Anti-inflammative effect of glycyrrhizin on rat thermal injury via inhibition of high-mobility group box 1 protein

Shen, Liangyun; Cui, Ziwei; Lin, Yunfeng; Wang, Shuqin; Zheng, Dongfeng; Tan, Qian

doi:10.1016/j.burns.2014.05.008

Cited by 25 publications

(16 citation statements)

References 48 publications

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“…48 Reduction of IL-1β by GLY treatment also has been shown in a noninfectious mouse model of acute glaucoma 49 and in rats with severe thermal injury. 50 …”

Section: Discussionmentioning

confidence: 99%

Glycyrrhizin Reduces HMGB1 and Bacterial Load in Pseudomonas aeruginosa Keratitis

Ekanayaka

McClellan

Barrett

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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PurposeHigh mobility group box 1 (HMGB1) contributes to poor disease outcome in Pseudomonas aeruginosa keratitis. This study tests the prophylactic effect of treatment with HMGB1 inhibitors, glycyrrhizin (GLY) and its derivative, carbenoxolone (CBX), for Pseudomonas keratitis.MethodsWe treated C57BL/6 (B6) mice subconjunctivally with GLY or CBX, infected with a noncytotoxic clinical isolate (KEI 1025) or a cytotoxic strain (ATCC 19660) of P. aeruginosa, and injected intraperitoneally with either agent. Clinical score, photography with a slit lamp, real-time RT-PCR, ELISA, myeloperoxidase (MPO) assay, bacterial plate count, histopathology, and absorbance assays were used to assess treatment efficacy and bacteriostatic activity.ResultsAfter KEI 1025 infection, GLY treatment reduced HMGB1 (mRNA and protein levels) and improved disease outcome with significant reduction in mRNA levels of IL-1β, TLR4, CXCL2, and IL-12; protein expression (IL-1β, CXCL2); neutrophil infiltrate; and bacterial load. Treatment with GLY enhanced antimicrobial proteins, including CRAMP and mBD2, but not mBD3. Glycyrrhizin also reduced clinical scores and improved disease outcome in corneas infected with strain 19660. However, neither HMGB1 mRNA or protein levels were reduced, but rather, CXCL2 expression (mRNA and protein), neutrophil infiltrate, and bacterial load were reduced statistically. Treatment with GLY initiated 6 hours after infection reduced plate count; GLY also was bacteriostatic for KEI 1025 and ATCC 19660.ConclusionsGlycyrrhizin reduces HMGB1 and is protective against P. aeruginosa–induced keratitis with a clinical isolate that is noncytotoxic. It was similar, but less effective when used after infection with a cytotoxic strain, which did not reduce HMGB1.

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“…48 Reduction of IL-1β by GLY treatment also has been shown in a noninfectious mouse model of acute glaucoma 49 and in rats with severe thermal injury. 50 …”

Section: Discussionmentioning

confidence: 99%

Glycyrrhizin Reduces HMGB1 and Bacterial Load in Pseudomonas aeruginosa Keratitis

Ekanayaka

McClellan

Barrett

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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“…50 Furthermore, at 48 h postburn in a rat model of 30% TBSA (no fluid resuscitation), secondary tissue damage was indicated by elevated TNF-a and IL-1b in serum, as well as elevated HMGB1 mRNA and protein in lung, liver, and kidney. 51 …”

Section: Organ Dysfunction In Severe Burnsmentioning

confidence: 99%

Toll-Like Receptor Signaling in Burn Wound Healing and Scarring

D’Arpa

Leung

2017

Advances in Wound Care

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“…This mediator affect in angiogenesis and wound healing [36]. Shen et al, in their studies showed that administration of Gly before and after burn via intraperitoneal significantly improved thermal skin injury [35]. Gly, with reduction in serum inflammatory factors (TNF-a, IFN-γ, IL-6, IL-1b , and IL-17 ) and the increase in HMGB1 protein/mRNA expression from damaged cells is effective in wound healing [36].…”

Section: Discussionmentioning

confidence: 99%

“…HMGB1, as an early mediator of inflammation, increase in traumatic injury and plays a cytokine-like in burn injury [35]. This mediator affect in angiogenesis and wound healing [36].…”

Section: Discussionmentioning

confidence: 99%

Licorice cream promotes full-thickness wound healing in Guinea pigs

Hanafi¹,

Amiri²,

Shahani³

et al. 2018

jrp

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Impaired wound healing may cause economic and social problems and seriously reduce the quality of life. The uses of herbal medicines as new alternative treatments are under investigation. This study investigated the effect of Glycyrrhiza glabra L. extract on the full-thickness wound healing in Guinea Pig model. Eight male Guinea Pigs (700-800 g) were used to be creared a square full-thickness wound with 1.5 ×1.5 dimensions on the lumbodorsal area. The wounds were randomly divided into four groups: I; control, II; 1% phenytoin, III; 5% G. glabra and IV; 10 % G.glabra. On day 3, 5, 7, 9 and 12, wound size was measured for assessment of the percent of wound healing. In order to determine the wound healing activity, excisional biopsies were evaluated histopathologically on the 12th days of treatments. Acute inflammation, granulation tissue fibroblast maturation, collagen deposition, epidermal layer formation, neovascularization, keratin layer formation were evaluated according to the Abramov score method. Hydroalcoholic extract of G.glabra exhibited total phenolic and flavonoid contents of 114.1 ± 5.45 and 82.85 ± 6.38 mg, respectively. G.glabra creams (5% and 10% w/w)were significantly increased the epidermal formation, collagen deposition and neovascularization, and was decreased acute inflammation in comparison to the control group. Wound healing rate were increased in the G. glabra groups. G.glabra creams 10 % was more effective than 5% w/w. Our findings proved that 5% and 10% w/w G. glabra creams were effective in acute dermal wound healing. More studies with different doses of G. glabra extract are recommended.

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Anti-inflammative effect of glycyrrhizin on rat thermal injury via inhibition of high-mobility group box 1 protein

Cited by 25 publications

References 48 publications

Glycyrrhizin Reduces HMGB1 and Bacterial Load in Pseudomonas aeruginosa Keratitis

Glycyrrhizin Reduces HMGB1 and Bacterial Load in Pseudomonas aeruginosa Keratitis

Toll-Like Receptor Signaling in Burn Wound Healing and Scarring

Licorice cream promotes full-thickness wound healing in Guinea pigs

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