Anti-inflammatory and anti-angiogenic activities of a purified polysaccharide from flesh of Cipangopaludina chinensis

Xiong, Qingping; Hao, Hairong; He, Lian; Jing, Yi; Xu, Tingting; Chen, Jing; Zhang, Haijiang; Hu, Tao; Zhang, Qianghua; Yang, Xue; Yuan, Jun; Huang, Yu

doi:10.1016/j.carbpol.2017.08.073

Cited by 45 publications

(18 citation statements)

References 28 publications

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“…The proliferation, chemotaxis, migration, and lumen formation of vascular endothelial cells are the initial and central links of angiogenesis [ 10 ]. Based on this spot, the evaluation of angiogenesis in vitro is mainly simulated by experiments on proliferation, migration, and lumen formation of HUVECs at present [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…The content of the sulphated group in polysaccharides was tested by the barium chloride-gelatin method using K 2 SO 4 as the standard [ 21 ]. The sulfuric acid-m-hydroxyl biphenyl method was hired to evaluate the uronic acid content of this polysaccharide [ 10 ].…”

Section: Methodsmentioning

confidence: 99%

“…After the completion of the incubation, the cells were washed with PBS and then treated with MTT kits according to the manufacturer's guidelines. The cell proliferation activity of APBOM on HUVECs was evaluated by its cell viability (%), which was calculated with the reported method [ 10 ].…”

Section: Methodsmentioning

confidence: 99%

“…The inhibitory effect of APBOM on HUVECs tube formation was tested by Matrigel-based tube formation experiments. The assays were performed as the reported method [ 10 , 23 ].…”

Section: Methodsmentioning

confidence: 99%

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Extraction, Purification, Characterization, and Antiangiogenic Activity of Acidic Polysaccharide from Buddleja officinalis

Yan

Xiong

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Firstly, optimal parameters of crude polysaccharide from Buddleja officinalis were obtained as follows: ratio of water to raw material of 26 : 1, ultrasonic power of 240 W, ultrasonic time of 45 min, and ultrasonic temperature of 62°C. Secondly, acidic polysaccharide (APBOM) from Buddleja officinalis was successfully acquired with the yield of 9.57% by using DEAE-52 cellulose and Sephadex G-100 gel column chromatography. Then, we found that total polysaccharide content of APBOM was 94.37% with a sulfuric acid group of 1.68%, uronic acid content of 17.41%, and average molecular weight of 165.4 kDa. Finally, APBOM was confirmed to have significant antiangiogenic effects.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The inhibitory effect of APBOM on HUVECs tube formation was tested by Matrigel-based tube formation experiments. The assays were performed as the reported method [ 10 , 23 ].…”

Section: Methodsmentioning

confidence: 99%

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Extraction, Purification, Characterization, and Antiangiogenic Activity of Acidic Polysaccharide from Buddleja officinalis

Yan

Xiong

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…In particular, G. biloba sarcotesta polysaccharides display good antitumor activities on human stomach cancer cells AGS and human leukemia cells (U937) . Polysaccharides from natural sources have been reported to be effective in anti‐inflammatory processes . LPS‐induced RAW 264.7 macrophages, which are widely used as a model for studying inflammatory responses, were used in this study to evaluate the potential anti‐inflammatory effects of GBSP3a.…”

Section: Discussionmentioning

confidence: 99%

Ginkgo biloba sarcotesta polysaccharide inhibits inflammatory responses through suppressing both NF‐κB and MAPK signaling pathway

