Anti-inflammatory Constituents of the Red Alga <i>Gracilaria verrucosa</i> and Their Synthetic Analogues

Lee, Hye Ja; Yoo, Eun Sook; Shinde, Pramod B.; Lee, Yoon Mi; Hong, Jongki; Kim, Dong Kyoo; Jung, Jee H.

doi:10.1021/np070452q

Cited by 76 publications

(59 citation statements)

References 56 publications

(183 reference statements)

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“…Carrageenan was shown to stimulate the generation of superoxide, PMNs, and the secretion of TNF-α from macrophages (Shibata et al 2000). Similarly, there are reports about constituents of the red alga Gracillaria verrucosa that indicate its anti-inflammatory activity determined by their inhibitory effects on the production of the same pro-inflammatory mediators (NO, IL-6, and TNF-α; Dang et al 2008). Our results, however, do not agree with this observation; in fact, the polysaccharides from Hy.…”

Section: Discussionmentioning

confidence: 96%

Characterization of polysaccharides from Hypnea spinella (Gigartinales) and Halopithys incurva (Ceramiales) and their effect on RAW 264.7 macrophage activity

Abdala-Díaz

Chabrillón²,

Cabello‐Pasini³

et al. 2010

J Appl Phycol

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Red algae have been reported to be an important source of polysaccharides with potential immunomodulatory properties. The objective of this study was to characterize the polysaccharides from Halopithys incurva and Hypnea spinella and to evaluate their effect on the synthesis of cytokines by murine cell line RAW 264.7 macrophages. Polysaccharides were obtained by N-cetylpyridinium bromide precipitation and characterized by Fourier transform-infrared spectroscopy. Their effect on the activity of RAW 264.7 macrophages was examined by quantification of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and nitric oxide (NO) production using enzyme-linked immunosorbent assays. The activation of the cytokine IL-6 and NO increased linearly as the concentration of polysaccharides from H. incurva and Hy. spinella increased. In general, the activation of IL-6 and NO was tenfold greater when macrophages were exposed to polysaccharides from H. incurva than when exposed to polysaccharides from Hy. spinella. In contrast, TNF-α concentration did not increase when macrophages were exposed to increasing polysaccharide levels. These results indicate that polysaccharides are strong cytokine IL-6 inducers.

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Section: Discussionmentioning

confidence: 96%

Characterization of polysaccharides from Hypnea spinella (Gigartinales) and Halopithys incurva (Ceramiales) and their effect on RAW 264.7 macrophage activity

Abdala-Díaz

Chabrillón²,

Cabello‐Pasini³

et al. 2010

J Appl Phycol

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“…We previously showed that depleting activated macrophages and TAMs can reduce angiogenesis or lymphangiogenesis and tumor growth, which is closely associated with inflammatory stimuli (10)(11)(12)33), suggesting that natural anti-inflammatory compounds can suppress not only inflammatory signaling but also VEGFinduced angiogenesis. Dang et al developed anti-inflammatory enone fatty acids, including C10, by screening for the inhibition of NO, TNF-· and IL-6 production in stimulated macrophages (13). The enone fatty acid induced apoptosis and inhibited DNA synthesis in human cancer cells (34).…”

Section: Discussionmentioning

confidence: 99%

“…Dang et al isolated anti-inflammatory agents from red alga for the development of novel therapeutic drugs for inflammatory diseases (13). They showed that new prostaglandins and oxygenated fatty acids isolated from the red alga, Gracilaria verrucosa, inhibited the production of major mediators of inflammation, including nitric oxide (NO), IL-6 and TNF-·, in lipopolysaccharide (LPS)-activated RAW264.7 cells (13).…”

Section: Introductionmentioning

confidence: 99%

“…They showed that new prostaglandins and oxygenated fatty acids isolated from the red alga, Gracilaria verrucosa, inhibited the production of major mediators of inflammation, including nitric oxide (NO), IL-6 and TNF-·, in lipopolysaccharide (LPS)-activated RAW264.7 cells (13). In the present study, we investigated whether one of the anti-inflammatory agents extracted from the red alga, (E)-9-oxooctadec-10-enoic acid (C10), could inhibit angiogenesis in vitro and in vivo, and which inflammation-induced signaling pathways were involved in this anti-angiogenic activity.…”

Section: Introductionmentioning

confidence: 99%

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A natural anti-inflammatory enone fatty acid inhibits angiogenesis by attenuating nuclear factor-ÎºB signaling in vascular endothelial cells

