Anti-inflammatory activity of c-phycocyanin in lipopolysaccharide-stimulated RAW 264.7 macrophages

Cherng, Shiou-Chi; Cheng, Shin‐Nan; Tarn, Andrew; Chou, Tz-Chong

doi:10.1016/j.lfs.2007.09.009

Cited by 117 publications

(73 citation statements)

References 31 publications

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“…Recent reports showed that PC could significantly inhibit the LPS-induced nitrite production and iNOS protein expression accompanied by an attenuation of TNF-␣ formation (Cherng et al, 2007). Pre-or post-treatment with PC significantly attenuated carrageenan-induced inflammatory nociception and the induction of iNOS and COX-2 accompanied by an inhibition of the formation of TNF-␣, E 2 and myeloperoxidase activity (Shih et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…PC has been shown to possess significant antioxidant, radical-scavenging, anti-inflammatory, hepatoprotective, radical scavenging, antiarthritic, neuroprotective and anti-tumor effects (Romay et al, 2003;Li et al, 2005;Cherng et al, 2007;Sathyasaikumar et al, 2007). Therefore, in the present study an attempt was made to investigate whether PC has the protective effect on PQ-induced acute lung injury in a rodent model of rats.…”

Section: Introductionmentioning

confidence: 96%

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The protective effect of C-phycocyanin on paraquat-induced acute lung injury in rats

Sun

Zhang²,

Yan³

et al. 2011

Environmental Toxicology and Pharmacology

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

The protective effect of C-phycocyanin on paraquat-induced acute lung injury in rats

Sun

Zhang²,

Yan³

et al. 2011

Environmental Toxicology and Pharmacology

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“…33) Overproduction of NO derived from iNOS plays an important role in the pathogenesis of inflammation. 34) On the other hand, PGE 2 is a pleiotropic mediator that causes pain, swelling, and stiffness. 35) Hence, inhibitors of NO and PGE 2 production could be used as potential antiinflammatory agents.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory Effects of Violaxanthin Isolated from Microalga <i>Chlorella ellipsoidea</i> in RAW 264.7 Macrophages

Soontornchaiboon

Joo

Kim

2012

Biological & Pharmaceutical Bulletin

124

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Violaxanthin is a major carotenoid of microalgae Chlorella ellipsoidea and is also found in dark-green leafy vegetables, such as spinach. In this study, the anti-inflammatory effect of violaxanthin isolated from C. ellipsoidea was examined using lipopolysaccharide (LPS)-stimulated RAW 264.7 mouse macrophage cells. In addition, the anti-inflammatory activity and mechanism of action of purified violaxanthin was assessed using various assays, such as quantitative real-time polymerase chain reaction (PCR), Western blotting, and electrophoretic-mobility shift assay (EMSA). The results of this combined analysis revealed that violaxanthin significantly inhibited nitric oxide (NO) and the prostaglandin E 2 (PGE 2 ). Interestingly, violaxanthin effectively inhibited LPS-mediated nuclear factor-κB (NF-κB) p65 subunit translocation into the nucleus, suggesting that the violaxanthin anti-inflammatory activity may be based on inhibition of the NF-κB pathways. In conclusion, violaxanthin of C. ellipsoidea holds promise for use as a potential anti-inflammatory agent for either therapeutic or functional adjuvant purposes.

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“…Moreover, it plays an important role in many biochemical techniques due to its fluorescent properties [15]. Recently, it has also been observed that phycocyanin shows anti-inflammatory and anti-cancer properties [10,20].…”

Section: Introductionmentioning

confidence: 99%

Separation of phycocyanin from Spirulina platensis using ion exchange chromatography

Silveira

Quines

Burkert

et al. 2008

Bioprocess Biosyst Eng

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This paper presents the evaluation of some important parameters for the purification of phycocyanin using ion exchange chromatography. The influences of pH and temperature on the equilibrium partition coefficient were investigated to establish the best conditions for phycocyanin adsorption. The equilibrium isotherm for the phycocyanin-resin system was also determined. The separation of phycocyanin using the Q-Sepharose ion exchange resin was evaluated in terms of the pH and elution volume that improved the increase in purity and recovery. The highest partition coefficients were obtained in the pH range from 7.5 to 8.0 at 25 degrees C. Under these conditions the equilibrium isotherm for phycocyanin adsorption was well described by the Langmuir model, attaining a Q (m) of 22.7 mg/mL and K (d) of 3.1 x 10(-2) mg/mL. The best conditions for phycocyanin purification using the ion exchange column were at pH 7.5 with an elution volume of 36 mL, obtaining 77.3% recovery and a 3.4-fold increase in purity.

show abstract

Anti-inflammatory activity of c-phycocyanin in lipopolysaccharide-stimulated RAW 264.7 macrophages

Cited by 117 publications

References 31 publications

The protective effect of C-phycocyanin on paraquat-induced acute lung injury in rats

The protective effect of C-phycocyanin on paraquat-induced acute lung injury in rats

Anti-inflammatory Effects of Violaxanthin Isolated from Microalga <i>Chlorella ellipsoidea</i> in RAW 264.7 Macrophages

Separation of phycocyanin from Spirulina platensis using ion exchange chromatography

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