Anti-epiligrin cicatricial pemphigoid and epidermolysis bullosa acquisita: Differentiation by use of indirect immunofluorescence microscopy

U-serrated immunodeposition pattern differentiates type VII collagen targeting bullous diseases from other subepidermal bullous autoimmune diseases

“…The sections were examined with a Leica DMRA microscope for selective incident light fluorescence 8 . For observation of FITC fluorescence (blue narrow‐band excitation) we used an XBO75W lamp and a Chroma high Q filter set containing: (i) exciter filter HQ 480/40; (ii) dichroic beam splitter Q 505 LP; and (iii) emitter filter HQ 535/50.…”

Orf-induced pemphigoid with antilaminin-332 antibodies

“…The combination of a linear n‐serrated deposition pattern in patient skin and a dermal binding of antibodies on salt‐split skin suggests an antilaminin‐332 mucous membrane pemphigoid or a p200 pemphigoid 7 . To elucidate this further we used knockout skin substrates, lacking either laminin‐332 from a patient with Herlitz junctional epidermolysis bullosa, or lacking type VII collagen from a patient with recessive dystrophic epidermolysis bullosa 8 . Both substrates were incubated with patient serum.…”

Detection of collagen VII autoantibodies to NC1 and NC2 domains of collagen VII by ELISA in suspected epidermolysis bullosa acquisita and bullous lupus erythematosus patients

“…Tests on skin deficient in type VII collagen gave negative or reduced staining in 8/14 group I patients, hence confirming the presence of collagen VII autoantibodies ( Fig. If positive BMZ reactions are seen on normal skin by IIF, further tests on collagen VII deficient substrates can help differentiate between these diseases [3]. 7/8 of these patients had ELISA unit values greater than the cut off value (median 82.4 U/ml).…”