Anti‐Diabetic Potential of Panax Notoginseng Saponins (PNS): A Review

Uzayisenga, Rosette; Ayeka, Peter Amwoga; Wang, Yi

doi:10.1002/ptr.5026

Cited by 100 publications

(55 citation statements)

References 75 publications

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“…These properties are expected to be useful for modulating those phenomena in diseases. In particular PNS could be applied to treat impaired angiogenesis and lymphangiogenesis of diabetes mellitus (Kononenkov et al, 2014; Lee et al, 2007; Liu et al, 2015; Uzayisenga et al, 2014; Wang et al, 2013). …”

Section: Discussionmentioning

confidence: 99%

Total saponins of panaxnotoginseng promotes lymphangiogenesis by activation VEGF-C expression of lymphatic endothelial cells

Chen

Zhang

et al. 2016

Journal of Ethnopharmacology

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Ethnopharmacological relevance Lymphatic system plays an important role in maintaining the fluid homeostasis and normal immune responses, anatomic or functional obstruction of which leads to lymphedema, and treatments for therapeutic lymphangiogenesis are efficiency for secondary lymphedema. Total saponins of panaxnotoginseng (PNS) are a mixture isolated from Panaxnotoginseng (Burkill) F.H. Chen, which has been used as traditional Chinese medicine in China for treatment of cardio- and cerebro-vascular diseases. The aim of this study was to determine the effect and mechanism of PNS on lymphangiogenesis. Methods The Tg (fli1:egfp; gata1:dsred) transgenic zebrafish embryos were treated with different concentrations of PNS (10, 50, 100μM) for 48 hours with or without the 6 hours pretreatment of the 30μM Vascular endothelial growth factors receptor (VEGFR)-3 kinase inhibitor, followed with morphological observation and lympangiogenesis of thoracic duct assessment. The effect of PNS on cell viability, migration, tube formation and Vascular endothelial growth factors (VEGF)-C mRNA and protein expression of lymphatic endothelial cells (LECs) were determined. The role of phosphatidylinositol-3 (PI-3)-kinase (PI3K), extracellular signal-regulated kinase (ERK)1/2 pathways, c-Jun N-terminal kinase (JNK) and P38 mitogen activated protein kinases (MAPK) signaling in PNS-induced VEGF-C expression of LECs by using pharmacological agents to block each signal. Results PNS promotes lymphangiogenesis of thoracic duct in zebrafish with or without VEGFR3 Kinase inhibitor pre-impairment. PNS promotes proliferation, migration and tube formation of LECs. The tube formation induced by PNS could be blocked by VEGFR3 Kinase inhibitor. PNS induce VEGF-C expression of LEC, which could be blocked by ERK1/2, PI3K and P38MAPK signaling inhibitors. Conclusion PNS activates lymphangiogenesis both in vivo and in vitro by up-regulating VEGF-C expression and activation of ERK1/2, PI3K and P38MAPK signaling. These findings provide a novel insight into the role of PNS in lymphangiogenesis and suggest that it might be an attractive and suitable therapeutic agent for treating secondary lymphedema or other lymphatic system impairment related disease.

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Section: Discussionmentioning

confidence: 99%

Total saponins of panaxnotoginseng promotes lymphangiogenesis by activation VEGF-C expression of lymphatic endothelial cells

Chen

Zhang

et al. 2016

Journal of Ethnopharmacology

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“…Many studies have been reported that PNS might be a potential treatment choice for various diseases. Recent studies have found that the hypoglycemic and anti-obesity properties of PNS may play an important role in the treatment of diabetes [3]. PNS injection has been reported may provide another choice for patients with angina pectoris (AP), although application of PNS alone showed no significant better or worse effect on AP patients, evidence showed PNS combined with traditional western medicine was a better treatment option for AP patient in improving patients’ clinical symptoms [4].…”

Section: Introductionmentioning

confidence: 99%

Effects of panax notoginseng saponins on the osteogenic differentiation of rabbit bone mesenchymal stem cells through TGF-β1 signaling pathway

