Anti-Inflammatory Effect of 3-Methylcarbazoles on RAW 264.7 Cells Stimulated with LPS, Polyinosinic-Polycytidylic Acid and Pam3CSK

Taechowisan, Thongchai; Chanaphat, Srisakul; Ruensamran, Wanwikar; Phutdhawong, Weerachai

doi:10.4236/aim.2012.22013

Cited by 18 publications

(11 citation statements)

References 27 publications

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“…1-methoxy-3-methylcarbazole (3) Streptomyces sp. LJK109 Alpinia galanga Swartz [13] 7. 1-O-methyl emodin (64) Aspergillus fumigatus, Gaeumannomyces sp.…”

Section: -Epichaetomugilin I (57)mentioning

confidence: 99%

“…Streptomyces sp. LJK109 Alpinia galanga Swartz [13] 11. 3β,5α-dihydroxy-6β-methoxyergosta-7,22-diene (39) Trichoderma sp.…”

Section: -Methylcarbazole (2)mentioning

confidence: 99%

“…Among the 11 reported compounds, diaporindene C (10) (IC 50 4.2 µM) and D (11) (IC 50 4.2 µM) were the most potent inhibitors of LPS-induced NO production in raw 264.7 cell lines. Pseurotin A (1) was also found to be highly inhibitory (IC 50 5.20 µM) exhibiting indirect AI activity by suppressing the LPS-induced pro-inflammatory factors in BV2 microglial cells [13,25,29,35,45].…”

Section: Ai Alkaloids and Benzophenonesmentioning

confidence: 99%

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Prospective Leads from Endophytic Fungi for Anti-Inflammatory Drug Discovery

2020

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A wide array of therapeutic effects has been exhibited by compounds isolated from natural sources. “Bio-actives of endophytic origin” is a recently explored area that came into recognition over the last 2 decades. Literature search on the secondary metabolites of endophytes have shown several pharmacologically active compounds especially anti-inflammatory compounds, which have been reviewed in the present paper. The article is structured based on the chemical classification of secondary metabolites. The compounds were identified to possess activity against a total of 16 anti-inflammatory targets. The most common targets involved were NO, TNF-α, and inhibition of total ROS. Further, the article gives a detailed insight into the compounds, their endophytic source, and anti-inflammatory target as well as potency. The contents of the article cover all the scientific reports published until Feb. 2019. Thus 118 compounds and 6 extracts have been reported to be obtained from endophytic sources showing anti-inflammatory activities. Amongst these, herbarin, periconianone A, and periconianone B were identified as the most potent compounds in terms of their IC50 values against NO inhibition.

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“…1-methoxy-3-methylcarbazole (3) Streptomyces sp. LJK109 Alpinia galanga Swartz [13] 7. 1-O-methyl emodin (64) Aspergillus fumigatus, Gaeumannomyces sp.…”

Section: -Epichaetomugilin I (57)mentioning

confidence: 99%

“…Streptomyces sp. LJK109 Alpinia galanga Swartz [13] 11. 3β,5α-dihydroxy-6β-methoxyergosta-7,22-diene (39) Trichoderma sp.…”

Section: -Methylcarbazole (2)mentioning

confidence: 99%

Section: Ai Alkaloids and Benzophenonesmentioning

confidence: 99%

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Prospective Leads from Endophytic Fungi for Anti-Inflammatory Drug Discovery

2020

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“…These microorganisms were conserved by sub-culturing in nutrient agar medium. Antimicrobial assay of these compounds were studied through agar diffusion technique by determining zone of inhibition [17][18][19]. Prior to activity, petri dishes were sterilized at 160 °C for 1.5 h in hot air oven.…”

