Anti‐inflammatory drugs

Arman, C. G. Van

doi:10.1002/cpt1974165part2900

Cited by 59 publications

(24 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The influx and activation of neutrophils [PMN (polymorphonuclear leukocytes)] are essential for induction of inflammatory processes (1,2). The chemoattractant N-formylmethionylleucylphenylalanine (fMet-Leu-Phe) and products of complement activation such as Ca recruit and activate the PMN to release active oxygen species and undergo degranulation (2,3).…”

mentioning

confidence: 99%

Inhibition by prostaglandins of leukotriene B4 release from activated neutrophils.

Ham¹,

Soderman²,

Zanetti³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

246

114

View full text Add to dashboard Cite

Chemoattractant N-formylmethionylleucylphenylalanine (fMet-Leu-Phe) in the presence of cytochalasin B stimulates the release of leukotriene B4 (LTB4), superoxide (02) The influx and activation of neutrophils [PMN (polymorphonuclear leukocytes)] are essential for induction of inflammatory processes (1, 2). The chemoattractant N-formylmethionylleucylphenylalanine (fMet-Leu-Phe) and products of complement activation such as Ca recruit and activate the PMN to release active oxygen species and undergo degranulation (2,3). Such actions in concert with increased levels of prostaglandin E2 (PGE2) and prostacyclin (PGI2) lead to the cardinal symptoms of acute inflammation (3)(4)(5). Leukotriene B4 (LTB4), a product of the 5-lipoxygenase pathway, is itself a potent chemoattractant for PMN (6) and promotes their accumulation and adherence to the vascular endothelium proximal to the area of inflammation (3,7). Whether these actions of LTB4 supplement or mediate the effects of other chemoattractants remains unknown.Pharmacological levels of PGs inhibit superoxide (02) production induced by fMet-Leu-Phe in the presence of cytochalasin B (2) by an action purported to depend on increased cyclic AMP production (8). A similar cyclic AMP-dependent inhibition of lysosomal enzyme release has been observed by using large amounts of PGs (2, 8). Pharmacological levels of PGI2 (2.8 X 10-6 M) have also been reported to cause a partial inhibition (45%) of LTB4 release from human PMN activated with serum-treated zymosan (9).The studies in this report indicate that fMet- was obtained from J. Rokach (Merck Frosst) (10); MMM-I-135 (10,10-difluoro-13-dehydroprostacyclin) was a gift from J. Fried (University of Chicago) (11).The incubation buffer contained 130 mM NaCl, 5.5 mM KCl, 0.6 mM Na2HPO4, 0.6 mM NaH2PO4, 10 mM glucose, 1.0 mM CaC12, and 25 mM Tris HCl adjusted to pH 7.4 (12). Preparation of Cells. Rat PMN were obtained by peritoneal lavage with 40 ml of 0.9% NaCl/10 mM EDTA, pH 7.0, 16-24 hr after aseptic induction (13). PMN were recovered by centrifugation and the pellet was washed once in calcium-free incubation buffer, subjected to lysis buffer (14) for 5 min, rewashed in incubation buffer, and resuspended at a concentration of 5 x 106 cells per ml.Human peripheral PMN were isolated from fresh citrated human blood as described (15) and lysed as were the rat cells.Both preparations were >95% PMN.Incubation Conditions. Two-milliliter aliquots of PMN suspension were preincubated with calcium for 3 min at 37°C and then PGs or solvent was added for 5 min. Cytochalasin B (5 ,ug/ ml) was next added for 5 min, followed by addition of 10 ,uM fMet-Leu-Phe for 5 min. Dimethyl sulfoxide was at a final concentration of 0.7%. The incubations were terminated by centrifugation at 260 X g for 5 min at 0°C. The supernatants were analyzed for (5S)-5-hydroxy-6,8,11,14-icosatetraenoic acid (5-HETE), LTB4, and enzymes. Cell pellets were assayed for cyclic AMP.

show abstract

mentioning

confidence: 99%

Inhibition by prostaglandins of leukotriene B4 release from activated neutrophils.

