Anti-inflammatory activity of gold and graphene oxide nanoparticles in-vitro study

Kadhim, Rua J.; Karsh, Esraa H.; Jabir, Majid S.

doi:10.1063/5.0000169

Cited by 9 publications

(6 citation statements)

References 16 publications

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“…ab1977742; Abcam, Cambridge, MA, USA) was estimated using an enzyme-linked immunosorbent assay (ELISA) kit, according to the manufacturer's guidance. The absorbance was read using an ELISA plate reader at 570 nm [42].…”

Section: Enzyme-linked Immunosorbent Assaymentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles Using Annona muricata Extract as an Inducer of Apoptosis in Cancer Cells and Inhibitor for NLRP3 Inflammasome via Enhanced Autophagy

et al. 2021

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Annona muricata is one of the most important traditional medicinal plants which contains numerous chemicals that exhibit various pharmacological properties. In this study, silver nanoparticles were prepared using A. muricata peel extract as a reducing agent and the effect was enhanced through A. muricata like pharmaceutical activity. AgNPs formation was confirmed by color changes, UV-visible spectroscopy, SEM, DLS, and XRD. The anti-proliferative activity of AgNPs against THP-1, AMJ-13, and HBL cell lines was studied. Apoptotic markers were tested using AO/EtBr staining assay, cell cycle phases using flowcytometry, and the expression of P53. Autophagy takes an essential part in controlling inflammasome activation by primary bone marrow-derived macrophages (BMDMs). We report novel functions for AgNPs-affected autophagy, represented by the control of the release of IL-1β, caspase-1, adaptor protein apoptosis-associated speck-like protein containing a CARD (ASC), and NLRP3 in BMDMs following treatment with LPS+ATP. The current study revealed that the AgNPs inhibited THP-1 and AMJ-13 cell proliferation. Meanwhile, the AgNPs significantly increased autophagy and reduced IL-1b and NLRP3 levels in both in vivo and in vitro models. The secretion of IL-1β was reduced whereas the degradation of NLRP3 inflammasome was enhanced. These findings propose that AgNPs apply an anti-proliferative activity against THP-1 and AMJ-13 cells through the stimulation of apoptosis via mitochondrial damage and induction of p53 protein pathway. In addition, AgNP-induced autophagy reduced the levels of IL-1β and NLRP3 inflammasome activation. This indicated that the AgNPs augment autophagy controlled by the IL-1β pathway via two different novel mechanisms. The first one is regulating activation of the IL-1 β, caspae-1, and ASC, while the second is NLRP3 targeting for lysosomal degradation. Overall, this study suggests that AgNPs could be a potent therapy for various types of cancer and an alternative treatment for preventing inflammation via enhancing autophagy.

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Section: Enzyme-linked Immunosorbent Assaymentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles Using Annona muricata Extract as an Inducer of Apoptosis in Cancer Cells and Inhibitor for NLRP3 Inflammasome via Enhanced Autophagy

et al. 2021

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“…For preparation, procurement, and characterization of nanoparticle materials known methodology and spectro-analytical and morphology observation technique, UV-visible and scanning electron microscope (SEM) were used to measure and study the GNPs and GOFs [ 36 ].…”

Section: Methodsmentioning

confidence: 99%

Gold Nanoparticles and Graphene Oxide Flakes Enhance Cancer Cells’ Phagocytosis through Granzyme-Perforin-Dependent Biomechanism

et al. 2021

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The study aimed to investigate the roles of gold nanoparticles (GNPs) and graphene oxide flakes (GOFs) as phagocytosis enhancers against cancer cells. The nanomaterials were characterized through SEM and UV-VIS absorptions. The GNPs and GOFs increased the macrophages’ phagocytosis ability in engulfing, thereby annihilating the cancer cells in both in vitro and in vivo conditions. The GNPs and GOFs augmented serine protease class apoptotic protein, granzyme, passing through the aquaporin class protein, perforin, with mediated delivery through the cell membrane site for the programmed, calibrated, and conditioned cancer cells killing. Additionally, protease inhibitor 3,4-dichloroisocoumarin (DCI) significantly reduced granzyme and perforin activities of macrophages. The results demonstrated that the GOFs and GNPs increased the activation of phagocytic cells as a promising strategy for controlling cancer cells by augmenting the cell mortality through the granzyme-perforin-dependent mechanism.

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“…The nanotechnology provides extensive and effective nanomaterials and tools that could be used in the detection and treatment of different inflammatory disorders and conditions. Some in vitro andin vivo experiments have been performed to examine the impact of nanoparticles on the development of inflammation and its subsequent disorders (Kadhim, Karsh, & Jabir, 2020). By modulating the nanoparticles, they can be directed to pass from the metabolic barriers and deliver drugs to particular targets without interacting with other cells.…”

Section: Figurementioning

confidence: 99%

Potential of nanoparticles and nanopolymers in treatment of age-associated diseases

Saadatpour

Mohammadipanah

Zohourian

et al. 2021

Preprint

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Aging is an inevitable process caused by the accumulation of degenerative destructions, which ultimately leads to organism death. As the aging process occurs at the molecular, cellular, and tissue levels, understanding the whole details is the prerequisite for the development of anti-aging therapy. More than 300 compounds of different sources have been reported with the anti-aging activity that slow aging and extend lifespan through regulating single or multiple signaling pathways. Recent innovations in nanotechniques could lead to the development of nanomaterials having anti-aging effects or acting as nanocarrier systems and distributers of anti-aging drugs. In this review, we summarized the molecular mechanisms of longevity and the prospect of developing anti-aging nanomaterials targeting aging pathways.

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Anti-inflammatory activity of gold and graphene oxide nanoparticles in-vitro study

Cited by 9 publications

References 16 publications

Green Synthesis of Silver Nanoparticles Using Annona muricata Extract as an Inducer of Apoptosis in Cancer Cells and Inhibitor for NLRP3 Inflammasome via Enhanced Autophagy

Green Synthesis of Silver Nanoparticles Using Annona muricata Extract as an Inducer of Apoptosis in Cancer Cells and Inhibitor for NLRP3 Inflammasome via Enhanced Autophagy

Gold Nanoparticles and Graphene Oxide Flakes Enhance Cancer Cells’ Phagocytosis through Granzyme-Perforin-Dependent Biomechanism

Potential of nanoparticles and nanopolymers in treatment of age-associated diseases

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