Anti-inflammatory and anti-allergic effects of Agrimonia pilosa Ledeb extract on murine cell lines and OVA-induced airway inflammation

Kim, Jae Jin; Jiang, Jun; Shim, Do-Wan; Kwon, Sang Chul; Kim, Tack Joong; Ye, Sang Kyu; Kim, Myong Ki; Shin, Yong Kook; Koppula, Sushruta; Kang, Tae-Bong; Choi, Dong‐Kug; Lee, Kwang Ho

doi:10.1016/j.jep.2011.12.035

Cited by 51 publications

(33 citation statements)

References 40 publications

(37 reference statements)

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“…Previous references (7,8) have reported that the methanol extract of AP inhibited the production of NO and PGE2 via the downregulation of iNOS and COX-2 expression in RAW 264.7 cells. The expression levels of proinflammatory cytokines IL-1β and IL-6 were inhibited by methanol extract of AP, but no effect was observed in TNF-α production.…”

Section: Discussionmentioning

confidence: 91%

“…As a traditional medicinal plant, it has been used for the treatment of abdominal pain, sore throat, headaches, bloody discharge, parasitic infections and eczema (1). Pharmacological research has revealed that AP extracts possess diverse pharmacological properties, including antinociceptive (1), antiplatelet (2) and antihemostatic effects (3), in addition to antioxidant (4), antiviral (5), antitumor (6), anti-inflammatory, anti-allergic (7,8), anti-microbial (9) and anti-hyperglycemic (10) activity, amongst others. Tiliroside, a glycosidic flavonoid ( Fig.…”

Section: Introductionmentioning

confidence: 99%

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Tiliroside, the major component of Agrimonia pilosa Ledeb ethanol extract, inhibits MAPK/JNK/p38-mediated inflammation in lipopolysaccharide-activated RAW 264.7 macrophages

Jin

Song

Wang

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. In the present study, the in vivo anti-inflammatory activity of Agrimonia pilosa Ledeb (AP) ethanol extract was confirmed in experimental animal models, including xylene-induced ear edema in mice and carrageenan-induced paw edema in rats. Tiliroside, the major component of AP extract, was isolated and purified by high-performance liquid chromatography. The anti-inflammatory mechanism of tiliroside was then examined using lipopolysaccharide (LPS)-activated RAW 264.7 macrophage cells. An MTT assay was used to determine cytotoxicity and a Griess assay was used to determine nitric oxide (NO) production. Concentration levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were determined by enzyme-linked immunosorbent assay. Protein expression levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), phosphorylated (p)-extracellular signal-regulated kinase (ERK) 1/2, p-c-Jun N-terminal kinases (JNK), p-p38 and inhibitor of κB-α were detected by western blot analysis. AP ethanol extract was revealed to inhibit xylene-induced ear edema in mice and carrageenan-induced paw edema in rats. Tiliroside significantly suppressed the overproduction of NO (P<0.01), but revealed no notable inhibition of the release of TNF-α and IL-6. In addition, tiliroside significantly downregulated the elevated expression levels of iNOS and COX-2 induced by LPS (P<0.01). The phosphorylation of JNK and p38 proteins were also significantly inhibited (P<0.01), however, tiliroside exhibited no obvious inhibition on the phosphorylation of ERK 1/2 and the degradation of IκB-α protein.In conclusion, the anti-inflammatory molecular mechanism of tiliroside may involve the downregulation of iNOS and COX-2 protein expression levels, and the inactivation of mitogen-activated protein kinase (MAPK)/JNK, in addition to the MAPK/p38 signaling pathway. IntroductionAgrimonia pilosa Ledeb (Rosaceae; AP) is a perennial herbaceous flowering plant distributed in the temperate regions of the Northern hemisphere, including Asia. As a traditional medicinal plant, it has been used for the treatment of abdominal pain, sore throat, headaches, bloody discharge, parasitic infections and eczema (1). Pharmacological research has revealed that AP extracts possess diverse pharmacological properties, including antinociceptive (1), antiplatelet (2) and antihemostatic effects (3), in addition to antioxidant (4), antiviral (5), antitumor (6), anti-inflammatory, anti-allergic (7,8), anti-microbial (9) and anti-hyperglycemic (10) activity, amongst others. Tiliroside, a glycosidic flavonoid (Fig. 1), is the major component of AP extracts. Tiliroside has been reported to exhibit the following: Anti-diabetic properties by enhancing glucose consumption by insulin-resistant HepG2 cells (11,12); antihypertensive and vasorelaxant effects in resistance arteries (13); anti-hyperglycemic, anti-hyperlipidemic and antioxidant activities (14)

