Anti-inflammatory and lysosomal stability actions of Cleome gynandra L. studied in adjuvant induced arthritic rats

Narendhirakannan, R. T.; Subramanian, S.; Kandaswamy, M.

doi:10.1016/j.fct.2006.12.009

Cited by 61 publications

(30 citation statements)

References 36 publications

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“…The uncontrolled production of ROS (especially superoxide anion and hydroxyl radicals) by phagocytic cells due to the inflammatory surge in RA leads to a decrease in SOD and CAT activities in addition to GSH level as a consequence of their supersaturation and consumption during oxidative stress as well as loss through cellular lysis, which resulted from membrane lipid peroxidation and lysosomal destruction [43,44]. The significant increase in serum ALAT and ALP activities as well as liver LPO level (sensitive markers of cellular integrity and toxicity) of arthritic rats in the present study are in agreement with the reported significant decrease in lysosomal stability of CFA treated rats [21]. Elevated level of serum ALP activity in arthritic rats can also be due to the increase in bone erosion and periarticular ostopenia, since the enzyme is released into circulation in the course of bone resorbtion and formation [45].…”

Section: Discussionsupporting

confidence: 89%

“…Arthritis was induced by a single intra-dermal injection of 0.1 ml of CFA into the palmar surface of the left hind paw after the rats were subjected to light diethyl ether anaesthesia [21]. Arthritic rats received orally (by gavage) and daily (for 28 consecutive days) 1.0 ml distilled water as vehicle (arthritis control group), indomethacin (1.0 mg/kg body weight), or 200 mg/kg body weight of either turmeric or ginger rhizomes powder suspended in 1.0 ml distilled water (TRPS or GRPS, respectively) from the day of arthritis induction (day 0).…”

Section: Experimental Design and Treatment Schedulementioning

confidence: 99%

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Anti-inflammatory and Anti-oxidant Properties of Curcuma longa (Turmeric) Versus Zingiber officinale (Ginger) Rhizomes in Rat Adjuvant-Induced Arthritis

2010

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Turmeric (rich in curcuminoids) and ginger (rich in gingerols and shogaols) rhizomes have been widely used as dietary spices and to treat different diseases in Ayurveda/Chinese medicine since antiquity. Here, we compared the anti-inflammatory/anti-oxidant activity of these two plants in rat adjuvant-induced arthritis (AIA). Both plants (at dose 200 mg/kg body weight) significantly suppressed (but with different degrees) the incidence and severity of arthritis by increasing/decreasing the production of anti-inflammatory/pro-inflammatory cytokines, respectively, and activating the anti-oxidant defence system. The anti-arthritic activity of turmeric exceeded that of ginger and indomethacin (a non-steroidal anti-inflammatory drug), especially when the treatment started from the day of arthritis induction. The percentage of disease recovery was 4.6-8.3% and 10.2% more in turmeric compared with ginger and indomethacin (P < 0.05), respectively. The present study proves the anti-inflammatory/anti-oxidant activity of turmeric over ginger and indomethacin, which may have beneficial effects against rheumatoid arthritis onset/progression as shown in AIA rat model.

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Section: Discussionsupporting

confidence: 89%

Section: Experimental Design and Treatment Schedulementioning

confidence: 99%

Anti-inflammatory and Anti-oxidant Properties of Curcuma longa (Turmeric) Versus Zingiber officinale (Ginger) Rhizomes in Rat Adjuvant-Induced Arthritis

2010

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“…1. The previous studies showed that ethyl acetate extract of C. gynandra found to possess antioxidant, anti-inflammatory, and lysosomal protection properties in adjuvant induced arthritic rats [22]. The antioxidant property of biomolecules, in particular, the plant extract is determined by its ability to scavenge DPPH radicals [23].…”

Section: Resultsmentioning

confidence: 99%

Gas Chromatography-Mass Spectrometry Analysis, in Vitro Cytotoxic and Antioxidant Efficacy Studies on Cleome Gynandra L. (Leaves): A Traditional Drug Source

