Anti‐Inflammatory and Antioxidant Activities of <i>Salvia fruticosa</i>: An HPLC Determination of Phenolic Contents

Boukhary, Rima; Raafat, Karim; Ghoneim, Asser I.; Aboul-Ela, Maha; El-Lakany, Abdalla

doi:10.1155/2016/7178105

Cited by 46 publications

(47 citation statements)

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“…Up until now, different Salvia species have been investigated for their biological activity including anti-inflammatory [12], antioxidant-antifungal [13], anticancer [14], as well as antiviral activities [15]. A promising study also suggested that Salvia lavandulifolia Vahl.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, rosmarinic acid derivatives from S. officinalis showed potent antioxidant activity greater than a standard synthetic antioxidant, trolox [27]. Moreover, S. fruticosa , which is rich in luteolin and rutin, showed significant anti-inflammatory activity comparable to a standard drug [12]. Therefore, it is interesting to discover the constituents and biological activities of different Salvia species due to their pharmacological potential.…”

Section: Introductionmentioning

confidence: 99%

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RP-HPLC/MS/MS Analysis of the Phenolic Compounds, Antioxidant and Antimicrobial Activities of Salvia L. Species

et al. 2016

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The identification and quantification of the phenolic contents of methanolic extracts of three Salvia L. species namely S. brachyantha (Bordz.) Pobed, S. aethiopis L., and S. microstegia Boiss. and Bal. were evaluated using reverse phase high performance liquid chromatography, UV adsorption, and mass spectrometry (RP-HPLC/MS). In order to determine the antioxidant capacity of these species, cupric ions (Cu2+) reducing assay (CUPRAC) and ferric ions (Fe3+) reducing assay (FRAP) were performed to screen the reducing capacity and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay was employed for evaluation of the radical scavenging activity for both solvents. In further investigation, the antimicrobial activities of Salvia species were tested using the disc diffusion method against three Gram-positive and four Gram-negative microbial species, as well as three fungi species. The results showed that there is a total of 18 detectable phenols, the most abundant of which was kaempferol in S. microstegia and rosmarinic acids in S. brachyantha and S aethiopis. The other major phenols were found to be apigenin, luteolin, p-coumaric acid, and chlorogenic acid. All species tested showed moderate and lower antioxidant activity than standard antioxidants such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), and ascorbic acid. The ethanolic extracts of Salvia species revealed a wide range of antimicrobial activity. S. brachyantha and S. microstegia showed the highest antimicrobial activities against B. subtilis, whereas S. aethiopis was more effective on Y. lipolytica. None of the extracts showed anti-fungal activity against S. cerevisiae. Thus these species could be valuable due to their bioactive compounds.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

RP-HPLC/MS/MS Analysis of the Phenolic Compounds, Antioxidant and Antimicrobial Activities of Salvia L. Species

et al. 2016

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“…Referring to literature, it was found that, all the observed spectral data are similar to those reported for dehydro-abietic acid. 20,21 Also, it is isolated for the first time from Salvia fruticosa libanotica ( Figure 1 and Table 2). …”

