Anti-diabetic effect of isoflavone rich kudzu root extract in experimentally induced diabetic rats

Duru, Kingsley C.; Mukhlynina, Elena; Moroz, Gleb A.; Гетте, И. Ф.; Данилова, И. Г.; Kovaleva, Elena G.

doi:10.1016/j.jff.2020.103922

Cited by 18 publications

(8 citation statements)

References 44 publications

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“…The oral glucose tolerance test (OGTT) is an experimental method for diagnosing diabetes. 55 The test results can reflect the body's ability to regulate blood glucose. The OGTT results (Fig.…”

Section: Effect On the Body Weight Fbg Ogtt And Itt In T2dm Micementioning

confidence: 99%

Glycosides and flavonoids from the extract of Pueraria thomsonii Benth leaf alleviate type 2 diabetes in high-fat diet plus streptozotocin-induced mice by modulating the gut microbiota

Zhang

Guo

et al. 2022

Food Funct.

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Section: Effect On the Body Weight Fbg Ogtt And Itt In T2dm Micementioning

confidence: 99%

Glycosides and flavonoids from the extract of Pueraria thomsonii Benth leaf alleviate type 2 diabetes in high-fat diet plus streptozotocin-induced mice by modulating the gut microbiota

Zhang

Guo

et al. 2022

Food Funct.

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“…The final mixtures were heated at 60–80 °C under constant stirring until transparent solutions were obtained. The prepared NADESs were stored in the dark until further use [ 33 ].…”

Section: Methodsmentioning

confidence: 99%

Antioxidant and Cytotoxic Activities of Kudzu Roots and Soy Molasses against Pediatric Tumors and Phytochemical Analysis of Isoflavones Using HPLC-DAD-ESI-HRMS

Aboushanab

Шевырин

Slesarev

et al. 2022

Plants

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Pediatric solid tumors (PSTs) are life-threatening and can lead to high morbidity and mortality rates in children. Developing novel remedies to treat these tumors, such as glioblastoma multiforme and sarcomas, such as osteosarcoma, and rhabdomyosarcoma, is challenging, despite immense attempts with chemotherapeutic or radiotherapeutic interventions. Soy (Glycine max) and kudzu roots (KR) (Pueraria spp.) are well-known phytoestrogenic botanical sources that contain high amounts of naturally occurring isoflavones. In the present study, we investigated the antioxidant and cytotoxic effects of the extracts of KR and soy molasses (SM) against PSTs. The green extraction of isoflavones from KR and SM was performed using natural deep eutectic solvents. The extracts were subsequently analyzed by high-performance liquid chromatography (HPLC)-diode array detector (DAD) coupled with high-resolution (HR) mass spectrometry (MS), which identified 10 isoflavones in KR extracts and 3 isoflavones in the SM extracts. Antioxidant and cytotoxic activities of KR and SM extracts were assessed against glioblastoma multiforme (A-172), osteosarcoma (HOS), and rhabdomyosarcoma (Rd) cancer cell lines. The KR and SM extracts showed satisfactory cytotoxic effects (IC50) against the cancer cell lines tested, particularly against Rd cancer cell lines, in a dose-dependent manner. Antioxidant activity was found to be significantly (p ≤ 0.05) higher in KR than in SM, which was consistent with the results of the cytotoxic activity observed with KR and SM extracts against glioblastoma and osteosarcoma cells. The total flavonoid content and antioxidant activities of the extracts were remarkably attributed to the isoflavone content in the KR and SM extracts. This study provides experimental evidence that HPLC-ESI-HRMS is a suitable analytical approach to identify isoflavones that exhibit potent antioxidant and anticancer potential against tumor cells, and that KR and SM, containing many isoflavones, can be a potential alternative for health care in the food and pharmaceutical industries.

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“…However, very often DESs cannot be evaporated due to the high viscosity and extremely low volatility of their components. When necessary, target compounds can be recovered from DESs using macroporous resin column chromatography (e.g., LX-38, AB-8, HPD-450, HP-20) [15,16,22]. Thus, the obtained extracts are passed through a prehydrated column and after adsorption, the loaded column is first washed with sufficient deionized water until all the DESs are washed away, then the phenolic compounds can be eluted with a small volume of the most appropriate organic solvent (e.g., methanol).…”

Section: Dess For the Extraction Of Bioactive Compounds From Plants Fruit And Vegetablesmentioning

confidence: 99%

Deep Eutectic Solvents for the Extraction of Bioactive Compounds from Natural Sources and Agricultural By-Products

et al. 2021

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In this work, a review about the applicability of eutectic solvents, mainly deep eutectic solvents (DES) and natural deep eutectic solvents (NADES), for the extraction of bioactive compounds from natural products has been carried out. These alternative solvents have shown not only to have high extraction yields but also to be environmentally friendly, exhibiting very low or almost no toxicity, compared to conventional organic solvents. The last trends and main extraction methods that have been most widely used in studies using these emerging solvents have been reviewed, as well as the varied natural sources in which they have been used, including agro-food by-products. Besides the toxicity, biodegradability of these solvents is reviewed. Likewise, different reported bioactivity tests have been included, in which extracts obtained with these ecological solvents have been tested from antioxidant activity analysis to in vivo studies with rats, through in vitro cytotoxicity tests.

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Anti-diabetic effect of isoflavone rich kudzu root extract in experimentally induced diabetic rats

Cited by 18 publications

References 44 publications

Glycosides and flavonoids from the extract of Pueraria thomsonii Benth leaf alleviate type 2 diabetes in high-fat diet plus streptozotocin-induced mice by modulating the gut microbiota

Glycosides and flavonoids from the extract of Pueraria thomsonii Benth leaf alleviate type 2 diabetes in high-fat diet plus streptozotocin-induced mice by modulating the gut microbiota

Antioxidant and Cytotoxic Activities of Kudzu Roots and Soy Molasses against Pediatric Tumors and Phytochemical Analysis of Isoflavones Using HPLC-DAD-ESI-HRMS

Deep Eutectic Solvents for the Extraction of Bioactive Compounds from Natural Sources and Agricultural By-Products

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