Anti-Helicobacter pylori activity and Essential Oil Composition of Thymus caramanicus from Iran

Abstract

The essential oil of the dried aerial parts of Thymus caramanicus at full flowering stage was prepared by hydrodistillation with a yield of 2.5% oil. The oil analysis by a combination of capillary GC and GC-MS revealed 26 components of which carvacrol (68.9%) was the main component, followed by p-cymene (6.0%), thymol (5.3%), γ-terpinene (4.6%) and borneol (4.0%) representing 98.9% of the total oil. The in vitro antibacterial activity of the essential oil was determined against ten clinical isolates of Helicobacter pylori using disc diffusion, as well as measurement of minimum inhibitory concentrations. The results showed high inhibitory activity against all test bacteria by the disc diffusion method (zones of inhibition of 50.0-65.0 mm). Minimum inhibitory concentration values were within the range 14.5-58.0 μg/mL for the clinical isolates.

Binary and Tertiary Mixtures of Satureja hortensis and Origanum vulgare Essential Oils as Potent Antimicrobial Agents Against Helicobacter pylori

“…The anti‐ H . pylori potential of carvacrol has been proven in several studies (Bergonzelli et al ., ; Eftekhar et al ., ; Falsafi et al ., ), and our findings are in accordance with them. On the contrary, monoterpene hydrocarbons, sabinene and γ‐terpinene, did not express any inhibitory activity in concentration up to 2.05 mg mL −1 .…”

“…Thus, the further search for the most efficient essential oil based formulation against H . pylori is justified (Bergonzelli et al ., ; Deriu et al ., ; Eftekhar et al ., ; Esmaeili et al ., ; Falsafi et al ., ; Ohno et al ., ; Ozen et al ., ).…”

“…However, there are numerous reports on anti-H. pylori activity of various essential oils. Thus, the further search for the most efficient essential oil based formulation against H. pylori is justified (Bergonzelli et al, 2003;Deriu et al, 2007;Eftekhar et al, 2009;Esmaeili et al, 2012;Falsafi et al, 2015;Ohno et al, 2003;Ozen et al, 2014).…”

Volatile Constituents of Two Rare Subspecies of Thymus praecox

“…It is believed that part of these activities is due to the volatile constituents. In recent years, the essential oil chemistry and polymorphism of various Thymus species have been extensively investigated [3][4][5][6][7]. These studies have emphasized the existence of marked chemical differences among oils extracted from different species or varieties.…”

Helicobacter Pylori Infection and Its Relevant to Chronic Gastritis

“…Aqueous extract of Glucyrrhiza -globra L (Fabaceae) 1mg /ml significantly inhibited the adhesion of HP to human stomach tissue. The effect was related to the polysaccarIdes isolated from the extract (Eftekhar et al, 2009)…”