a control group without 28 supplementation and two treatments with 0.5 g/kg and 1.0 g/kg lysozyme provided in formula feed 29 for 21days. Microbiota analysis based on 16s RNA high-throughput sequencing and untargeted 30 liquid chromatography tandem mass spectrometry were applied and combined in analysis. Serum 31 biochemical indicators and immunoglobulins were also determined.Sows received 1.0kg/t 32 lyszoyme treatment shown significant redution in microbial diversity. Spirochaetes, Euryarchaeota 33and Actinobacteria significantly increased while Firmicutes showed a remarkable reduction in 34 1.0kg/t treated group compared with control. Pyrimidine metabolism,Purine metabolism and 35Amino acid related enzymes were significantly upregulated in 1.0kg/t lysozyme treated group. 36The richness of gram-positive bacteria were significantly down-regulated by lysozyme 37 treatments.Serum aspartate transaminase (AST) activity was significantly un-regulated.Serum IgM 38 levels were significantly higher in the 1.0 kg/t group compared with control, while IgA levels was 39 significantly lower in 1.0kg/t group.Over thirty metabolites from sow's breast milk including 40 L-Glutamine,creatine and L-Arginine were sigficantly altered by lysozyme treatment.There 41 existed crucial correlations among gut microbiota, serum immunity and breast milk metabolites 42where lactobacillus and prevotella may play a key role in lysozyme mediated host-microbial 43 interactions. Overall, lysozyme supplementation could effectively improve the composition, 44 metabolic functions and phenotypes of sow's gut microbiota and it also benefit sows with better 45 immune status and breast milk composition. 46 47 Importance: Enteric infections caused by pathogens have a significant negative effect on neonatal 48 survival and animal health in swine production. The application of antibiotics in feeds at 49 subtherapeutic levels could improve performance and overall health and is used extensively 50 throughout the industry. However, abuse of antibiotics is contributing to the high level of drug 51 resistance in microbial communities and rising concerns regarding human health. Here, we 52 revealed that lysozyme supplementation could effectively improve the composition, metabolic 53 functions and phenotypes of sow's gut microbiota and it also benefit sows with better immune 54 status and breast milk composition. These findings confirmed that lysozyme could be a suitable 55 alternative to antibiotics in swine production. 56 57 58 59 60 lysozyme alters sow's gut microbiota and health 61 62 63 64 65 66 67

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“…Lysozyme is effective in the treatment of diabetic nephropathy through the inhibition of IL-6 production and reduction of macrophage recruitment during inflammation [19]. In human monocytes, it transcriptionally inhibits the TNF-α and IL-1β pathways [20], and in mouse macrophages stimulated with lipopolysaccharide, it suppresses the production of TNF-α and IL-6 [21]. Lysozyme directly inhibits inflammatory mediators, which are key factors for development of non-infectious sore throat.…”

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confidence: 99%

Lysozyme in the treatment of non-infectious sore throat

Glamočlija

Mehić

Sukalo

et al. 2020

Bosn J of Basic Med Sci

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Sore throat is a common reason for seeking medical help [1]. It can have infectious and non-infectious aetiology [2]. The term “sore throat” is usually associated with an infectious agent (bacteria, viruses, and less commonly fungi) [1,3,4]. The terms “throat discomfort“,“throat irritation“ or “functional dysphonia due to the occupational diseases“ (which represents voice disorder) are often used in the same sense as a non-infectious sore throat [2,3]. It is identified by the exclusion of infectious aetiology symptoms accompanied by persistent sore throat symptoms (tingling and scratching sensation in the throat, hoarseness or muffled voice, foreign body sensation in the throat, coughing, difficulty in swallowing accompanied by pain) [2,5]. Read the full text [PDF]

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Anti-inflammatory effect of lysozyme from hen egg white on mouse peritoneal macrophages

Cited by 35 publications

References 33 publications

Lysozyme from hen egg white ameliorates lipopolysaccharide-induced systemic inflammation in mice

Lysozyme from hen egg white ameliorates lipopolysaccharide-induced systemic inflammation in mice

Dietary lysozyme supplement alters serum biochemical makers and milk metabolite profile of sows via gut microbiota

Lysozyme in the treatment of non-infectious sore throat

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