Anti-diabetic effect of hesperidin on palmitate (PA)-treated HepG2 cells and high fat diet-induced obese mice

Rajan, Priyanka; Natraj, Premkumar; Ranaweera, Sachithra S.; Dayarathne, Lakshi A.; Lee, Young Jae; Han, Chang-Hoon

doi:10.1016/j.foodres.2022.112059

Cited by 9 publications

(5 citation statements)

References 39 publications

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“…Tannic acid is currently being researched as an organic polymer additive owing to its bioactive characteristics and its ability to improve the capabilities of materials for biomedical applications [ 89 ]. By restoring the normal expression levels of the genes related to insulin signaling and glucose metabolism that were disturbed in the liver of high-fat-diet-induced obese mice, hesperidin has the potential to have an antidiabetic effect [ 90 ].…”

Section: Discussionmentioning

confidence: 99%

Determination of Antioxidant, Anti-Alzheimer, Antidiabetic, Antiglaucoma and Antimicrobial Effects of Zivzik Pomegranate (Punica granatum)—A Chemical Profiling by LC-MS/MS

Karageçili

İzol

Kıreçcı

et al. 2023

Life

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Zivzik pomegranate (Punica granatum) has recently sparked considerable interest due to its nutritional and antioxidant properties. To evaluate the antioxidant capacities of P. granatum juice, ethanol (EEZP), and water (WEZP) extracts from peel and seed, the antioxidant methods of 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid radical (ABTS•+) scavenging, 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH•) scavenging, Fe3+-2,4,6-tris(2-pyridyl)-S-triazine (TPTZ) reducing, Fe3+ reducing, and Cu2+ reducing methods were used. The antioxidant capacities of samples were compared with the most commonly used synthetic antioxidants, i.e., BHA, BHT, α-tocopherol, and Trolox. In terms of setting an example, the IC50 values of EEZP for ABTS•+ and DPPH• scavenging activities were found to be lower than standards, at 5.9 and 16.1 μg/mL, respectively. The phenolic and flavonoid contents in EEZP peel were 59.7 mg GAE/g and 88.0 mg QE/g, respectively. Inhibition of α-glycosidase, α-amylase, acetylcholinesterase, and human carbonic anhydrase II (hCA II) enzymes was also investigated. EEZP demonstrated IC50 values of 7.3 μg/mL against α-glycosidase, 317.7 μg/mL against α-amylase, 19.7 μg/mL against acetylcholinesterase (AChE), and 106.3 μg/mL against CA II enzymes. A total of 53 phenolic compounds were scanned, and 30 compounds were determined using LC-MS/MS. E. coli and S. aureus bacteria were resistant to all four antibiotics used as standards in hospitals.

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Section: Discussionmentioning

confidence: 99%

Determination of Antioxidant, Anti-Alzheimer, Antidiabetic, Antiglaucoma and Antimicrobial Effects of Zivzik Pomegranate (Punica granatum)—A Chemical Profiling by LC-MS/MS

Karageçili

İzol

Kıreçcı

et al. 2023

Life

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“…Western blotting was conducted as per the methods described in refs and . The primary antibodies that used targeted AMPK, ACC, p-AMPK (Thr172), and p-ACC (Ser79) were sourced from Cell Signaling Technology.…”

Section: Methodsmentioning

confidence: 99%

“…The 2-[ N -(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amino]-2-deoxy- d -glucose (2-NBDG) uptake assay was utilized to evaluate glucose uptake in 3T3-L1 adipocytes. , Preadipocytes were seeded in 96-well plates and subjected to differentiation as detailed in Figure B. Following a 12 h serum deprivation, they were exposed to the sample treatments for 24 h. Subsequently, these cells were incubated with 40 μM 2-NBDG (CA) at 37 °C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Antiadipogenic Effect of Citrus Flavonoids: Evidence from RNA Sequencing Analysis and Activation of AMPK in 3T3-L1 Adipocytes