Ham¹,

Soderman²,

Zanetti³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

246

114

View full text Add to dashboard Cite

show abstract

“…A atividade anti-inflamatória foi avaliada pelo modelo de edema de orelha induzido por óleo de cróton, 9 em camundongos Swiss machos (25-30 g). O edema de orelha foi induzido pela aplicação tópica de 20 μL de óleo de cróton comercial Sigma Aldrich (OC, 200 μg) diluído em acetona/água (7:3) na superfície interna da orelha esquerda do camundongo.…”

Section: Avaliação Da Atividade Anti-inflamatóriaunclassified

CHEMICAL CONSTITUENTS AND EVALUATION OF ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF ROOTS OFSabicea brasiliensisWernh (Rubiaceae).

et al. 2014

View full text Add to dashboard Cite

Sabicea brasiliensis Wernh (Rubiaceae). The phytochemical investigation of Sabicea brasiliensis roots led to the isolation of 5-O-caffeoylquinic acid, 3,5-and 4,5-O-dicaffeoylquinics acids, scopoletin, ursolic acid, a mixture of β-sitosterol, stigmasterol and campesterol, daucosterol and saccharose. The structures of the isolated compounds were assigned on the basis of one-and twodimensional NMR spectroscopic methods and by comparison with literature data. The anti-inflammatory and antioxidant activities of the crude methanolic extract and its fractions were analyzed.Keywords: Sabicea brasiliensis; caffeoylquinic acid; anti-inflammatory activity. INTRODUÇÃOO gênero Sabicea, pertencente à família Rubiaceae, à subfamília Ixoroideae e à tribo Sabiceae, é composto por aproximadamente 100 espécies distribuídas pela América Tropical e África, sendo que cerca de 45 a 50 espécies estão presentes na região neotropical.1 Espécies deste gênero são usadas na medicina popular para o tratamento da dor de estômago e de dente, disenteria, malária, reumatismo, prisão de ventre, epilepsia, febre, vômitos, insônia e ainda para combater doenças venéreas e como depurativo do sangue, além do uso como adoçante.2 Entretanto, apesar do amplo uso popular de plantas deste gênero, apenas Sabicea grisea foi estudada fitoquimicamente.Das folhas desta espécie foi isolado o octacosanol, 3 composto detentor de atividades biológicas importantes como antioxidante, ergogênica e antiparkinsoniana, 4 sendo que o extrato bruto e a fração hexânica de onde ele foi retirado apresentaram atividades antinociceptiva e anti-inflamatória, com inibição da dor induzida por ácido acético em camundongos, assim como o próprio octacosanol. 3A espécie Sabicea brasiliensis é um arbusto com aproximadamente 80 cm de altura conhecido popularmente como sangue-de-cristo, de ocorrência nas regiões de cerrado do Brasil 1 que, além de ser usado pela população essencialmente como alimento, 5 é empregado no tratamento de infecções nos órgãos genitais de mulheres.6 Esta espécie possui leveduras que são aplicadas no controle de doenças pós-colheita em laranja e pêssego. 7A escassez de estudos químicos com espécies do gênero Sabicea, a ausência dos mesmos acerca da espécie S. brasiliensis e os interessantes usos populares que tais plantas possuem motivaram a realização do presente trabalho, no qual é relatado o isolamento e identificação de três ácidos cafeoilquínicos, uma cumarina, um triterpeno, três esteroides, um esteroide glicosilado e um dissacarídeo. Com base no uso popular desta espécie em infecções genitais, a avaliação da atividade anti-inflamatória foi efetuada e, também, devido ao isolamento de compostos fenólicos da classe dos ácidos cafeoilquínicos, foi realizado teste para deferir o potencial antioxidante do extrato bruto e frações de S. brasiliensis. PARTE EXPERIMENTAL Procedimentos experimentais geraisOs espectros de RMN (uni-e bidimensionais) foram obtidos em espectrômetro Varian, modelo Mercury plus BB, operando a 300 MHz para 1 H e 75 MHz para 13 C. Os desloc...

show abstract

“…Edema was induced in mice by applying 20 µL of croton oil solution (10 µg/µL) dissolved in 7:3 acetone/ water, to the inner surface of both ears, according to Van Arman (1974). A solution (20 µL) of the extract and fractions (0.25 mg/µL), or of indomethacin (0.05 mg/µL) was applied to the inner surface of the left ear.…”

Section: Anti-inflammatory Assaymentioning

confidence: 99%

Free radical scavenging and anti-edematogenic activities of Paullinia elegans Cambess., Sapindaceae, leaves extracts

Guimarães¹,

Truitti²,

Bersani-Amado³

et al. 2010

Rev. bras. farmacogn.

View full text Add to dashboard Cite

Anti‐inflammatory drugs

Cited by 59 publications

References 0 publications

Inhibition by prostaglandins of leukotriene B4 release from activated neutrophils.

Inhibition by prostaglandins of leukotriene B4 release from activated neutrophils.

CHEMICAL CONSTITUENTS AND EVALUATION OF ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF ROOTS OFSabicea brasiliensisWernh (Rubiaceae).

Free radical scavenging and anti-edematogenic activities of Paullinia elegans Cambess., Sapindaceae, leaves extracts

Contact Info

Product

Resources

About