Huang

et al. 2018

J Sci Food Agric

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BACKGROUND: Polysaccharides, common components of natural products extensively studied as dietary supplements and functional foods, have been found to have various activities. In the present study, a water-soluble polysaccharide, namely GBSP3a, was isolated and purified from G. biloba sarcotesta. The anti-inflammatory activity of GBSP3a in lipopolysaccharide (LPS)-induced RAW264.7 macrophages and the potential underlying molecular mechanisms were then assessed. RESULTS: GBSP3a exerted its anti-inflammatory effect by remarkably inhibiting the secretion of pro-inflammatory mediators and cytokines, including nitric oxide (NO), prostaglandin E 2 (PGE 2 ), tumor necrosis factor-(TNF-), interleukin-6 (IL-6) and interleukin-1 (IL-1 ) in LPS-stimulated RAW264.7 macrophages. Excessive mRNA and protein expression levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were dose-dependently inhibited by GBSP3a in LPS-stimulated RAW264.7 cells. Further research suggested that the anti-inflammatory effect of GBSP3a can be attributed to the modulation of the NF-B and MAPK signaling pathways. CONCLUSION: GBSP3a exhibits anti-inflammatory activity and exerts its anti-inflammatory effect probably through suppressing both NF-B and MAPK signaling pathway, indicating that GBSP3a could be used for the development of anti-inflammatory agent or nutraceuticals. Statistical analysisAll results were from at least triplicate experiments and are presented as the mean ± standard deviation (SD). Statistical analysis was conducted using the SPSS 13.0 software (IBM Corp., Armonk, NY, USA). Analysis of significant difference was assessed by one-way analysis of variance (ANOVA), followed by the Duncan's multiple-range test. Statistical significance was set as P < 0.05. RESULTS Homogeneity, molecular weight and monosaccharide composition of GBSP3aAfter purification by gel filtration chromatography, GBSP3a was obtained as an off-white powder. It had no absorption at 280 or 260 nm in the UV spectrum and had a negative response to the Folin-phenol test and barium chloride test, indicating the absence of protein, nucleic acid and sulfated groups. The content of uronic acid in GBSP3a was 7.92% as detected by the meta-hydroxydiphenyl colorimetric method. 35 The HPGPC profile of GBSP3a (Fig. 1A) displays a single and symmetrical peak, indicating that GPSB3a is a homogeneous polysaccharide. The weight average molecular weight of GBSP3a, as determined by high-performance size exclusion chromatography, was J Sci Food Agric 2019; 99: 2329-2339 CONCLUSIONIn the present study, a water-soluble polysaccharide called GBSP3a was isolated from G. biloba sarcotesta. The anti-inflammatory activity of GBSP3a was then assessed in LPS-induced RAW264.7 macrophages. The results indicated that GBSP3a exerted its J Sci Food Agric 2019; 99: 2329-2339

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Chemical Composition and Potential Antimicrobial and Anti‐Inflammatory Activities of Essential Oil from Fruits of Alpinia zerumbet (Pers.) B.L.Burtt & R.M.Sm.

Hou,

Gong,

Gong

et al. 2023

Chemistry & Biodiversity

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Alpinia zerumbet (Pers.) B.L.Burtt & R.M.Sm. was extensively used in traditional medicine for its several properties, but continuous investigation is needed to discover the properties of its essential oils (EOs). This work evaluated the properties of an EO obtained by steam distillation (named ESD) as well as extracts obtained by petroleum ether (named EP) both from Alpinia zerumbet fruits. Gas chromatography‐mass spectrometry (GC‐MS) was chosen to identify the composition, and eleven compounds were identified as the main components of the EO and EP of Alpinia zerumbet fruits. The antimicrobial properties were investigated by minimum inhibitory concentration (MIC) and the inhibition area. The results identified the differences in antimicrobial activities attributed to different extraction methods. Enzyme‐linked immunosorbent assay (ELISA) and Western Blot (WB) assay were conducted to assess the anti‐inflammatory effects of ESD. In conclusion, our study suggested that EO from Alpinia zerumbet fruits might be a prospective candidate for antimicrobial and anti‐inflammatory therapy.

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Anti-inflammatory and anti-angiogenic activities of a purified polysaccharide from flesh of Cipangopaludina chinensis

Cited by 45 publications

References 28 publications

Extraction, Purification, Characterization, and Antiangiogenic Activity of Acidic Polysaccharide from Buddleja officinalis

Extraction, Purification, Characterization, and Antiangiogenic Activity of Acidic Polysaccharide from Buddleja officinalis

Ginkgo biloba sarcotesta polysaccharide inhibits inflammatory responses through suppressing both NF‐κB and MAPK signaling pathway

Chemical Composition and Potential Antimicrobial and Anti‐Inflammatory Activities of Essential Oil from Fruits of Alpinia zerumbet (Pers.) B.L.Burtt & R.M.Sm.

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