Anti-Helicobacter pylori flavonoids from licorice extract

Fukai, Toshio; Marumo, Ai; Kaitou, Kiyoshi; Kanda, Toshihisa; Terada, Shohei; Nomura, Taro

doi:10.1016/s0024-3205(02)01864-7

Cited by 319 publications

(209 citation statements)

References 40 publications

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“…and Fukai et al [9] demonstrated that compounds extracted from G. glabra and G. uralensis have good antibacterial activity against Helicobacter pylori. Fukai et al [10] determined that 19 flavonoids isolated from G. glabra, G. inflata and G. uralensis were active against methycilline sensitive Staphylococcus aureus (MSSA), MRSA, M. luteus, B. subtilis, E. coli, K. pneumoniae and P. aeruginosa.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Antimicrobial and antioxidant activities of medicinal plant Glycyrrhiza glabra var. glandulifera from different habitats

Karahan

Avşar

Özyiğit

et al. 2016

Biotechnology & Biotechnological Equipment

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In this study, the antimicrobial and antioxidant activities of root methanolic extracts of Glycyrrhiza glabra var. glandulifera (Waldst. & Kit.) Boiss. (Fabaceae) were investigated. Plant samples were collected from different habitats in the East Mediterranean part of Turkey. The plant extracts were evaluated for antimicrobial activities against nine bacterial and two yeast strains using discdiffusion and minimum inhibitory concentration methods. The antioxidant activity was determined by using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. The antimicrobial assays indicated that the plant root extracts were more effective against Gram-positive bacteria than against Gramnegative ones. In addition, the extracts had higher antimicrobial effect against Candida species than against bacteria. The extracts showed good antioxidant activity, with a median inhibitory concentration (IC 50 ) in the range of 588 § 0.86 mg/mL to 2190 § 1.73 mg/mL. Results indicated that different environmental conditions in each habitat might affect the contents of chemical compounds and biological activity in the natural licorice populations of. This study also supported the traditional use of licorice and as well as suggested that it may also be its beneficial role in the treatment of other infections. The obtained results indicated that different environmental conditions in each habitat might affect the contents of chemical compounds and the biological activity in the natural licorice populations.

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Section: Antimicrobial Activitymentioning

confidence: 99%

Antimicrobial and antioxidant activities of medicinal plant Glycyrrhiza glabra var. glandulifera from different habitats

Karahan

Avşar

Özyiğit

et al. 2016

Biotechnology & Biotechnological Equipment

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“…Licorice is one of the most commonly prescribed herbs in Chinese Traditional Medicine, and has been used for >2,000 years. The effects of licorice have been reported on various diseases, ranging from microbial infection to cancer (5)(6)(7). However, since herbs usually contain numerous chemical compositions, the mechanism of action of these herbs is currently unclear.…”

Section: Introductionmentioning

confidence: 99%

Licochalcone A induces T24 bladder cancer cell apoptosis by increasing intracellular calcium levels

