Anti-<i>Campylobacter</i>activity of resveratrol and an extract from waste Pinot noir grape skins and seeds, and resistance of<i>Camp. jejuni</i>planktonic and biofilm cells, mediated via the CmeABC efflux pump

Klančnik, Anja; Pogačar, Maja Šikić; Trošt, Kajetan; Žnidarič, Magda Tušek; Vodopivec, Branka Mozetič; Možina, Sonja Smole

doi:10.1111/jam.13315

Cited by 66 publications

(51 citation statements)

References 38 publications

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“…These were then incubated at the test temperatures. For determination of viable adhered (at 4 h) and biofilm cells (at 24 and 72 h), CFU plate counting was used . To take into account the surface areas, the numbers of viable cells were calculated as log CFU cm −2 .…”

Section: Methodsmentioning

confidence: 99%

“…To take into account the surface areas, the numbers of viable cells were calculated as log CFU cm −2 . For biomass (at 24 and 72 h), crystal violet assay was used, using 0.1% crystal violet dye (Merck, Darmstadt, Germany) and absorbance measured at 584 nm . The absorbances (A) were standardized according to the Biofilm Production Index (BPI), calculated according to Eqn (3):

BPI = (\frac{A}{surface}) \times 1000

…”

Section: Methodsmentioning

confidence: 99%

“…To determine viable adhered cells and biofilm formation of the P. fragi and P. psychrophila spoilage bacteria and the L. monocytogenes and E. coli pathogenic bacteria in monospecies and multispecies cultures on polystyrene, CFU plate counting was used after 4 h of adhesion and after 24, 72, and 168 h of biofilm formation. The standardized monospecies or multispecies inocula were prepared (each spoilage bacterium with both pathogenic bacteria) in TSB or in the fish meat model and transferred to microplate wells (200 μL), incubated at 5 °C, and analyzed as described previously . Biofilm cells were prepared from the adhered monospecies and multispecies bacteria, inoculated onto selective agars and determined as log CFU mL −1 .…”

Section: Methodsmentioning

confidence: 99%

“…For determination of viable adhered (at 4 h) and biofilm cells (at 24 and 72 h), CFU plate counting was used. 15 To take into account the surface areas, the numbers of viable cells were calculated as log CFU cm −2 . For biomass (at 24 and 72 h), crystal violet assay was used, using 0.1% crystal violet dye (Merck, Darmstadt, Germany) and absorbance measured at 584 nm.…”

Section: Adhesion Biofilm and Biomass Of P Fragi And P Psychrophimentioning

confidence: 99%

“…For biomass (at 24 and 72 h), crystal violet assay was used, using 0.1% crystal violet dye (Merck, Darmstadt, Germany) and absorbance measured at 584 nm. 11,15 wileyonlinelibrary.com/jsfa…”

Section: Adhesion Biofilm and Biomass Of P Fragi And P Psychrophimentioning

confidence: 99%

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Spoilage Pseudomonas biofilm with Escherichia coli protection in fish meat at 5 °C

2019

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BACKGROUND Pseudomonas are part of the indigenous microbiota of different foods, where they gradually cause spoilage. In fish meat, Pseudomonas fragi and Pseudomonas psychrophila have been identified as important spoilers. The initial aim of this study was to investigate the physiological characteristics, adhesion, and biofilm of P. fragi and P. psychrophila under temperatures related to the fish‐processing industry. The further aim was to define the problem of increased growth of pathogenic bacteria in the presence of spoilage bacteria in vitro and in fish meat. RESULTS Temperature dependence on physiological characteristics, adhesion, and biofilm was observed. Hydrophobicity and autoaggregation were most prominent at 15 °C, and at this temperature floating biofilm was also formed. The adhesion of these Pseudomonas was up to 2 log CFU cm−1 more pronounced on stainless steel than polystyrene, with up to five times greater biofilm biomass production at 5 °C on polystyrene. This paralleled at least a 0.5 log CFU g−1 increase in the pathogenic bacterium Escherichia coli in fish meat. CONCLUSION Pseudomonas fragi and P. psychrophila adhesion and biofilm depend on the temperature, and are stimulated by temperatures that can occur during the processing and storage of fish meat. Strong Pseudomonas biofilm formation under refrigeration conditions is protective for E. coli, potentially by providing more favorable conditions by ensuring a higher concentration of nutrients. Interactions between spoilage Pseudomonas and pathogenic bacteria can occur through different mechanisms, and an understanding of these is of particular importance to ensure the overall quality and safety of fish meat and other proteinaceous foods. © 2019 Society of Chemical Industry

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Section: Methodsmentioning

confidence: 99%

BPI = (\frac{A}{surface}) \times 1000

…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Adhesion Biofilm and Biomass Of P Fragi And P Psychrophimentioning

confidence: 99%

“…For biomass (at 24 and 72 h), crystal violet assay was used, using 0.1% crystal violet dye (Merck, Darmstadt, Germany) and absorbance measured at 584 nm. 11,15 wileyonlinelibrary.com/jsfa…”

Section: Adhesion Biofilm and Biomass Of P Fragi And P Psychrophimentioning

confidence: 99%

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