et al. 2011

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Abstract. An anti-inflammatory enone fatty acid, (E)-9-oxooctadec-10-enoic acid (C10), was previously isolated from red alga (Gracilaria verrucosa). Of the many cellular signaling pathways activated in response to the inflammatory stimulus, lipopolysaccharide, the extracellular signalregulated kinase 1/2, the stress-activated protein kinase/Jun N-terminal kinase and the nuclear factor-κB pathways were specifically blocked by C10 in the macrophage-like cell line, RAW264.7. In this study, we investigated the anti-angiogenic and anti-inflammatory activities of C10 in endothelial cells. C10 only partially inhibited the proliferation of human cancer cell lines at relatively high concentrations of over 20 μg/ml. However, C10 inhibited the proliferation of RAW264.7 cells and human umbilical vein endothelial cells (HUVECs) with half-maximal inhibitory concentration (IC 50 ) values of 4-8 μg/ml. Both the proliferation and the migration of HUVECs induced by the vascular endothelial growth factor (VEGF) were markedly blocked by C10 with IC 50 values of 2-3 μg/ml. The activation of nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, ·, by tumor necrosis factor-· or VEGF in these cells was also blocked by C10. Furthermore, in an in vivo model of angiogenesis in the mouse cornea, the neovascularization induced by VEGF was markedly inhibited by C10. The processes involved in inflammatory signaling, angiogenesis, and the development of malignancy in cancer are closely related, suggesting that C10 could be a useful lead compound for the development of novel anti-angiogenic therapies for cancer.

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“…A wide range of antibiotic, anti-HIV, anticoagulant, anticonvulsant, anti-inflammatory (Dang et al, 2008), antineoplastic, wound healing, antiulcer (Nagaoka et al, 2000), hepatoprotective (Mosaad et al, 2006) and antitumor activities for seaweed derived compounds have been reported (Lincoln et al, 1991). Seaweeds also assumed greater significance as they are rich sources of various types of antioxidant compounds (Kuda et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Antiulcer, wound healing and hepatoprotective activities of the seaweeds Gracilaria crassa, Turbinaria ornata and Laurencia papillosa from the southeast coast of India

Senthil¹,

Murugan²

2013

Braz. J. Pharm. Sci.

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Seaweeds have bioactive compounds of interest in the pharmaceutical industry. In India, seaweeds are used exclusively for phycocolloids production and have not yet received consideration as a dietary supplement. So, it has become imperative to explore the biomedical potential of seaweeds and promote their utilization as a functional food. The seaweeds Turbinaria ornata, Gracillaria crassa and Laurencia papillosa, collected from the Tuticorin coast of the Southeast coast of India and selected based on preliminary screening, were extracted with acetone and evaluated for antiulcer, wound healing and hepatoprotective activities. L. papillosa showed the highest level of gastric protection activity (81%) at 200 mg/kg, comparable to the standard drug ranitidine (90%). G. crassa followed with 76%. G. crassa and L. papillosa, showed marked wound-healing activity. G. crassa at 200 mg/kg, showed a marked effect on the serum marker enzymes indicating prominent hepatoprotective activity. The noteworthy wound-healing and hepato-protective properties of G. crassa besides anti-ulcer activity next to L. papillosa were indicative of its potential for further consideration.Uniterms: Seaweed/biomedical potential. Seaweed/antiulcer activity. Seaweed/wound-healing. Seaweed/ hepatoprotective activity. Gracilaria crassa/biomedical potential. Turbinaria ornata/biomedical potential. Laurencia papillosa/biomedical potential.Algas marinhas possuem compostos bioativos de interesse para a indústria farmacêutica. Na Índia, as algas marinhas são usadas exclusivamente para a produção de ficocolóides e ainda não receberam a consideração como um suplemento dietético. Assim, tornou-se imperativo explorar o potencial biomédico de algas e promover a sua utilização como alimento funcional. As algas marinhas Turbinaria ornata, Gracillaria crassa e Laurencia papillosa, coletadas de Tuticorin, na costa sudeste da Índia, e selecionadas com base em triagem preliminar, foram extraídas com acetona e avaliadas quanto à atividade antiúlcera, de cicatrização de feridas e de hepatoproteção. L. papillosa mostrou o mais alto nível da atividade de proteção gástrica (81%) na dose de 200 mg/kg, comparável ao fármaco padrão, ranitidina (90%). G. crassa mostrou atividade de 76%. G. crassa e L. papillosa mostrou atividade martcante na cicatrização de feridas. G. Crassa, a 200 mg/kg, mostrou efeito alto sobre o marcador sérico das enzimas, indicando atividade hepatoprotetora proeminente. A notável cura de feridas e as propriedades hepatoprotetoras de G. Crassa, além da atividade antiúlcera, próxima da L. Papillosa, foram indicativos do seu potencial para uma análise mais aprofundada.

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Anti-inflammatory Constituents of the Red Alga Gracilaria verrucosa and Their Synthetic Analogues

Cited by 76 publications

References 56 publications

Characterization of polysaccharides from Hypnea spinella (Gigartinales) and Halopithys incurva (Ceramiales) and their effect on RAW 264.7 macrophage activity

Characterization of polysaccharides from Hypnea spinella (Gigartinales) and Halopithys incurva (Ceramiales) and their effect on RAW 264.7 macrophage activity

A natural anti-inflammatory enone fatty acid inhibits angiogenesis by attenuating nuclear factor-ÎºB signaling in vascular endothelial cells

Antiulcer, wound healing and hepatoprotective activities of the seaweeds Gracilaria crassa, Turbinaria ornata and Laurencia papillosa from the southeast coast of India

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