Extraction, Purification, Characterization, and Antiangiogenic Activity of Acidic Polysaccharide from Buddleja officinalis

Abstract

Firstly, optimal parameters of crude polysaccharide from Buddleja officinalis were obtained as follows: ratio of water to raw material of 26 : 1, ultrasonic power of 240 W, ultrasonic time of 45 min, and ultrasonic temperature of 62°C. Secondly, acidic polysaccharide (APBOM) from Buddleja officinalis was successfully acquired with the yield of 9.57% by using DEAE-52 cellulose and Sephadex G-100 gel column chromatography. Then, we found that total polysaccharide content of APBOM was 94.37% with a sulfuric acid group of 1.68%, uronic acid content of 17.41%, and average molecular weight of 165.4 kDa. Finally, APBOM was confirmed to have significant antiangiogenic effects.

Acidic polysaccharides from Buddleja officinalis inhibit angiogenesis via the Nrf2/ARE pathway to attenuate diabetic retinopathy

“…The APBOM dose was selected according to cell viability, Transwell migration assay and Matrigel-based tube formation assay. 8 The HUVECs were divided into 5 groups and treated as follows: normal group (NG), high-glucose group (HG), 40 μg mL −1 APBOM group, 80 μg mL −1 APBOM group, and 160 μg mL −1 APBOM group. Aside from the NG (5.5 mmol L −1 glucose), other groups were cultured with 30 mmol L −1 glucose.…”

“…3 †) were used to determine the dose of APBOM. 8 Based on the cell viability results, 20, 40, 80 and 160 μg mL −1 APBOM were selected for subsequent experiments, which had no significant effect on the proliferation of HUVECs. After intervention with APBOM, compared with the control group, the migration rate of HUVECs significantly decreased to 87.56%, 68.81%, 47.99% and 27.17% respectively (ESI Fig.…”

“…6,7 In our previous research, acid polysaccharides from Buddleja officinalis Maxim (APBOM) were prepared and confirmed to have significant anti-angiogenic effects. 8 Therefore, we speculated that APBOM might intervene in the process of DR by inhibiting angiogenesis.…”

“…">Materials and reagentsAPBOM was prepared and characterized according to the previous report. 8 The UV-vis spectrum, HPGPC, FT-IR spectrum and monosaccharide composition analysis of APBOM are presented in ESI Fig. 1.…”