Anti-inflammatory activities of cucurbitacin E isolated from Citrullus lanatus var. citroides: Role of reactive nitrogen species and cyclooxygenase enzyme inhibition

Abdelwahab, Siddig Ibrahim; Hassan, Loiy Elsir Ahmed; Sirat, Hasnah Mohd; Yagi, Sakina; Koko, Waleed S.; Mohan, Syam; Taha, Manal Mohamed Elhassan; Ahmad, Sadeem; Chuen, Cheah Shiau; Narrima, Putri; Rais, Mohd Mustafa; Hadi, A. Hamid A.

doi:10.1016/j.fitote.2011.08.002

Cited by 95 publications

(61 citation statements)

References 24 publications

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“…However, analysis of NF-kB after Ac2-26 treatment showed that the anti-inflammatory effects of AnxA1 are independent of NF-kB translocation and are consistent with results observed in RAW264-7 macrophages activated by LPS and treated with AnxA1 (47). Another study using the same macrophage cell line revealed reduced activity of the COX-2 pathway independent of NF-kB after phytotherapy (48).…”

Section: Effects Of Anxa1 On Il-6 Il-8 and Cox-2 Gene Expression Insupporting

confidence: 82%

Anti-Inflammatory Mechanisms of the Annexin A1 Protein and Its Mimetic Peptide Ac2-26 in Models of Ocular Inflammation In Vivo and In Vitro

Girol

Mimura

Drewes

et al. 2013

The Journal of Immunology

103

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Annexin A1 (AnxA1) is a protein that displays potent anti-inflammatory properties, but its expression in eye tissue and its role in ocular inflammatory diseases have not been well studied. We investigated the mechanism of action and potential uses of AnxA1 and its mimetic peptide (Ac2-26) in the endotoxin-induced uveitis (EIU) rodent model and in human ARPE-19 cells activated by LPS. In rats, analysis of untreated EIU after 24 and 48 h or EIU treated with topical applications or with a single s.c. injection of Ac2-26 revealed the anti-inflammatory actions of Ac2-26 on leukocyte infiltration and on the release of inflammatory mediators; the systemic administration of Boc2, a formylated peptide receptor (fpr) antagonist, abrogated the peptide’s protective effects. Moreover, AnxA1−/− mice exhibited exacerbated EIU compared with wild-type animals. Immunohistochemical studies of ocular tissue showed a specific AnxA1 posttranslational modification in EIU and indicated that the fpr2 receptor mediated the anti-inflammatory actions of AnxA1. In vitro studies confirmed the roles of AnxA1 and fpr2 and the protective effects of Ac2-26 on the release of chemical mediators in ARPE-19 cells. Molecular analysis of NF-κB translocation and IL-6, IL-8, and cyclooxygenase-2 gene expression indicated that the protective effects of AnxA1 occur independently of the NF-κB signaling pathway and possibly in a posttranscriptional manner. Together, our data highlight the role of AnxA1 in ocular inflammation, especially uveitis, and suggest the use of AnxA1 or its mimetic peptide Ac2-26 as a therapeutic approach.

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Section: Effects Of Anxa1 On Il-6 Il-8 and Cox-2 Gene Expression Insupporting

confidence: 82%

Anti-Inflammatory Mechanisms of the Annexin A1 Protein and Its Mimetic Peptide Ac2-26 in Models of Ocular Inflammation In Vivo and In Vitro

Girol

Mimura

Drewes

et al. 2013

The Journal of Immunology

103

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“…To the best of our knowledge, researchers have focused on the antioxidant and phytochemical components of C. lanatus fruit seeds and peels [19] with scanty information on the juice extract of mesocarp rind. The fruit of C. lanatus is consumed universally with the mesocarp rind always being discarded.…”

Section: Discussionmentioning

confidence: 99%

“…Morphologically, the leaves are simple, alternate on long petioles, cordate with seven shallow lobes and variously serrated margins, very hairy on the abaxial surface, acute, deep green, and about 7-15 cm in diameter [16]. The antioxidant activities, anti-inflammatory effects and several essential nutrients of C. lanatus fruits have been reported [18,19]. The juice or pulp of C. lanatus is consumed by people, while the rind and the seeds are usually discarded as agro-wastes [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Protective effect of juice extract of mesocarp rind of Citrullus lanatus in carbon tetrachloride-induced oxidative stress in rats

Bamisaye¹,

Sulyman²,

Ajani³

et al. 2017

JCLM

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“…Terpenoids, the characteristic feature of the family, Cucurbitaceae [17,18] are attributed to various pharmacological claims including antiinflammatory, anticancer, antimalarial, antiviral and antibacterial activities [19] . Literature data abounds in examples that terpenic compounds like cucurbitacin IIb and cucurbitacin E isolated from certain Cucurbitaceae members are promising candidates exhibiting antiinflammatory effects [20][21][22] . To the best of our knowledge, literature pertaining to the antiinflammatory property of any terpenic compound isolated from S. amplexicaulis is still in lacunae.…”

mentioning

confidence: 99%

Evaluation of Anti-inflammatory and Antioxidant Properties of Crude Extract and Forskolin from Solena amplexicaulis Leaf

Krishnamoorthy¹,

Senguttuvan²

2016

pharmaceutical-sciences

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Karthika, et al.: In vivo Biological Activities of S. amplexicaulisLeaves of Solena amplexicaulis are traditionally used as a remedy for the treatment of inflammation related disorders in the form of decoction by the Paliyar tribal community of Theni district, Tamil Nadu, India. This study was designed to investigate the antiinflammatory and antioxidant potential of crude methanol leaf extract and the isolated compound, forskolin from S. amplexicaulis. Their effects were evaluated by carrageenan-induced paw edema in experimental rats for 4 h. In vivo antioxidant and histopathological studies were also performed. Therapeutic potential of forskolin was further substantiated using PASS software. The oral administration of forskolin (10 mg/kg) exerted potent antiinflammatory activity by reducing paw edema (87.79%) than the crude extract (150 and 300 mg/kg) and it was comparable with the standard drug, indomethacin (10 mg/kg, 93.89%). Consistent with these findings, antioxidant, histopathological architecture and PASS software also reflected its prominent curative property. The obtained results therefore suggest that the compound, forskolin obtained from S. amplexicaulis is considered to be a valuable source of remedy for inflammation.

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Anti-inflammatory activities of cucurbitacin E isolated from Citrullus lanatus var. citroides: Role of reactive nitrogen species and cyclooxygenase enzyme inhibition

Cited by 95 publications

References 24 publications

Anti-Inflammatory Mechanisms of the Annexin A1 Protein and Its Mimetic Peptide Ac2-26 in Models of Ocular Inflammation In Vivo and In Vitro

Anti-Inflammatory Mechanisms of the Annexin A1 Protein and Its Mimetic Peptide Ac2-26 in Models of Ocular Inflammation In Vivo and In Vitro

Protective effect of juice extract of mesocarp rind of Citrullus lanatus in carbon tetrachloride-induced oxidative stress in rats

Evaluation of Anti-inflammatory and Antioxidant Properties of Crude Extract and Forskolin from Solena amplexicaulis Leaf

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