The primary objective of this study is to evaluate the seed extracts from agro waste to examine the effectiveness of antimicrobial. Therefore, by using the disc diffusion assay, the antibacterial activity was evaluated along with two medically important bacterial pathogens such as methicillin resistant Staphylococcus aureus (S. aureus) (MRSA) and Escherichia coli (E. coli) in the seed extracts of Cucurbita sp., Carica papaya L., Nephelium lappaceum, Mangifera indica, Durio zibethius and Artocarpus heterphyllus Lam seeds. Seed extracts of agro waste were analyzed for the phytochemical constituents. Analysis of the qualitative phytochemical indicated the importance of phytochemical compounds for instance glycosides, saponins, flavanoids, tannins and alkaloids. Variations had been seen in the inhibition zone depending on the species and different seed extracts concentrations used for bacteria, the highest inhibition zone observed for M. indica and C. papaya L. The obtained data of this study presented greater insights which allow the use of seed extracts that were obtained from the agro-waster, to be used as raw materials for food, chemical, and pharmaceutical industries.