Gold Nanoparticles and Graphene Oxide Flakes Enhance Cancer Cells’ Phagocytosis through Granzyme-Perforin-Dependent Biomechanism

Al-Omar, Mohsen S.; Jabir, Majid S.; Karsh, Esraa H.; Kadhim, Rua J.; Sulaiman, Ghassan M.; Taqi, Zainab J.; Khashan, Khawla S.; Mohammed, Hamdoon A.; Khan, Riaz A.; Mohammed, Salman A. A.

doi:10.3390/nano11061382

Cited by 24 publications

(6 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the surface modification of CNPs via decorating with metal (Ag, Au, and Pt) and metal oxide nanoparticles (ZnO, Fe 2 O 3 , and TiO 2 ) is the most functional methods to enhance the antibacterial performance of and reduce the adverse effects of the metal and metal oxide materials. Recently, the development of carbon nanostructures decorated with different types of NPs demonstrates a promising process for different applications [7][8][9][10]. Among them, copper materials demonstrated the ability to modify cancer cell metabolism and induce cell death.…”

Section: Introductionmentioning

confidence: 99%

Copper Oxide Nanoparticle-Decorated Carbon Nanoparticle Composite Colloidal Preparation through Laser Ablation for Antimicrobial and Antiproliferative Actions against Breast Cancer Cell Line, MCF-7

Mohammed

Khashan

Jabir

et al. 2022

BioMed Research International

Self Cite

View full text Add to dashboard Cite

Copper oxide (CuO) nanoparticle- (NP-) decorated carbon NPs (CNPs) were produced as colloidal suspension through pulsed laser ablation technique in liquid (PLAL) medium. The antimicrobial activity of the produced NPs was tested against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), and anticancer activity was tested against breast cancer cell line, MCF-7, together with the biocompatibility assessment of these NPs. The X-ray diffraction (XRD) patterns of the obtained CNPs showed peaks at 26.58° and 43.78° (2θ) identical to (002) and (111) planes, respectively, of the carbon phases. It also displayed new peaks at 38.5° and 48.64° (2θ) after doping with CuO NPs. Transmission electron microscope (TEM) images revealed the crystalline nature with the spherical shape of the prepared CNPs with 5-40 nm diameter ranges. In addition, the NP effects on the bacterial cell walls and nucleic acid were confirmed using a scanning electron microscope (SEM) and microscopic fluorescence analysis. The NPs showed antibacterial activity through SEM examinations against the pathogenic microbial species, S. aureus and E. coli. In the cellular material release assay, the optical density of the bacterial cells, treated with NPs, displayed a significant increase with the time of exposure to NPs, and the cytotoxicity reached more than 80% of the level for the CNPs decorated with CuO NPs. The morphology of the MCF-7 cells treated with NPs decreased numbers, and the loss of contact with the surrounding cells was observed. These results confirmed that the CNPs decorated with CuO NPs have no observable side effects and can be safely used for therapeutic applications. It is also noteworthy that it is the first report of preparation of CuO NPs decorated with CNPs (CuO NPs-CNPs) by PLAL, and the produced NPs showed antimicrobial antiproliferative activities against breast cancer cell lines, MCF-7. The main advantage of the PLAL technique of synthesizing CuO NPs-CNPs provided a two-step, cost-effective, and eco-friendly method.

show abstract

Section: Introductionmentioning

confidence: 99%

Copper Oxide Nanoparticle-Decorated Carbon Nanoparticle Composite Colloidal Preparation through Laser Ablation for Antimicrobial and Antiproliferative Actions against Breast Cancer Cell Line, MCF-7

Mohammed

Khashan

Jabir

et al. 2022

BioMed Research International

Self Cite

View full text Add to dashboard Cite

show abstract

“…The absence of antimicrobial activity for the CNF substrate was because the Cu2O-NPs were encapsulated inside the CNF matrix and not in contact with the pathogen cells. Although the nanostructure of carbon nanotube materials provided antimicrobial properties [17,[36][37], this CNF sample not exhibiting the zone of inhibition may be due to the larger diameter size of carbon fiber. Samadian et al [5] reported that the degree of genotoxicity for electro-spun CNFs derived from PAN followed by heat treatment may be less than that of traditional CNFs.…”

Section: Antimicrobial Activitymentioning

confidence: 89%

Synthesis and antimicrobial studies of nano-copper doped carbon substrates; activated carbon, reduced graphene oxide, and carbon nanofiber

