Anti‐inflammatory and anticancer activities of Naringenin‐loaded liquid crystalline nanoparticles in vitro

Abstract

In this study, we had developed Naringenin-loaded liquid crystalline nanoparticles (LCNs) and investigated the anti-inflammatory and anticancer activities of Naringenin-LCNs against human airway epithelium-derived basal cells (BCi-NS1.1) and human lung epithelial carcinoma (A549) cell lines, respectively. The anti-inflammatory potential of Naringenin-LCNs evaluated by qPCR revealed a decreased expression of IL-6, IL-8, IL-1β, and TNF-α in lipopolysaccharide-induced BCi-NS1.1 cells. The activity of LCNs was comparable to the positive control drug Fluticasone propionate (10 nM). The anticancer activity was studied by evaluating the antiproliferative (MTT and trypan blue assays), antimigratory (scratch wound healing assay, modified Boyden chamber assay, and immunoblot), and anticolony formation activity in A549 cells. Naringenin LCNs showed promising antiproliferative, antimigratory, and anticolony formation activities in A549 cells, in vitro. Therefore, based on our observations and results, we conclude that Naringenin-LCNs may be employed as a potential therapy-based intervention to ameliorate airway inflammation and to inhibit the progression of lung cancer. Practical applications Naringenin was encapsulated into liquid crystalline nanoparticles, thus, attributing to their sustained-release nature. In addition, Naringenin-loaded LCNs efficiently reduced the levels of pro-inflammatory markers, namely, IL-1β, IL-6, TNF-α, and IL-8. In addition, the Naringenin-loaded LCNs also possess potent anticancer activity, when tested in the A549 cell line, as revealed by the inhibition of proliferation and migration of cells. They also attenuated colony formation and induced apoptosis in the A549 cells. The findings from our study could form the basis for future research that may be translated into an in vivo model to validate the possible therapeutic alternative for lung cancer using Naringenin-loaded LCNs. In addition, the applications of

Berberine loaded liquid crystalline nanostructure inhibits cancer progression in adenocarcinomic human alveolar basal epithelial cells in vitro

“…The physicochemical characterization/parameters and in vitro drug release of berberine LCNs were found in agreement with our previous studies involving similar LCNs encapsulating other phytoceuticals, rutin, and naringenin. Moreover, we have also observed significant in vitro anticancer activity with those LCNs formulation (Paudel et al., 2021; Wadhwa et al., 2021). This overlay of the findings amongst various phytoceuticals berberine, rutin and naringenin clearly highlights the validity and application of LCNs in lung cancer.…”

“…Berberine loaded liquid crystalline nanoparticles was formulated as previously mentioned (Wadhwa et al., 2021). Berberine‐LCNs were prepared using ultrasonication method.…”

“…Liquid crystalline nanoparticles (LCNs) received a lot of attention in the drug delivery domain due to their exceptional capacity in improving drug bioavailability, stability, minimize toxicity, and modulating release of drug after administration (Madheswaran et al., 2019). Furthermore, LCNs have been proven to be effective in delivering several herbal moieties as well as berberine against cancer disorder (Angelova et al., 2017; Chen et al., 2015; Li et al., 2019; Loo et al., 2020; Wadhwa et al., 2021; Zou et al., 2017). Considering the significant role of IL‐8, CCL‐20, and HO‐1 in cancer progression, our current study aimed to investigate the potential of berberine loaded liquid crystalline nanoparticles (berberine‐LCNs) in inhibiting inflammation‐mediated cancer progression in the lung cancer (A549) cell line in vitro.…”

An Evaluation on Use of Stress Ball Exercise on Stress Management among Student Population – A Cross Section Study

“…Acute stress is short term stress which goes away quickly. Our team has extensive knowledge and research experience that has translate into high quality publications [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. Doing something new may cause acute stress.…”

Estimation of Tactile Sensation by Two Point Discrimination among 18 Years Old People

“…There are several factors which influence two point discrimination tests like age, sex, devices, applied force and also the state of mind of participants [17]. All these factors affect the normative values of two point discrimination test [18]. The two point discrimination can also be hypothesised as the value of tactile sensation is not more in 18 years old people [19].…”