Section: Extraction and Isolationmentioning

confidence: 96%

Chemical Constituents from Salvia fruticosa libanotica

Boukhary¹,

Aboul-Ela²,

Al-Hanbali³

et al. 2017

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Objective: Plants of genus Salvia are used in traditional folk medicine for their antidiabetic, anti-oxidant effects and for gastric disorders. The oil has an antiseptic property and it is used as a fragrance in soaps and perfumes. An infusion of the leaves is widely used as a mouthwash or gargle and for the treatment of inflammation of the mouth and throat. It is used as carminative, antiinflammatory, astringent, cytotoxic and antispasmodic. Materials and Methods: Air-dried powdered aerial parts and roots of Salvia fruticosa libanotica were extracted separately with ethanol and acetone then their residues were separately extracted with different solvents on silica gel columns. Results: From Salvia fruticosa libanotica, eight phenolic compounds were isolated from methylene chloride, ethyl acetate and butanol extracts of the chosen plant. The chemical structures of the isolated compounds were determined by NMR, MS, IR and UV spectroscopic methods. Conclusion: Among these compounds three Flavonoids named apigenin, luteolin and rutin were identified in addition to three phenolic acids which are ferulic acid, gallic acid and rosemarinic acid from aerial parts. Moreover, carnosol and dehydro-abietic acid were also isolated from Salvia fruticosa libanotica root extract. All the isolated compounds were obtained for the first time from Salvia fruticosa libanotica. Keywords: Salvia fruticosa libanotica, Antidiabetic, Anti-oxidant, Rutin; Carnosol, Dehydroabietic acid. Therefore, this research aims to isolate the antioxidative phenolic constituents including flavonoids and abietane diterpenoids from both aerial and root parts that might have biological activities. Rima MATERIALS AND METHODS GeneralThe IR spectra were determined on Shimadzu IR spectrophotometer (FT/IR-8300) in KBr discs and the absorption bands were measured in cm CNMR spectra were recorded on Bruker Avance 500MHZ apparatus. Column chroma tography was performed over silica gel (70-230, mesh, Fluka) using petroleum ether, methylene chloride, ethyl acetate and methanol gradients as eluents. UV spectra were determined using CibaCorning Double-beam spectrophotometer (2800 spectroscan) and mass spectra were recorded on a AEIMS-50 spectrometer. 6.25 (1H, d, J=2Hz, 6.4 (1H, d, J=2Hz, 7.6 (1H, s, 6.82 (1H, d, J=8Hz, 7.52 (1H, dd, J=8.2Hz,2Hz, 5.15 (1H, d, J=2Hz, 4.54 (4H, d, J=8Hz, d, J=1Hz,. Plant material 13CNMR (DMSO,500 MHz); 158.5 (C-2), 134 (C-3), 178 (C-4), 162.5 (C-5), 101.5 (C-6), 164 (C-7), 93.8 (C-8), 123.1 (C-1'), 117.5 (C-2'), 144 (C-3'), 148.5 (C-4'), 116.5 (C-5') and 123.5 (C-6'). RESULTS AND DISCUSSIONCompounds 1, 2 and 3 were found to be 5-hydroxyflavonol derivatives. Compound 1 was deduced to be C 15 H 11 O 5 and its UV spectra in AlCl 3 and AlCl 3 /HCl showed the absence of 3'-OH. EIMS showed its molecular ion peak at m/z=270. 1 HNMR was integrated for 11 protons. Accordingly, compound 1 was identified as apigenin. It was confirmed by co-chromatography with apigenin standard. The molecular formula of compound 2 was deduced to be C 1...

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“…Different plant extracts demonstrated strong radical scavenging activity, whereas the ethyl acetate extract had the highest value in the roots and the lowest in the aerial parts. This antioxidant activity was correlated with the total phenolic content of different extracts, where rutin and luteolin were the most abundant constituents [22].…”

Section: Sage Extractsmentioning

confidence: 97%

Current State of the Art on the Antioxidant Activity of Sage (Salvia spp.) and Its Bioactive Components

2020

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Common sage (Salvia spp., with the most common species Salvia officinalis) is an important medicinal and aromatic plant due to its bioactive components, secondary products of its metabolism. These components are mainly phenolics, terpenoids, polyphenols, and flavonoids. Many studies have identified their important role in fighting oxidative stress in cells and organisms, together with their anticancer, antimicrobial, and anti-inflammatory role. There are many methods measuring the antioxidant activity of sage phenolic components, usually based on radical scavenging of free radical species, such as 2,2-diphenyl-1-picrylydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and determining the absorbance of the reduced product by a photometric assay. In addition, there are several in vitro and in vivo studies determining the protection of sage extracts in cells in culture or animals, respectively, after induction of oxidative stress. In this review, results from the currently available studies that unravel the significant role of sage bioactive compounds, as antioxidant compounds, and the variety of methods used have been critically analyzed and discussed.

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Anti‐Inflammatory and Antioxidant Activities of Salvia fruticosa: An HPLC Determination of Phenolic Contents

Cited by 46 publications

References 19 publications

RP-HPLC/MS/MS Analysis of the Phenolic Compounds, Antioxidant and Antimicrobial Activities of Salvia L. Species

RP-HPLC/MS/MS Analysis of the Phenolic Compounds, Antioxidant and Antimicrobial Activities of Salvia L. Species

Chemical Constituents from Salvia fruticosa libanotica

Current State of the Art on the Antioxidant Activity of Sage (Salvia spp.) and Its Bioactive Components

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