Anti-Escherichia coli activity of Brazilian plant extracts: New trends in Veterinary research

Camargo, Livia Roberta Piedade; Suffredini, Ivana Barbosa

doi:10.1590/1678-41626072

Cited by 4 publications

(5 citation statements)

References 6 publications

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“…In recent decades, our researchers have been systematically developing projects aiming to identify antimicrobial active extracts from the Amazon and Atlantic Brazilian rainforests. We have studied more than 2,000 plant extracts and essential oils as antimicrobials against Staphylococcus aureus (Pinto, 2020), Staphylococcus epidermidis (Belo, 2022), Enterococcus faecalis (Castilho, 2009), Streptococcus mutans (Bento, 2020), Streptococcus sanguinis (Silva, 2009), Escherichia coli (Camargo, 2016), Pseudomonas aeruginosa, Candida albicans (Rudiger, 2021), Malassezia pachydermatis (Silva, 2018), Malassezia furfur ( Silva, 2018 ), and Listeria monocytogenes (Ceruso et al, 2020), which are shown in the present article as a result of a 15-year study in antimicrobial-active natural product identification (Suffredini et al, 2002;Suffredini et al, 2004;Suffredini et al, 2006a;Suffredini et al, 2006b;Younes et al, 2007;Castilho et al, 2013;Camargo and Suffredini, 2014;Castilho et al, 2014;da Silva et al, 2014;Costa et al, 2016;Suffredini et al, 2016;Martins et al, 2019a;Martins et al, 2019b;Camargo et al, 2020;Silva et al, 2020).. The present article reports a step-by-step methodology for identifying antimicrobial products in nature that comprises plant collection, the different tests used to analyze the in vitro antimicrobial activity, a test to determine toxicity, and a statistical-based algorithm to classify the extracts according to their potential.…”

Section: Introductionsupporting

confidence: 59%

“…To optimize the costs of the random-chemotaxonomic plant collection strategy, small amounts of plant material are collected in the field, resulting in small amounts of plant extracts, so it is vital to adjust the in vitro assay scales to the use of micrograms of plant extracts A proposed ranking of 111 plant extracts according to their antimicrobial activity efficacy and low toxicity. Suffredini et al 10.3389/frabi.2023.1122400 Frontiers in Antibiotics frontiersin.org (Suffredini et al, 2002;Suffredini et al, 2004;Suffredini et al, 2006a;Suffredini et al, 2006b;Younes et al, 2007;Castilho et al, 2013;Camargo and Suffredini, 2014;Castilho et al, 2014;da Silva et al, 2014;Costa et al, 2016;Suffredini et al, 2016;Martins et al, 2019a;Martins et al, 2019b;Camargo et al, 2020;Silva et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

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Screening Amazon rainforest plant extracts for antimicrobial activity: a 15-year commitment to the Brazilian biodiversity

Suffredini,

Silva,

Frana

et al. 2023

Front. Antibiot.

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IntroductionThe need for new tools to treat infections is constantly growing due to the possibilities of emerging diseases related to environmental changes, climatic catastrophes, microorganism resistance, and human and animal aging, leading to an evident unbalance in the planet’s health. Brazil contains the most significant portion of world biodiversity, a potential source of new antimicrobial natural products. Nonetheless, its environment, particularly its forests, and rainforests, is under threat, meaning that rapidly conducted, comprehensive research into the potential of antimicrobial activity to address this threat is urgently needed.MethodsIn this study, plants from the Amazon rainforest and the Atlantic forests were collected and tested against several pathogenic microbes relevant to humans, animals, and the environment, and subjected to large-scale susceptibility assays, bioautography, and Artemia salina toxicity assays. From the plants, 2,280 organic and aqueous extracts were obtained from different organs, namely leaves, barks, flowers, fruits, and seeds, and subjected to a large-scale susceptibility screening assay against Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Streptococcus mutans, Streptococcus sanguinis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Malassezia pachydermatis, Malassezia furfur, and Listeria monocytogenes. Results and discussionThe selected extracts were subjected to antimicrobial susceptibility tests to determine their inhibition zone diameters and minimum bactericidal concentrations, to bioautography, and to an Artemia salina toxicity assay, which resulted in 154 active extracts. Moreover, 111 out of 154 extracts were ranked based on scores established by the p-values and the mean rank differences in each set of test results. The final ranking identified which extracts should be studied in further phytochemical research using thin-layer chromatography techniques as a priority. The extracts obtained from plants belonging to Combretaceae, Connaraceae, Convolvulaceae, Fabaceae, Malpighiaceae, Moraceae, Piperaceae, Polygonaceae, and Salicaceae were selected as the most promising ones and used to support the identification of plant-based antimicrobial active compounds from the immense biodiversity of Brazilian forests.

