Anti Hyperglycaemic and Antioxidant Activity of Catunaregam Spinosa (Thunb) Against Dexamethasone Induced Diabetes in Rats

Abstract

Objective:The study was aimed at evaluating the folkloric use of the Catunaregam spinosa (Thunb) bark for its hypoglycaemic and antioxidant activity against dexamethasone-induced diabetes in wistar rats. Methods:Diabetes was induced in wistar rats by dexamethasone administration 10 mg/kg, b. wt., s. c for 11 consecutive days to all group animals (except Group I). After confirmation of diabetes, the animals were divided into 5 groups (n=6). Group I: Vehicle control treated with normal saline only, Group II: Diabetic control treated with dexamethasone only, Group III: Standard control treated with dexamethasone plus glibenclamide (5 mg/kg, b. wt., p. o), Group IV and V: Test control received dexamethasone plus ethanolic extract of Catunaregam spinosa (EECS) at graded doses of 200 mg/kg, b. wt. and 400 mg/kg, b. wt., p. o for 21days respectively. On the last day of the experiment, the effect of Catunaregam spinosa bark was measured by estimating the biochemical and antioxidant parameters. Results:There was a significant alteration in the serum glucose, HbA1c, total proteins, serum lipid profile and tissue antioxidant parameters in dexamethasone-treated animals when compared to the normal control rats.In test groups treated with glibenclamide and EECS, it was significantly ameliorated the altered parameters such as serum glucose, HbA1c, total proteins, serum lipid profile and the antioxidant parameters like SOD, CAT, GSH and MDA when compared to dexamethasone-treated animals with evidence of histopathological studies of the pancreas. Conclusion:The results indicated that the ethanolic extract of Catunaregam Spinosa (Thunb) bark has shown the hypoglycemic and antioxidant property.

α-Amylase Inhibitory Activity of Catunaregam spinosa (Thunb.) Tirveng.: In Vitro and In Silico Studies

“…The in vitro α -amylase inhibition analysis was reported for the first time from this plant. This can be related to the previously reported in vivo analysis of the antihyperglycemic activities of methanolic extract of C. spinosa fruit extract in the nicotinamide and streptozotocin-induced male Wistar diabetic rats of which C. spinosa fruit extract (400 mg/kg) decreased the blood sugar level from 106.16 ± 7.8 to 87.5 ± 5.00 mg/dL [ 18 ] and in stem bark extract (400 mg/kg) treated group to 94.00 ± 1.577 mg/dL as compared to the diabetic control group [ 9 ].…”

“…This plant has been reported from various parts of China, Nepal, Bangladesh, and India [ 8 ]. Traditionally, it has been used to treat various symptoms such as skin diseases, tumors, wounds, ulcers, and diabetes [ 6 , 9 ]. In India, the bark juice of this plant is used to treat several gastrointestinal problems such as diarrhea and dysentery [ 10 ].…”