Section: Determination Of Zone Of Inhibitionmentioning

confidence: 99%

Synthesis and biological evaluation of triphenyl-imidazoles as a new class of antimicrobial agents

et al. 2018

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Newer triphenyl-imidazole derivatives (4a-h) were synthesized in good yields by the reaction of benzil and substituted benzaldehydes in equimolar quantities and refluxing the product with acetyl chloride thereafter. Structures were confirmed by using FT-IR, 1H NMR and 13C NMR spectroscopic methods. All the synthesized compounds were tested for their antimicrobial activity using agar diffusion technique against Gram positive (Staphhylococcus aureus and Bacillus subtilis), Gram negative (Escherichia coli and Pseudomonas aureginosa) as well as Fungal strain (Candida albicans). Interestingly compounds 4a, 4b, 4f and 4h showed significant antibacterial activity, whereas compound 4b was found to have remarkable activity against the fungal strain. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of most active compounds were determined by broth dilution method and compound 4b emerged to have potent activities against most of the strains having MIC in the range of 25-200 µg/mL. To check the possible toxicities of the most active compounds, they were orally administered in rats and the concentration of liver enzymes serum glutamic-oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT) and alkaline phosphatase (ALKP) were determined. Compound 4h showed significant increase in the enzymes level depicting the hepatotoxicity. The structure-activity relationship studies showed the importance of electron withdrawing groups at the distant phenyl ring at ortho and para positions as the compounds having chloro or nitro at these positions tend to be more active than the compounds with electron releasing groups such as methoxy. These compounds may act as lead compounds for further studies and appropriate modification in their structure may lead to agents having high efficacy with lesser toxicity.

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“…Endophytes secrete antibiotics or hydrolytic enzymes to prevent colonization of microbial plant pathogens (Alvin et al 2014). The novel antimicrobial metabolites from endophytes are now becoming an alternative to overcome the increasing levels of drug resistance (Ferlay et al 2010;Taechowisan et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Biotechnological applications of the medicinal plant Pseudobrickellia brasiliensis and its isolated endophytic bacteria

Cardoso

Campos

Santos

et al. 2020

J. Appl. Microbiol.

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Aim This study aimed to isolate Pseudobrickellia brasiliensis endophytic bacteria and evaluate the production of hydrolytic enzymes and antibiotics by these bacterial strains. The study also measured the antibacterial activity of P. brasiliensis. Methods and Results Thirteen endophytic bacteria strains were isolated from stem and leaf fragments of P. brasiliensis. Extracellular enzyme production by the isolated endophytic bacteria was evaluated in an agar plate‐based assay. The highest protease production was achieved by Bacillus subtilis P4 in alkaline medium. Antimicrobial activity of endophytic bacteria and P. brasiliensis extracts was investigated using microbroth dilution. An MIC value of 1000 μg ml−1 against Pseudomonas aeruginosa was found for B. subtilis P3, B. subtilis P5, Pseudomonas sp. P8 and Pseudomonas sp. P12. Leaf extract of P. brasiliensis showed the highest antibacterial activity against P. aeruginosa, with an MIC value of 0·781 mg ml−1. Conclusions Pseudobrickellia brasiliensis is a source of bacterial endophytes, which can produce antibacterial compounds and enzymes. This work also demonstrated the antibacterial potential of P. brasiliensis. Significance and Impact of the Study This is the first study that revealed the antibacterial activity of P. brasiliensis and bioactive metabolite production by P. brasiliensis endophytic bacteria.

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Anti-Inflammatory Effect of 3-Methylcarbazoles on RAW 264.7 Cells Stimulated with LPS, Polyinosinic-Polycytidylic Acid and Pam3CSK

Cited by 18 publications

References 27 publications

Prospective Leads from Endophytic Fungi for Anti-Inflammatory Drug Discovery

Prospective Leads from Endophytic Fungi for Anti-Inflammatory Drug Discovery

Synthesis and biological evaluation of triphenyl-imidazoles as a new class of antimicrobial agents

Biotechnological applications of the medicinal plant Pseudobrickellia brasiliensis and its isolated endophytic bacteria

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