Anti-human immunodeficiency virus (anti-HIV) natural products with special emphasis on HIV reverse transcriptase inhibitors

Ng, T.B.; Huang, Boshi; Fong, Wing‐Ping; Yeung, H.W.

doi:10.1016/s0024-3205(97)00245-2

Cited by 134 publications

(68 citation statements)

References 53 publications

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“…Some flavonoids work on the intracellular replication of viruses, whereas others inhibit the infectious properties of the viruses. Flavonoids have inhibitory activity on reverse RT and RNA-directed DNA polymerase [94,95]; however, it also has antiintegrase and antiprotease activities [96]. Similarly, myricetin activity was tested against HIV-RT and inhibited the enzyme by 49 % [97]…”

Section: Flavonoidsmentioning

confidence: 99%

Oxidative Stress, Redox Regulation and Elite Controllers of HIV Infection: Towards a Functional Cure

Okechukwu¹

2015

Trends in Basic and Therapeutic Options in HIV Infection - Towards a Functional Cure

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Section: Flavonoidsmentioning

confidence: 99%

Oxidative Stress, Redox Regulation and Elite Controllers of HIV Infection: Towards a Functional Cure

Okechukwu¹

2015

Trends in Basic and Therapeutic Options in HIV Infection - Towards a Functional Cure

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“…42 A number of Chinese herbal extracts have also been tested in vitro and show promise, 43 though a recent meta-analysis of existing data proved inconclusive and suggested that larger and more rigorous trials were needed. 44 The results for all three HIV targets are presented in Table 7.…”

Section: C Cox and Loxmentioning

confidence: 99%

Virtual Screening of Chinese Herbs with Random Forest

Ehrman

Barlow

Hylands

2007

J. Chem. Inf. Model.

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Random Forest, a form of multiple decision trees, has been used to screen a database of Chinese herbal constituents for potential inhibitors against several therapeutically important molecular targets. These comprise cyclic adenosine 3′-5′-monophosphate phosphodiesterases, protein kinase A, cyclooxygenases, lipoxygenases, aldose reductase, and three HIV targets-integrase, protease, and reverse transcriptase. In addition, compounds were identified which may inhibit the expression of inducible nitric oxide synthase and/or nitric oxide production in vivo. A total of 240 Chinese herbs containing 8264 compounds were screened in silico, including many used on a regular basis in traditional Chinese medicine. Active compounds were selected from another database of 2597 phytochemicals and related natural products with known target affinities and covered a wide range of structural classes. Random Forest was found to perform well, even on highly unbalanced data characteristic of ligand-based screening where the compounds to be screened are far more numerous than the number of active compounds used in training. Despite a conservative screening protocol, a wide variety of compounds from Chinese herbs were hit. Of particular interest were the relatively large number of herbs predicted to inhibit multiple targets, as well as a number which appeared to contain inhibitors of the same target from different phytochemical classes. The latter point to the possibility that individual species may make use of alternative phytochemical strategies in target inhibition. A literature search provided evidence to support 83 herb-target predictions.

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“…They include alkaloids, sulphated polysaccharides, polyphenolics, flavonoids, coumarins, phenolics, tannins, triterpenes, lectins, phloroglucinols, lactones, iridoids, depsidones, O-caffeoyl derivatives, lignans, and ribosome inactivating proteins, saponins, xanthones, naphthodianthrones, photosensitisers, phospholipids, quinines and peptides [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Hiv-1 Reverse Transcriptase and Protease Assay of Methanolic Extracts of Adansonia Digitata L.

Sharma

Rangari

2016

Int J Pharm Pharm Sci

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Objective: Anti-HIV activity studies of methanolic extracts of Adansonia digitata L. leaves, root bark and fruit pulp. Methods:Methanolic extracts of A. digitata were tested for HIV enzyme inhibitory activity against HIV-1 reverse transcriptase (RT) and HIV-1 protease (PR). HIV-1 RT assay was performed using non-radioactive HIV-RT colorimetric ELISA kit while the HIV-1 PR assay was performed using a fluorogenic octapeptide substrate, HIV-FRET (fluorescence resonance energy transfer) and a recombinant HIV-1 protease solution (AnaSpec Inc., USA). Results:The percentage inhibition of controls and A. digitata leaves, root bark and fruit pulp extracts were calculated relative to uninhibited HIV-1 RT and PR in 2% DMSO. The results of the HIV-1 RT assay indicated 26.5% inhibition by root bark extract (50 µg/ml) and 12.2% inhibition by leaf extract while the fruit pulp extract (100 µg/ml) has shown only 5.9% inhibition as compared to standard Nevirapine (100 nM) 27.5% inhibition.In the case of HIV-protease assay, leaf extract and fruit pulp extract (50 µg/ml) has shown quite high inhibition (≥ 50%) to the extent of 75% and 74% respectively which is very significant. The root bark extract has shown 35% inhibition. Conclusion:Methanolic extract of the leaves, root bark and fruit pulp of A. digitata has shown low anti-HIV-1 RT but significant PR activity. HIV-1 RT activity of root bark extract and HIV-1 PR activity of fruit pulp extract indicates the potential of the plant as an anti-HIV agent.

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Anti-human immunodeficiency virus (anti-HIV) natural products with special emphasis on HIV reverse transcriptase inhibitors

Cited by 134 publications

References 53 publications

Oxidative Stress, Redox Regulation and Elite Controllers of HIV Infection: Towards a Functional Cure

Oxidative Stress, Redox Regulation and Elite Controllers of HIV Infection: Towards a Functional Cure

Virtual Screening of Chinese Herbs with Random Forest

Hiv-1 Reverse Transcriptase and Protease Assay of Methanolic Extracts of Adansonia Digitata L.

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