CHANTHEE¹,

JIRASANGTHONG²,

ASASVATESANUPAP³

et al. 2022

J Met Mater Miner

View full text Add to dashboard Cite

Copper oxides (CuxO) have received considerable attention as a result of their biological activity. Nanoparticles (NPs) of CuxO attached to different substrates exhibit a wide spectrum of antimicrobial activity against bacteria and viruses, with similar properties to silver. The antimicrobial activity of CuxO-NPs doped on distinctive carbon materials was investigated for three carbon substrates: apricot stone activated carbon (AAC), reduced graphene oxide (rGO) and carbon nanofiber (CNF). The CuxO-NPs (5 wt%) doped AAC and rGO substrates were prepared by impregnation of copper nitrate followed by a thermal treatment process, while a similar weight of CuxO-NPs doped CNF was fabricated by electrospinning copper nitrate with polyacrylonitrile precursor, followed by carbonization. The CuxO species and chemical functions were characterized by X-ray diffraction and Fourier transform infrared spectroscopy, respectively. Surface morphology was measured using scanning electron microscopy. The antimicrobial activities of the substrates were evaluated by inhibition zone measurement of Staphylococcus aureus and Escherichia coli. The results demonstrated significant inhibition distances for different carbon substrates. Interestingly, CuxO-NPs doped over both AAC and rGO surfaces revealed clear zones against bacteria, whereas the inhibition zone was not recorded for CuxO-NPs doped over a CNF substrate. Various parameters such as carbon substrates, particle size, and copper oxide species were investigated.

show abstract

“…In recent years, among all noble metal nanoparticles, gold nanoparticles have received extensive attention owing to their biocompatibility, tunable surface plasmon resonance (SPR) and unique optoelectronic characteristics (Boisselier & Astruc, 2009 ; Zeiri et al., 2014 ). It has been reported that gold nanoparticles together with the micro-environmental stimuli could help activate the immune system (MacParland et al., 2017 ; Al-Omar et al., 2021 ). Furthermore, gold nanoparticles possess a rough surface with a high surface-to-volume ratio, enabling these particles with high cohesive ability to form various biomolecules, providing us an opportunity to biosynthesize nanocomposites with less toxicity (Ahn et al., 2018 ; Khan et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Green synthesized gold nanoparticles from Pseudobulbus Cremastrae seu Pleiones show efficacy against hepatic carcinoma potentially through immunoregulation

Zhu

Liu

et al. 2022

Drug Delivery

View full text Add to dashboard Cite

Nanobiotechnology, the interface between biology and nanotechnology, has recently emerged in full bloom in the medical field due to its minimal side-effects and high efficiency. To broaden the application of nanobiotechnology, we composed gold nanoparticles from the extract of Pseudobulbus Cremastrae seu Pleiones (PCSP) using an efficient and green procedure. The biosynthesized Au nanoparticles containing PCSP (PCSP-AuNPs) were characterized by UV-vis spectroscopic, transmission electron microscopy (TEM), atomic force microscopy (AFM), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FT-IR), and Energy Dispersive X-ray (EDAX). After verifying the stability of PCSP-AuNPs, we detected its biosafety and immune-modulatory effects on RAW264.7 in vitro using NO assay, ELISA (TNF-α, IL-12p70, and IL-1β), and CCK-8 test. Furthermore, we examined the direct in vitro effects of PCSP-AuNPs on hepatocellular carcinomas (HCCs). Finally, we evaluated the immune regulation of PCSP-AuNPs using a mouse model with H22-tumor by testing the index of immune organs, splenic lymphocyte proliferation, cytokines levels (TNF-α and IL-10), and the CD4+/CD8+ cell ratio in the peripheral blood. Immunohistochemical analyses including H&E and PCNA staining were performed to investigate the anti-cancer efficacy and biocompatibility of PCSP-AuNPs. We found that PCSP-AuNPs not just possessed low toxicity, but also improved the immune-mediated antitumor response as compared to PCSP alone, suggesting its potential as a novel and efficient drug for liver cancer therapy.

show abstract

Gold Nanoparticles and Graphene Oxide Flakes Enhance Cancer Cells’ Phagocytosis through Granzyme-Perforin-Dependent Biomechanism

Cited by 24 publications

References 44 publications

Copper Oxide Nanoparticle-Decorated Carbon Nanoparticle Composite Colloidal Preparation through Laser Ablation for Antimicrobial and Antiproliferative Actions against Breast Cancer Cell Line, MCF-7

Copper Oxide Nanoparticle-Decorated Carbon Nanoparticle Composite Colloidal Preparation through Laser Ablation for Antimicrobial and Antiproliferative Actions against Breast Cancer Cell Line, MCF-7

Synthesis and antimicrobial studies of nano-copper doped carbon substrates; activated carbon, reduced graphene oxide, and carbon nanofiber

Green synthesized gold nanoparticles from Pseudobulbus Cremastrae seu Pleiones show efficacy against hepatic carcinoma potentially through immunoregulation

Contact Info

Product

Resources

About