Yang

Jiang

Yang

et al. 2016

Molecular Medicine Reports

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Abstract. Licochalcone A (LCA) has been reported to significantly inhibit cell proliferation, increase reactive oxygen species (ROS) levels, and induce apoptosis of T24 human bladder cancer cells via mitochondria and endoplasmic reticulum (ER) stress-triggered signaling pathways. Based on these findings, the present study aimed to investigate the mechanisms by which LCA induces apoptosis of T24 cells. Cultured T24 cells were treated with LCA, and cell viability was measured using the sulforhodamine B assay. Apoptosis was detected by flow cytometry with Annexin V/propidium iodide staining, and by fluorescent microscopy with Hoechst 33258 staining. The levels of intracellular free calcium ions were determined using Fluo-3 AM dye marker. Intracellular ROS levels were assessed using the 2' ,7'-dichlorodihydrofluorescein diacetate probe assay. The mitochondrial membrane potential was measured using 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl benzimidazole carbocyanine iodide. Furthermore, the mRNA expression levels of B-cell lymphoma (Bcl)-extra large, Bcl-2-associated X protein, Bcl-2-interacting mediator of cell death, apoptotic protease activating factor-1 (Apaf-1), calpain 2, cysteinyl aspartate specific proteinase (caspase)-3, caspase-4 and caspase-9 were determined using reverse transcription semiquantitative and quantitative polymerase chain reaction analyses. Treatment with LCA inhibited proliferation and induced apoptosis of T24 cells, and increased intracellular Ca 2+ levels and ROS production. Furthermore, LCA induced mitochondrial dysfunction, decreased mitochondrial membrane potential, and increased the mRNA expression levels of Apaf-1, caspase-9 and caspase-3. Exposure of T24 cells to LCA also triggered calpain 2 and caspase-4 activation, resulting in apoptosis. These findings indicated that LCA increased intracellular Ca 2+ levels, which may be associated with mitochondrial dysfunction. In addition, the ER stress pathway may be considered an important mechanism by which LCA induces apoptosis of T24 bladder cancer cells. IntroductionNatural herbal medicines have long been used to treat cancer. Biomedical research and cancer treatment clinical trials have provided evidence regarding the use of herbal medicines; therefore, they are increasingly being accepted as a complementary and alternative treatment (1). In addition, natural medicines have been reported to have an important role in human health, particularly certain well-studied plants, including Taxus chinensis (Taxus madia) (2), Radix Sophorae flavescentis (Sophora flavescens) (3), Alkanna tinctoria, Lithospermum erythrorhizon and licorice (Glycyrrhiza L) (4). Licorice is one of the most commonly prescribed herbs in Chinese Traditional Medicine, and has been used for >2,000 years. The effects of licorice have been reported on various diseases, ranging from microbial infection to cancer (5-7). However, since herbs usually contain numerous chemical compositions, the mechanism of action of these herbs is currently unclear. Recently, several chemical ing...

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“…On the contrary, evidence-based functional foods, in combination with lifestyle modification, tive properties, 5) and its clinical effects in moderately hypercholesterolemic patients have been reported. 6) Glabridin, a major polyphenolic flavonoid of G. glabra, has anti-oxidative, [7][8][9][10][11] antiHelicobacter pylori, 12) as well as anti-nephritic and radical scavenging activities, 13) and inhibits serotonin re-uptake, 14) melanogenesis and inflammation. 15) However, an anti-obese effect of licorice has not previously been reported.…”

Section: Introductionmentioning

confidence: 99%

Licorice Flavonoid Oil Effects Body Weight Loss by Reduction of Body Fat Mass in Overweight Subjects

Tominaga

Mae

Kitano

et al. 2006

JOURNAL OF HEALTH SCIENCE

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Licorice flavonoid oil (LFO) is a new dietary ingredient for functional foods consisting of licorice hydrophobic polyphenols in medium-chain triglycerides (MCT). In an effective dose finding study conducted previously, LFO has exhibited a dose-dependent body fat-reducing effect. Here we report the weight-reducing effect of LFO in a placebo-controlled, double-blind, long-term (12 weeks) ingestion study at 300 mg/day, the minimal effective dose observed in the dose finding study. A total of 103 overweight subjects [body mass index (BMI): 24-30] completed this study and were analyzed. Body weight increased in the placebo group, but was maintained at close to preingestion level in the LFO group, resulting in significant (p < 0.05) differences in the changes in body weight and BMI between the LFO group and the placebo group at each time-point. Dual-energy X-ray absorptiometry (DXA) measurement of body fat indicated that the weight-reducing effect was attributable to reduced body fat. No clinically significant adverse events occurred during the 12-week ingestion period. To confirm the safety of LFO for practical use we also conducted a placebo-controlled, double-blind safety study in 40 overweight subjects with a 4-week excessive ingestion at 1800 mg/day; 6 times the dose of the 300 mg/day study that exhibited a weight-reducing effect. No clinically significant adverse events occurred during the 4-week ingestion period. Based on these findings in both human studies it was shown that LFO is a safe ingredient for functional foods even for long-term or excessive ingestion, with a potential weight-reducing effect.

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Anti-Helicobacter pylori flavonoids from licorice extract

Cited by 319 publications

References 40 publications

Antimicrobial and antioxidant activities of medicinal plant Glycyrrhiza glabra var. glandulifera from different habitats

Antimicrobial and antioxidant activities of medicinal plant Glycyrrhiza glabra var. glandulifera from different habitats

Licochalcone A induces T24 bladder cancer cell apoptosis by increasing intracellular calcium levels

Licorice Flavonoid Oil Effects Body Weight Loss by Reduction of Body Fat Mass in Overweight Subjects

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