Anti-inflammatory activity of lupine (Lupinus angustifolius L.) protein hydrolysates in THP-1-derived macrophages

Abstract

The effect of two different lupine protein hydrolysates (LPHs) on in vitro macrophage activation in a THP-1-derived macrophage model was investigated. THP-1-derived macrophages were exposed to RPMI medium containing two LPHs obtained by enzymatic hydrolysis using two different proteases: Izyme AL and Alcalase 2.4 L. Cytokine's expression was measured by quantitative PCR. THP-1-derived macrophages exhibited attenuated expression of proinflammatory cytokines (tumour necrosis factor (TNF), IL-6, IL-1β) and increased expression of anti-inflammatory marker genes (chemokine (C-C motif) ligand 18 (CCL18)) relative to control without LPH. The anti-inflammatory effect of both hydrolysates favoured M2 polarization by quenching C-C chemokine receptor type 2 (CCR2) expression and migratory capacity. Furthermore, LPHs significantly decreased nitric oxide production. Moreover, LPHs promoted the survival of human THP-1-derived macrophages. Therefore, inclusion of LPHs in foods may help to prevent chronic diseases associated with chronic inflammation. 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 2 ANTI-INFLAMMATORY ACTIVITY OF LUPINE (Lupinus angustifolius L.) PROTEIN HYDROLYSATES IN THP-1-DERIVED MACROPHAGES AbstractThe effect of two different lupine protein hydrolysates (LPHs) on in vitro macrophage activation in a THP-1-derived macrophage model was investigated. THP-1-derived macrophages were exposed to RPMI medium containing two LPHs obtained by enzymatic hydrolysis using two different proteases: Izyme AL and Alcalase 2.4 L.Cytokine's expression was measured by quantitative PCR. THP-1-derived macrophages exhibited attenuated expression of proinflammatory cytokines (tumour necrosis factor (TNF), IL-6, IL-1β) and increased expression of anti-inflammatory marker genes (chemokine (C-C motif) ligand 18 (CCL18)) relative to control without LPH. The antiinflammatory effect of both hydrolysates favoured M2 polarization by quenching C-C chemokine receptor type 2 (CCR2) expression and migratory capacity. Furthermore, LPHs significantly decreased nitric oxide production. Moreover, LPHs promoted the survival of human THP-1-derived macrophages. Therefore, inclusion of LPHs in foods may help to prevent chronic diseases associated with chronic inflammation.

GPETAFLR: A new anti-inflammatory peptide from Lupinus angustifolius L. protein hydrolysate

“…These results were subsequently confirmed by the reduction of cytokine expression in THP-1-derived macrophages, and this was the first report to describe the anti-inflammatory effect in these cells and influence the control of macrophage polarisation in the context of an inflammatory state (Millán-Linares et al, 2014a).…”

Phytochemical composition and bioactivities of lupin: a review

“…Recently, in vitro anti‐inflammatory properties of protein hydrolysates from seeds of L. angustifolius have been investigated using a THP‐1‐derived macrophage model and inhibition of enzymes involving in the inflammatory pathway (Millán‐Linares et al ., ). Lupin protein hydrolysate (LPH) was attained by hydrolysis of lupin protein isolate (LPI) using two proteases, izyme and alcalase.…”

Ovalbumin Hydrolysates Inhibit Nitric Oxide Production in LPS-induced RAW 264.7 Macrophages

“…Many peptides produced from various protein sources, such as soy (de Mejia and Dia, 2009), fish and lupine (Millán-Linares et al, 2014), are known to have anti-inflammatory properties. These peptides inhibit the production of pro-inflammatory cytokines and NO in immune cells after stimulation.…”