Anti-feline panleukopenia virus serum neutralizing antibody titer in domestic cats with the negative or low hemagglutination inhibition antibody titer

Abstract

To evaluate the accuracy of hemagglutination inhibition (HI) test as the index of feline panleukopenia virus (FPV)-protective ability, sera from 153 FPV-vaccinated cats aged ≥7 months with HI titer of <1:10–1:40, were examined for serum neutralizing (SN) antibody. SN antibody was detected (≥1:10) in 33 (62.3%) of 53 HI antibody-negative cats, and ranged <1:10–1:160. This suggests that FPV-antibody detection sensitivity of HI test is lower than SN test, and SN test is more suitable for the assessment of FPV-vaccine effect than HI test especially in cats with negative or low HI titer. SN titer was 1:32, FPV-protective threshold, or higher in all cats with HI titers of ≥1:20, suggesting it may be appropriate to set protective HI threshold at 1:20.

Evaluation of an in-house indirect enzyme-linked immunosorbent assay of feline panleukopenia VP2 subunit antigen in comparison to hemagglutination inhibition assay to monitor tiger antibody levels by Bayesian approach

“…ELISA and HI tests have been developed to assess antibody titers against feline parvovirus infections in cats [ 21 , 22 ]. However, the specificity of the serum neutralizing (SN) test is the highest among viral serologic testing [ 23 ]. Since the SN test is considered time-consuming and costly, the HI test is being widely used to determine antibody titer against FPV [ 14 , 23 – 25 ].…”

“…However, the specificity of the serum neutralizing (SN) test is the highest among viral serologic testing [ 23 ]. Since the SN test is considered time-consuming and costly, the HI test is being widely used to determine antibody titer against FPV [ 14 , 23 – 25 ]. The ELISA test has been recognized as a sensitive and reliable method to determine humoral antibody response against parvovirus, possibly allowing for the calculation of the time that vaccinations can be performed [ 14 , 26 ].…”

Imunização de gatos frente aos Retrovírus da Imunodeficiência Felina (FIV) e Leucemia Felina (FeLV)

“…Em contrapartida, Soma et al (2019) estudou títulos de anticorpos neutralizantes de FPV em 718 gatos com HI negativa ou baixa no período de 2013 a 2017, mostrando que o desempenho vacinal pode ter sido subestimado utilizando esta técnica e que gatos com HI negativa ou baixa deveriam ter uma contra prova com SN. A especificidade do teste sorológico considerado padrão ouro, é a mais alta entre os testes sorológicos.…”

Preparation and Preliminary Application of Fluorescent Microsphere Test Strips for Feline Parvovirus Antibodies

“…The HI test is considered the gold standard for detecting FPV antibodies [5]. Generally, an HI antibody titre of 1:20 to 1:40 is considered protective against FPV [6,7].…”