Anti-Inflammatory Activity of Unusual Lupane Saponins fromBupleurum fruticescens

Abstract

Extracts from Bupleurum fruticescens were examined for oral and topical anti-inflammatory activities. The BuOH extract proved to be active against carrageenan and tetradecanoylphorbol acetate acute edemas and allowed the isolation of three saponins identified by spectroscopic techniques as 3 beta-O-(O-alpha-L-rhamnopyranosyl-(1-->4)-O-[beta-D-glucopyranosyl- (1-->6)]-O-beta-D-glucopyranosyl)lup-20(29)-ene-23,28-dioic acid (fruticesaponin A), 3 beta-O-(O-alpha-L-rhamnopyranosyl-(1-->4)-O-beta-D-glucopyranosyl) lup-20(29)-ene-23,28-dioic acid 28-O-beta-D-glucopyranosyl ester (fruticesaponin B), and 3 beta-O-(O-alpha-L-rhamnopyranosyl-(1-->4)-O-[beta-D-glucopyranosyl- (1-->6)]-O-beta-D-glucopyranosyl)-lup-20(29)-ene-23,28-dioic acid 28-O-beta-D-glucopyranosyl ester (fruticesaponin C). These compounds were studied against carrageenan, tetradecanoylphorbol acetate, arachidonic acid and ethyl phenylpropiolate acute edemas. Fruticesaponin B, a bidesmosidic saponin with an unbranched saccharide moiety was the most active in all the tests applied.

Phytochemical and HPTLC Studies on Fruit Extracts of Momordica cymbalaria Fenzl, a Medicinally Important Plant

“…These compounds are biologically active and exhibit diversified activities. Just et al (1998) reported the inhibitory effect of saponins on inflamed cells and this has supported the usefulness of this plant in managing inflammation. Saponins cause the leakage of proteins and degradation of cell wall enzymes (Zablotowicz et al, 1996).…”

In vivoanti-inflammatory properties of aerial parts ofNasturtium officinale

“…In the first phase (0-1 h after injection of carrageenan) histamine, bradykinin, and serotonin are involved (Crunkhorn & Meacock, 1971;Vinegar et al, 1969), while the second phase (after 1 h) is attributed to infiltration of leucocytes, production of oxygen-derived free radicals and release of prostaglandins, protease, and lysosome (Di et al, 1971;Halici et al, 2007;Vircheva et al, 2011). The ethanol extract of N. officinale exhibited prominent protective effects on the development of paw swelling by carrageenan over a period of 4 h. This finding indicates the inhibitory effects of N. officinale against release of the pro-inflammatory mediators involved in the two phases of the carageenan test (Just et al, 1998). Our pathological examination confirmed that the watercress extract reduced edema the migration of leucocytes into carrageenan-treated paws.…”

Phytochemical Screening of Medicinal Plants of the Kakamega Country, Kenya Commonly Used against Cancer

“…Lignans strongly suppressed the incorporation of [ Saponins are steroid or triterpenoid glycosides which derive their name from their ability to form stable, soap-like foams in aqueous solutions and several biological effects which include immunostimulant and antitumourigenic properties [17]. Saponins have also been shown to be anti-inflammatory [18]. In the present study, TLC and staining with anisyl aldehyde in sulfuric acid was used to ascertain its presence.…”