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Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Tiliroside, the major component of Agrimonia pilosa Ledeb ethanol extract, inhibits MAPK/JNK/p38-mediated inflammation in lipopolysaccharide-activated RAW 264.7 macrophages

Jin

Song

Wang

et al. 2016

Experimental and Therapeutic Medicine

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“…The effect of AG extract on BV-2 cell viability was determined as described previously (Kim et al, 2012). Medium was discarded after treatment and DMSO was added to each well for the solubilization of formazan.…”

Section: Cell Viability Testmentioning

confidence: 99%

“…The effect of AG extract on nitrite concentration was determined as described previously (Kim et al, 2012). A range of dilutions of sodium nitrite were used to obtain standard curve with the amount of nitrite in each sample.…”

Section: Determination Of Nitric Oxide (No) Productionmentioning

confidence: 99%

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Acorus gramineus inhibits microglia mediated neuroinflammation and prevents neurotoxicity in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse model of Parkinson's disease

Jiang

Kim

et al. 2012

Journal of Ethnopharmacology

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Development of a liquid chromatography–mass spectrometry method for determination of agrimol B in rat plasma: application to preclinical pharmacokinetics

Wang

Liu

Liang

et al. 2014

Biomedical Chromatography

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A sensitive and accurate liquid chromatography coupled with mass spectrometry (LC-MS) method was developed for the determination of agrimol B, a main active ingredient isolated from Agrimonia pilosa Ledeb., in rat plasma. Chromatographic separation was achieved on a Zorbax CN column (150 × 4.6 mm, 5 µm), with isocratic elution consisting of acetonitrile and water (15:85, v/v) at a flow rate of 0.6 mL/min. Agrimol B and dryocrassin ABBA, an internal standard (IS), were analyzed by selected ion monitoring at m/z transitions of 681.3 and 819.4, respectively. This assay exhibited a good linearity with a correlation coefficient >0.99 and showed no endogenous interference with the analyte and IS. The limit of quantification of agrimol B was 8.025 ng/mL with acceptable precision and accuracy. The method was successfully applied in the pharmacokinetic study of agrimol B in rats after intravenous (1 mg/kg) and oral (2, 5 and 10 mg/kg) doses of agrimol B. The absolute bioavailability of agrimol B was 16.4-18.0% in rat. Our study clarifies the pharmacokinetic behavior of agrimol B in animals.

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Anti-inflammatory and anti-allergic effects of Agrimonia pilosa Ledeb extract on murine cell lines and OVA-induced airway inflammation

Cited by 51 publications

References 40 publications

Tiliroside, the major component of Agrimonia pilosa Ledeb ethanol extract, inhibits MAPK/JNK/p38-mediated inflammation in lipopolysaccharide-activated RAW 264.7 macrophages

Tiliroside, the major component of Agrimonia pilosa Ledeb ethanol extract, inhibits MAPK/JNK/p38-mediated inflammation in lipopolysaccharide-activated RAW 264.7 macrophages

Acorus gramineus inhibits microglia mediated neuroinflammation and prevents neurotoxicity in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse model of Parkinson's disease

Development of a liquid chromatography–mass spectrometry method for determination of agrimol B in rat plasma: application to preclinical pharmacokinetics

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