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Section: Introductionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Screening Amazon rainforest plant extracts for antimicrobial activity: a 15-year commitment to the Brazilian biodiversity

Suffredini,

Silva,

Frana

et al. 2023

Front. Antibiot.

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“…E. coli 51A, characterized by a virulent and non-resistant profile, was classified in the B2 phylogenetic group, and was named ONT:H7. E. coli ATCC ® 25922™ was used in the initial screening made with more than 1300 plant extracts obtained from the Amazon Forest, which resulted in four active extracts, being EB127 one of them [13]. For a more accurate and detailed antimicrobial analysis, the three strains of E. coli that were obtained from frigate's cloaca were introduced in the analysis, to verify whether the antimicrobial potential of the plant extract would be the same, or if it would be specific to each kind of micro-organism, considering their characteristics of virulence and resistance.…”

Section: Discussionmentioning

confidence: 99%

“…Loganic acid (L) was identified in the sample named UN-365, which was obtained from the 10%ACN/H 2 O fraction, by CC, analytical thin-layer chromatography and preparative thin-layer chromatography. Loganic acid was identified by means of 1 H-NMR and 13 C-NMR [29] (Table -5).…”

Section: Loganic Acidmentioning

confidence: 99%

“…The study aimed at the biological and chemical investigation of the organic extract obtained from the stem of Microplumeria anomala (Apocynaceae), EB127, as it was identified as a potential source of new antibacterial compounds to be used in Veterinary. The extract was previously selected in a high-throughput screening [ 13 ] to be chemically and biologically studied, and to have active compounds identified. M. anomala is the only species within its genus, Microplumeria Baill.…”

Section: Introductionmentioning

confidence: 99%

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Susceptibility of virulent and resistant Escherichia coli strains to non-polar and polar compounds identified in Microplumeria anomala

et al. 2020

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Background and Aim: Escherichia coli is one of the main pathogens responsible for veterinary and human infections, and it is associated with significant economic losses in the livestock, as it causes severe diseases to humans, particularly in children. For that reason, there is a need for introducing new drugs to treat E. coli diseases. The Brazilian species richness is a source of potential new antibacterial natural products. The study aimed at the biological and chemical investigation of the organic extract obtained from the stem of Microplumeria anomala (Apocynaceae), EB127, as it was identified as a potential source of new antibacterial compounds to be used in Veterinary. Materials and Methods: The antibacterial activity was evaluated by disk diffusion and microdilution assays; chromatography, nuclear magnetic resonance spectrometry, and mass spectrometry were used in the isolation and identification of compounds. Results: EB127 showed activity against E. coli ATCC25922, and against three E. coli strains that were isolated from frigarte's cloaca, named 31/1A, 35A, and 51A. Lupeol, 3-acetyl-11-oxo-β-amyrin, 3-acetyl-11-oxo-α-amyrin, sitosterol, stigmasterol, 3β,7α-dihydroxy-cholest-5-ene, 3β-hydroxy-cholest-5-en-7-one, and 3β-hydroxy-cholest-5,22-dien-7-one were identified in fraction Hex/CHCl3, while loganin, loganic acid, methylanomaline, and anomaline were all identified in EB127 and protocatechuic acid hexoside, ferulic acid, secoxyloganin, feruloylquinic acid, vanillic acid hexoside, protocatechuic acid-4-O-β-hexoside, and rosmarinic acid were tentatively identified in fraction 10%ACN/H2O. E. coli 51A (virulent/non-resistant) showed sensitivity to the antibacterial action of fraction Hex/CHCl3 which contains alkaloids, triterpenes, and steroids, while E. coli 35A (resistant/non-virulent) were more susceptible to 10%ACN/H2O, which contains iridoids as loganin and loganic acid, and glycosylated and non-glycosylated caffeic acids. Conclusion: Fraction 10%ACN/H2O is of interest in pursuing new drugs to treat resistant E. coli, in veterinary. All compounds were isolated from the plant for the first time and have shown potential as new antibacterial natural products from Amazon plants to be used in veterinary and human diseases.

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Alkaloids and biological activity of beribá (Annona hypoglauca)

Rinaldi

Díaz

Suffredini

et al. 2017

Revista Brasileira de Farmacognosia

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Anti-Escherichia coli activity of Brazilian plant extracts: New trends in Veterinary research

Cited by 4 publications

References 6 publications

Screening Amazon rainforest plant extracts for antimicrobial activity: a 15-year commitment to the Brazilian biodiversity

Screening Amazon rainforest plant extracts for antimicrobial activity: a 15-year commitment to the Brazilian biodiversity

Susceptibility of virulent and resistant Escherichia coli strains to non-polar and polar compounds identified in Microplumeria anomala

Alkaloids and biological activity of beribá (Annona hypoglauca)

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