Anti-human versus anti-guinea pig tissue transglutaminase antibodies as the first-level serological screening test for coeliac disease in the general population

Fabiani, Elisabetta; Peruzzi, E.; Mandolesi, Alessandra; Garbuglia, G.; Fanciulli, Giuseppe; D’Appello, A.R.; Gasparin, Maurizio; Bravi, E.; Bearzi, Italo; Galeazzi, Renato L.; Catassi, Carlo

doi:10.1016/j.dld.2004.05.008

Cited by 20 publications

(19 citation statements)

References 32 publications

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“…The above observation could be confirmed when human recombinant tissue transglutaminase was used as an antigen -mean titres of IgA were significantly higher in psoriatics than in controls (but only in 7.4% of cases, titres of a-h-r-tTG IgA were higher than the 90th percentile of the control values). Fabiani et al 26 proved that the anti-tissue transglutaminase specificity and sensitivity were 98% and 92% for guinea pig and 99.6% and 96% for human recombinant tissue transglutaminase, respectively. Authors suggest that the anti-human tissue transglutaminase test should definitely replace the anti-guinea pig-derived one as first-level screening tool.…”

Section: Discussionmentioning

confidence: 99%

Serologic markers of celiac disease in psoriatic patients

Damasiewicz-Bodzek¹,

Wielkoszyński

2008

Acad Dermatol Venereol

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Section: Discussionmentioning

confidence: 99%

Serologic markers of celiac disease in psoriatic patients

Damasiewicz-Bodzek¹,

Wielkoszyński

2008

Acad Dermatol Venereol

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“…[14][15][16] As these are IgA-class antibodies, false-negative results occur in IgA-deficiency which itself is associated with CD. 17 Patients with IgA-deficiency should be tested with IgG-class anti-tTG 17 or EmA, 18 or be offered distal duodenal biopsy.…”

Section: Diagnosis Of and Screening Tools For CDmentioning

confidence: 99%

Celiac Disease and Autoimmune Thyroid Disease

Ch’ng¹,

Jones²,

Kingham³

2007

Clinical Medicine & Research

150

104

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Cel iac disease (CD) or gluten sensitive enteropathy is a permanent intolerance of dietary gluten leading to mucosal damage in the proximal small bowel in genetically susceptible individuals, characterized by inflammation, crypt hyperplasia and villous atrophy which regress on withdrawal of gluten from the diet. Recent population screening studies have shown the prevalence of CD in Western countries approaches 1% 1-3 but the condition is greatly under-diagnosed, partly because many cases are subclinical, but also because of its previously perceived rarity.CD should be considered in many clinical settings and detected early to prevent complications in later life. In a 6-year prospective study, Corrao et al 4 followed 1,072 consecutive CD patients and found a standard mortality ratio of 0.5 in those who complied with a strict gluten-free diet but a standard mortality ratio of 6.0 in the poor compliers. Using the general practice research database, West et al 5 identified 4,732 CD patients and found a modest increase in overall risks of malignancy and mortality compared to matched controls. 184Celiac disease (CD) or gluten sensitive enteropathy is relatively common in western populations with prevalence around 1%. With the recent availability of sensitive and specific serological testing, many patients who are either asymptomatic or have subtle symptoms can be shown to have CD. Patients with CD have modest increases in risks of malignancy and mortality compared to controls. The mortality among CD patients who comply poorly with a gluten-free diet is greater than in compliant patients.The pattern of presentation of CD has altered over the past three decades. Many cases are now detected in adulthood during investigation of problems as diverse as anemia, osteoporosis, autoimmune disorders, unexplained neurological syndromes, infertility and chronic hypertransaminasemia of uncertain cause.Among autoimmune disorders, increased prevalence of CD has been found in patients with autoimmune thyroid disease, type 1 diabetes mellitus, autoimmune liver diseases and inflammatory bowel disease. Prevalence of CD was noted to be 1% to 19% in patients with type 1 diabetes mellitus, 2% to 5% in autoimmune thyroid disorders and 3% to 7% in primary biliary cirrhosis in prospective studies. Conversely, there is also an increased prevalence of immune based disorders among patients with CD.The pathogenesis of co-existent autoimmune thyroid disease and CD is not known, but these conditions share similar HLA haplotypes and are associated with the gene encoding cytotoxic T-lymphocyte-associated antigen-4.Screening high risk patients for CD, such as those with autoimmune diseases, is a reasonable strategy given the increased prevalence.Treatment of CD with a gluten-free diet should reduce the recognized complications of this disease and provide benefits in both general health and perhaps life expectancy. It also improves glycemic control in patients with type 1 diabetes mellitus and enhances the absorption of medications for associated hypoth...

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“…Using human recombinant tTG as antigen, Fabiani et al demonstrated a prevalence of subclinical CD of 0.7% (25/3541) in the general Italian population (19). Using human recombinant tTG as antigen, Fabiani et al demonstrated a prevalence of subclinical CD of 0.7% (25/3541) in the general Italian population (19).…”

Section: Discussionmentioning

confidence: 99%

“…We found a high prevalence of tTGA positivity among patients with peripheral nervous system disorders or MND AIDP, acute inflammatory demyelinating polyneuropathy; ANA, anti-nuclear antibodies; aPL, anti-phospholipid; ASMA, anti-smooth muscle antibodies; DSP, distal symmetric polyneuropathy; MND, motor neuron disease; tTGA, anti-tissue transglutaminase antibodies. Moreover, we used human recombinant tTG as antigen, which has been demonstrated to offer higher specificity than that observed with the use of the guineapig liver-derived tTG (19). The problem of the so-called Ôfalse positiveÕ tTGA reactivity has been recently tackled by Villalta et al (20), who attributed this event to methodological unreliability for samples with high immunoglobulin concentration.…”

Section: Discussionmentioning

confidence: 99%