Anti-infective activity of Cratylia argentea lectin (CFL) against experimental infection with virulent Listeria monocytogenes in Swiss mice

Santana, Lucas Nunes; Tavares, Lethicia Souza; Dorvigny, Betty Mancebo; Souza, Francisco de Assis Leite; Paiva, Bruno Henrique de Albuquerque; Evêncio-Neto, Joaquim; Hounkonnou, Soke Gninlome Cedril; Silva, Ayrles Fernanda Brandão; Ramos, Márcio V.; Lima‐Filho, José Vitor

doi:10.1016/j.phymed.2021.153839

Cited by 2 publications

(3 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Antibacterial activity exhibited by ESL in the present study was quite impressive as legume lectins were toxic to bacterial pathogens. This is attributable to interactions involving inherent aromatic amino acids and hydrogen bonding between hydroxyl oxygen of sugars and lectin CRD (Dias et al, 2015 ; El-Araby et al, 2020 ; Santana et al, 2022 ). Santana et al ( 2022 ) showed that lectin isolated from the tropical legume Cratylia argentea elicited potent toxicity toward Listeria monocytogenes by reducing its systemic loads.…”

Section: Resultsmentioning

confidence: 99%

“…This is attributable to interactions involving inherent aromatic amino acids and hydrogen bonding between hydroxyl oxygen of sugars and lectin CRD (Dias et al, 2015 ; El-Araby et al, 2020 ; Santana et al, 2022 ). Santana et al ( 2022 ) showed that lectin isolated from the tropical legume Cratylia argentea elicited potent toxicity toward Listeria monocytogenes by reducing its systemic loads. El-Araby et al ( 2020 ) showed that purified lentil and pea lectins inhibited the growth of S. aureus and P. aeruginosa , with inhibition zones of 35 and 33.4 mm, respectively, as against 20 and 22 nm manifested by ESL in the present study.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Antimicrobial Activities and Phylogenetic Study of Erythrina senegalensis DC (Fabaceae) Seed lectin

Enoma¹,

Adewole²,

Agunbiade³

et al. 2023

bta

View full text Add to dashboard Cite

Erythrina senegalensis (Fabaceae) have been traditionally used in the treatment of microbial ailments, and the specific agent mediating its efficacy has been investigated in several studies. In this study, the antimicrobial activity of purified E. senegalensis lectin (ESL) was analyzed. The phylogenetic relationship of the gene encoding lectin with other legume lectins was also established to investigate their evolutionary relationship via comparative genomics. Antimicrobial activity of ESL against selected pathogenic bacteria and fungi isolates was evaluated by the agar well diffusion method, using fluconazole (1 mg/ml) and streptomycin (1 mg/ml) as positive controls for fungi and bacteria sensitivity, respectively. Potent antimicrobial activity of ESL against Erwinia carotovora, Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus, Aspergillus niger, Penicillium camemberti, and Scopulariopsis brevicaulis was observed, with inhibition zones ranging from 18 to 24 mm. Minimum inhibitory concentrations of ESL ranged between 50 and 400 μg/ml. Primer-directed polymerase chain reaction of E. senegalensis genomic DNA detected a 465-bp lectin gene with an open reading frame encoding a 134-amino acid polypeptide. The obtained nucleotide sequence of the ESL gene shared high sequence homology: 100, 100, and 98.18% with Erythrina crista-galli, Erythrina corallodendron, and Erythrina variegata lectin genes, respectively, suggesting that the divergence of Erythrina lectins might follow species evolution. This study concluded that ESL could be used to develop lectin-based antimicrobials, which could find applications in the agricultural and health sectors.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Antimicrobial Activities and Phylogenetic Study of Erythrina senegalensis DC (Fabaceae) Seed lectin

Enoma¹,

Adewole²,

Agunbiade³

et al. 2023

bta

View full text Add to dashboard Cite

show abstract

“…Samples of spleen and the liver were fixed in 10% formalin, buffered with sodium phosphate solution, pH 7.2, for 24 hr (Santana et al, 2021). The tissues were dehydrated in an ethyl alcohol series and then cleared with xylol for 1 hr.…”

Section: Methodsmentioning

confidence: 99%

Antiinflammatory and antiinfective effect of caffeine in a mouse model of disseminated salmonellosis

Dorvigny

Tavares

Almeida

et al. 2021

Phytotherapy Research

Self Cite

View full text Add to dashboard Cite

Caffeine has been reported for its antiinflammatory properties by stimulating phagocytosis. In this study, we investigated the antiinflammatory and antiinfective potential of caffeine in murine macrophage cell cultures and Swiss mice infected with virulent Salmonella enterica serotype typhimurium. Peritoneal macrophages (pMØ) were treated with caffeine on 96-well plates for 24 hr and then infected with Salmonella for 4 hr. In another experiment, the pMØ were first infected with the bacterium for 4 hr and then treated with caffeine for 24 hr. In addition, Swiss mice were inoculated, intraperitoneally, with S. typhimurium and then received caffeine intravenously. Control groups received phosphate-buffered saline (PBS) or dexamethasone. We found that treatments with caffeine increased the macrophage cell viability and reduced the intracellular bacterial load. The administration of caffeine to Swiss mice reduced the infiltration of leukocytes into the peritoneal cavity after the bacterial challenge.Furthermore, the bacterial burdens in the peritoneal fluid, bloodstream, spleen, and liver were decreased by caffeine treatment. The expression levels of tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β), IL-6, and inducible nitric oxide synthase (iNOs) were down-regulated after infection in caffeine-treated mice. We can conclude that caffeine has both antiinflammatory and antiinfective properties that can be useful for management of bacterial infections along with antibiotics.

show abstract

Anti-infective activity of Cratylia argentea lectin (CFL) against experimental infection with virulent Listeria monocytogenes in Swiss mice

Cited by 2 publications

References 29 publications

Antimicrobial Activities and Phylogenetic Study of Erythrina senegalensis DC (Fabaceae) Seed lectin

Antimicrobial Activities and Phylogenetic Study of Erythrina senegalensis DC (Fabaceae) Seed lectin

Antiinflammatory and antiinfective effect of caffeine in a mouse model of disseminated salmonellosis

Contact Info

Product

Resources

About