Saravanan

Brindha

Narayanan

et al. 2017

Asian J Pharm Clin Res

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Objective: This study was aimed to assess the phytoconstituents, cytotoxic, and antioxidant efficacy of ethyl extract of Cleome gynandra leaves.Methods: Qualitative phytochemical analysis with different solvent extracts was performed. Quantitative and gas chromatography-mass spectrometry (GC-MS) analysis of the extract was performed with ethyl acetate extract. The cytotoxic effect of the ethyl acetate extract was determined by 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide (MTT) assay on Michigan Cancer Foundation-7 (MCF-7) cells using taxol as standard and free radical scavenging ability using 1,1-diphenyl-2-picrylhydrazyl (DPPH). Results:Leaves extracts with different solvents revealed the presence of alkaloids, cardiac glycosides, flavonoids, phenols, and tannins. GC-MS analysis of ethyl acetate of the plant leaves showed the presence of n-hexadecanoic acid. The IC 50 value of the ethyl acetate extract was found to be 90.2 μg/ml on MCF-7 cell line, and the extract was found to possess significant DPPH free radical scavenging activity. Conclusion:From the results, we conclude that the C. gynandra extract possesses antioxidant and antiproliferating activity against MCF-7 cells.

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“…Drugs capable of stabilizing the lysosomal membrane can prevent its rupture and reduce the degradation of connective tissue consecutively reducing the disease severity. In adjuvant induced arthritic rat the releases of glycohydrolases are the important index for the examination of integrity of the lysosomal membrane and are responsible for the tissue damage (Narendhirakannan et al 2007). In our study, there was a marked increase in the activity of lysosomal enzymes in both plasma and liver of adjuvant induced arthritic rats.…”

Section: Discussionmentioning

confidence: 99%

“…These enzymes are involved in the degradation of structural molecules in connective tissue and cartilage proteoglycans. In adjuvant induced arthritis, the development of inflammatory process is correlated with increased release of lysosomal enzymes in the extracellular compartment (Narendhirakannan et al 2007). The changes in the activities of lysosomal enzymes, glycoproteins and collagen metabolism manifest the systemic damage produced during adjuvant arthritis (Rasool and Varalakshmi 2007).…”

Section: Introductionmentioning

confidence: 99%

Therapeutic effect of Saraca asoca (Roxb.) Wilde on lysosomal enzymes and collagen metabolism in adjuvant induced arthritis

Saravanan¹,

Narasimhan²,

Pandikumar³

et al. 2011

Inflammopharmacol

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Rheumatoid arthritis is a chronic, progressive and systemic inflammatory disorder mainly affecting the synovial joints. In the present study, we evaluated the anti-arthritic effect of the methanol extract of Saraca asoca (Roxb.) Wilde., (Fabaceae) on adjuvant induced arthritis by assessing paw swelling, body weight, the levels of lysosomal enzymes, protein bound carbohydrates, serum cytokines, urinary collagen and histopathology of joints. It was found that S. asoca methanol extract at doses of 50, 100 and 200 mg/kg reduced the paw thickness and elevated the mean body weight of arthritic rats. The treatment of S. asoca showed a significant reduction in the levels of both plasma and liver lysosomal enzymes. The protein bound carbohydrates and urinary collagen contents were also decreased at a significant level by the treatment of S. asoca methanol extract. The histopathological study of the joints showed the anti-arthritic property of S. asoca which nearly normalized the histological architecture of the joints. Further, we established the anti-arthritic activity of S. asoca methanol extract by measuring the levels of cytokines in both arthritic and treated rats. The treatment of S. asoca reduced the levels of pro-inflammatory cytokines. In conclusion, S. asoca methanol extract was capable of ameliorating the conditions of arthritis in adjuvant induced arthritic rats.

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Anti-inflammatory and lysosomal stability actions of Cleome gynandra L. studied in adjuvant induced arthritic rats

Cited by 61 publications

References 36 publications

Anti-inflammatory and Anti-oxidant Properties of Curcuma longa (Turmeric) Versus Zingiber officinale (Ginger) Rhizomes in Rat Adjuvant-Induced Arthritis

Anti-inflammatory and Anti-oxidant Properties of Curcuma longa (Turmeric) Versus Zingiber officinale (Ginger) Rhizomes in Rat Adjuvant-Induced Arthritis

Gas Chromatography-Mass Spectrometry Analysis, in Vitro Cytotoxic and Antioxidant Efficacy Studies on Cleome Gynandra L. (Leaves): A Traditional Drug Source

Therapeutic effect of Saraca asoca (Roxb.) Wilde on lysosomal enzymes and collagen metabolism in adjuvant induced arthritis

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