Wang

Huang

Tang

et al. 2016

BMC Complement Altern Med

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BackgroundPanax Notoginseng is a well-known Chinese medicinal herb which has been used in China for treatment of bone fracture for hundreds of years. However, the specific biological mechanisms of osteogenic effect of PNS are not well understood.MethodsIn this study, newborn rabbit BMSCs were isolated, and then identified by the positive expression rates of cell surface markers, including CD29, CD45 and HLA-DR, which were detected by flow cytometry(FCM). After the lentivirus-induced cell model of TGF-β1 gene silencing was established, the interference efficiency was tested by q-PCR and Western blot, and the growth curve of silencing cells was drawn by MTT so as to grasp the growth rhythm of silencing cells. In the alizarin red-staining experiment, the effect of 100 mg/L PNS on the activity of intracellular ALP of TGF-β1 gene silencing BMSCs was detected, so as to observe the effect of 100 mg/L PNS on the formation of calcium nodes of gene silencing BMSCs.ResultsBy separating rabbit BMSCs, the lentivirus-induced cell model of TGF-β1 gene silencing was established. Both TGF-β1 mRNA and protein expression were restrained significantly, and the target gene kept silence stably via the verification of q-PCR and Western blot; there was no significant differences of the growth curve between RNAi cells and normal cells; the activity of intracellular APL in 100 mg RNAi group was obviously lower than that in 100 mg group (p < 0.05), but higher than that in the normal group; in the alizarin red-staining experiment, it focused on the effects of PNS on the formation of calcium nodes of gene silencing BMSCs, which showed that calcium nodes could be formed in 100 mg RNAi group but its quantity was lower than that of 100 mg group (p < 0.05).ConclusionsIt was shown that silencing TGF-β1 gene could interrupt the osteogenic effects of PNS. PNS may have a promoting effect on osteogenic differentiation of rabbits’ BMSCs in vitro by up-regulating the gene expression of TGF-β1.

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“…Some of its various medicinal properties are anti-inflammatory, antioxidative, therapeutic opportunities in cardiovascular diseases, anti-diabetic and neuroprotective (Yang et al 2015;Zhou et al 2014;Liu et al 2014;Uzayisenga et al 2014;Choi et al 2010). Despite its valuable properties, frequent outbreaks of root rot disease in the plantation are a major hindrance in its production.…”

Section: Introductionmentioning

confidence: 99%

Flavobacterium notoginsengisoli sp. nov., isolated from the rhizosphere of Panax notoginseng

Zhang

et al. 2015

Antonie van Leeuwenhoek

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Two novel bacterial strains, designated SYP-B540(T) and SYP-B556, were isolated from rhizospheric soil of Panax notoginseng located at Yunnan Province, China. Both strains were Gram-staining negative, aerobic, non-motile, elongated rod shaped and yellow coloured. They grew optimally at 28 °C and pH 7.0. Analysis of 16S rRNA gene sequences showed that the two strains shared 99.8 % sequence similarity to each other, but lower than 97.6 % to the other known species of the genus Flavobacterium. The predominant respiratory quinone for the two strains was MK-6, and the major fatty acids were iso-C15:0 and summed Feature 3 (comprising 16:1 ω7c and/or 16:1 ω6c). The polar lipids consisted of phosphatidylethanolamine, two unidentified polar lipids and three unidentified amino-phospholipids. The DNA G+C contents of strains SYP-B540(T) and SYP-B556 were 33.3 and 32.7 mol%, respectively. In addition, the DNA-DNA hybridization values of strains SYP-B540(T) and SYP-B556 to their closest phylogenetic neighbors were significantly lower than 70 %. On the basis of the polyphasic taxonomy studies, strains SYP-B540(T) and SYP-B556 represent a novel species of the genus Flavobacterium, for which the name Flavobacterium notoginsengisoli sp. nov. is proposed. The type strain is SYP-B540(T) (=KCTC 32505(T) = NBRC 110012(T) = BCRC 80724(T)).

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Anti‐Diabetic Potential of Panax Notoginseng Saponins (PNS): A Review

Cited by 100 publications

References 75 publications

Total saponins of panaxnotoginseng promotes lymphangiogenesis by activation VEGF-C expression of lymphatic endothelial cells

Total saponins of panaxnotoginseng promotes lymphangiogenesis by activation VEGF-C expression of lymphatic endothelial cells

Effects of panax notoginseng saponins on the osteogenic differentiation of rabbit bone mesenchymal stem cells through TGF-β1 signaling pathway

Flavobacterium notoginsengisoli sp. nov., isolated from the rhizosphere of Panax notoginseng

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