Ovalbumin Hydrolysates Inhibit Nitric Oxide Production in LPS-induced
                    RAW 264.7 Macrophages

Kim, Hyun Suk; Lee, Jae Hoon; Moon, Sun Hee; Ahn, Dong U.; Paik, Hyun‐Dong

doi:10.5851/kosfa.2020.e12

Cited by 22 publications

(12 citation statements)

References 36 publications

(41 reference statements)

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“…In our present study, OVT showed immunoregulatory activity, with NO production of LPS‐induced RAW264.7 treated with low concentration of OVT being inhibited, and increased secretion of the NO in a dose‐dependent manner in OVT‐induced RAW264.7 cells. These observations are in accordance with the previous finding that food‐derived protein anti‐inflammatory extracts markedly inhibit the release of NO in LPS‐stimulated RAW264.7 macrophages (Kim et al., 2020 ; Tagashira et al., 2018 ). Kim et al.…”

Section: Discussionsupporting

confidence: 93%

“…These observations are in accordance with the previous finding that food-derived protein anti-inflammatory extracts markedly inhibit the release of NO in LPS-stimulated RAW264.7 macrophages (Kim et al, 2020;Tagashira et al, 2018). Kim et al (2020) found that the production of NO is inhibited by ovalbumin hydrolysate through reducing the phosphorylation of JNK and ERK in LPS-stimulated RAW264.7. It is speculated that the possible reason is that OVT can control the NO release process by MAPK signaling pathway.…”

Section: Discussionsupporting

confidence: 93%

“…Kim et al. ( 2020 ) found that the production of NO is inhibited by ovalbumin hydrolysate through reducing the phosphorylation of JNK and ERK in LPS‐stimulated RAW264.7. It is speculated that the possible reason is that OVT can control the NO release process by MAPK signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

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Ovotransferrin exerts bidirectional immunomodulatory activities via TLR4‐mediated signal transduction pathways in RAW264.7 cells

Zhu

et al. 2021

Food Science & Nutrition

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 93%

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Ovotransferrin exerts bidirectional immunomodulatory activities via TLR4‐mediated signal transduction pathways in RAW264.7 cells

Zhu

et al. 2021

Food Science & Nutrition

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“…5 ). In addition to the activation of NF-κB, LPS also activates a series of major intracellular signaling transduction pathways, such as ERK, which serves crucial roles in inflammation ( 16 , 17 ). Therefore, the expression levels and phosphorylation status of ERK1/2 in EpCs and MPs were analyzed.…”

Section: Resultsmentioning

confidence: 99%

Mechanisms of the lipopolysaccharide‑induced inflammatory response in alveolar epithelial cell/macrophage co‑culture

Qin

Chen

et al. 2020

Exp Ther Med

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The interaction between alveolar epithelial cells (EpCs) and macrophages (MPs) serves an important role in initiating and maintaining inflammation in chronic pulmonary diseases. The aim of the present study was to investigate the molecular mechanisms of the inflammatory response in co-cultured EpCs and MPs. Briefly, a co-culture system of A549 (EpCs) and THP-1 (monocyte/MPs) cells was established in a filter-separated Transwell plate to evaluate the inflammatory response.

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“…In particular, various proteins present in egg white and yolk (ovalbumin, ovotransferrin, ovomucin, IgY, phosvitin, etc.) have been reported to have various functional activities such as antioxidant, anti-inflammatory, immune-enhancing, and anti-biofilm activities ( Abeyrathne et al, 2016 ; Kim et al, 2020b ; Kim et al, 2020c ; Lee et al, 2018 ). Egg yolk protein is produced as a by-product after extracting egg yolk lecithin with an organic solvent.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Immune-Modulating Activities of Egg Yolk Protein Extracts

Lee¹,

Lee²,

Paik³

et al. 2022

Food Sci Anim Resour

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Egg yolk is widely used to extract lecithin, which is utilized in the food and cosmetics industry. After lecithin is removed, the rest of egg yolk is generated as a by-product. Thus, it is necessary to properly utilize it. In this study, egg yolk protein extracts were produced using ethanol (EYE-E) and water (EYE-W). Their antioxidant and immunomodulatory effects were then evaluated. Antioxidant activities of EYE-E and EYE-W were determined using cellular antioxidant capacity (CAC) assay and comet assay. EYE-E and EYE-W showed significant (p<0.05) scavenging effects on intracellular reactive oxygen species (ROS) in a dose dependent manner. At a concentration of 50 μg/mL, EYE-W showed higher (p<0.05) antioxidant activity than EYE-E. EYE-E and EYE-W also exhibited protective effects against DNA damage caused by oxidative stress. After treatment with EYE-E and EYE-W, DNA damage level of 48.7% due to oxidative stress was decreased to 36.2% and 31.8% levels, respectively. In addition, EYE-E and EYE-W showed immunomodulatory effects by regulating Th1 cytokines (TNF-α and IL-2) and Th2 cytokines (IL-10 and IL-4) in Balb/c mouse splenocytes. These data suggest that EYE-E and EYE-W could be used as functional food ingredients with excellent antioxidant and immunomodulatory activities in the food industry.

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Ovalbumin Hydrolysates Inhibit Nitric Oxide Production in LPS-induced RAW 264.7 Macrophages

Cited by 22 publications

References 36 publications

Ovotransferrin exerts bidirectional immunomodulatory activities via TLR4‐mediated signal transduction pathways in RAW264.7 cells

Ovotransferrin exerts bidirectional immunomodulatory activities via TLR4‐mediated signal transduction pathways in RAW264.7 cells

Mechanisms of the lipopolysaccharide‑induced inflammatory response in alveolar epithelial cell/macrophage co‑culture

Antioxidant and Immune-Modulating Activities of Egg Yolk Protein Extracts

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