Natraj,

Rajan,

Jeon

et al. 2023

J. Agric. Food Chem.

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Citrus fruits are rich in dietary flavonoids and have many health benefits, but their antiadipogenic mechanism of action and their impact on lipid metabolism remain unclear. In this study, we investigated the effect of citrus flavonoids, namely, hesperidin (HES), narirutin (NAR), nobiletin (NOB), sinensetin (SIN), and tangeretin (TAN), on preventing fat cell development by gene expression in 3T3-L1 adipocytes. Among the citrus flavonoids tested, HES and NAR significantly reduced fat storage and triglyceride levels and increased glucose uptake in 3T3-L1 adipocytes. Additionally, HES and NAR treatment increased the phosphorylation of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) while reducing the protein expression of 3-hydroxy-3-methylglutaryl CoA reductase (HMGCR). Furthermore, in silico docking revealed that flavonoids activate AMPK. RNA sequencing analysis demonstrated that citrus flavonoids normalized the expression of 40 genes, which were either upregulated by more than 2-fold or downregulated by less than 0.6-fold including Acadv1,

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“…( 8 , 9 ). Mounting evidence has demonstrated that flavanones exert multiple therapeutic effects including anti-inflammation ( 10 , 11 ), antioxidant ( 12 , 13 ), anti-cancer ( 14 , 15 ), cardioprotection ( 16 , 17 ), anti-diabetic ( 18 ), and anti-obesity ( 19 , 20 ). Vernarelli et al ( 21 ) found that in U.S. adults, intake of flavonoids, including flavanones, was inversely associated with Ob and C-reactive protein levels (a marker of inflammation) in women.…”

Section: Introductionmentioning

confidence: 99%

“…Studies in Korean adults have found that a high intake of flavanones may be associated with a decreased body fat percentage and CO in women ( 22 ). In addition, several animal experimental studies have found that hesperidin, naringenin, and eriodictyol can improve lipid metabolism and control body weight and Ob ( 10 , 18 , 19 , 23 , 24 ). To our knowledge, however, most of the current studies have focused on adults and animals, and no studies on the association of flavanones and its subclasses, with Ow/Ob and CO in children and adolescents.…”

Section: Introductionmentioning

confidence: 99%

Association between intake of flavanones and the overweight/obesity and central obesity in children and adolescents: a cross-sectional study from the NHANES database

Liu,

2024

Front. Nutr.

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AimThe prevalence of obesity (Ob), overweight (Ow) and central obesity (CO) in children and adolescents has increased dramatically over the past decades globally. Flavanones have been recently studied as adjuvants for the treatment of obesity. This study was aimed at evaluating the association between intake of flavanones and its subclasses and the Ow/Ob and CO in children and adolescents.MethodsThis cross-sectional study extracted the data of children and adolescents with Ow/Ob and CO from the National Health and Nutrition Examination Survey (NHANES) database for 2007–2010 and 2017–2018. Ow and Ob were defined as a body mass index (BMI) ≥ 85th percentile. CO was defined as a waist circumference (WC) ≥ 90th percentile. The association between intake of flavanones and its subclasses and the Ow/Ob and CO in children and adolescents was determined by weighted univariate and multivariate Logistic regression models adjusted for potential covariates, and odds ratios (ORs) with 95% confidence intervals (CIs) was calculated. To further explore association between intake of flavanones and its subclasses and the Ow/Ob and CO in children and adolescents, subgroup analyses stratified by age, and gender.ResultsOf the total 5,970 children and adolescents, 2,463 (41.2%) developed Ow/Ob and 1,294 (21.7%) patients developed CO. High intake of flavanones, eriodictyol, hesperetin, and naringenin were associated with lower odds of Ow/Ob in children and adolescents. (OR: 0.75, 95%CI: 0.62–0.92, OR: 0.69, 95%CI: 0.55–0.87, OR: 0.69, 95%CI: 0.55–0.87, and OR: 0.76, 95%CI: 0.63–0.92, respectively). In addition, high intake of flavanones, eriodictyol, and naringenin were associated with lower odds of CO in children and adolescents (OR: 0.71, 95%CI: 0.57–0.88, OR: 0.67, 95%CI: 0.51–0.86, and OR: 0.69, 95%CI: 0.55–0.86, respectively). Subgroup analyses showed that among all the different subgroups, high intake of flavanones was associated with lower odds of Ow/Ob and CO in children and adolescents.ConclusionA diet loaded with high flavanones were associated with lower odds of Ow/Ob and CO in children and adolescents, and children and adolescents should be encouraged to increase their intake of flavanones.

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Anti-diabetic effect of hesperidin on palmitate (PA)-treated HepG2 cells and high fat diet-induced obese mice

Cited by 9 publications

References 39 publications

Determination of Antioxidant, Anti-Alzheimer, Antidiabetic, Antiglaucoma and Antimicrobial Effects of Zivzik Pomegranate (Punica granatum)—A Chemical Profiling by LC-MS/MS

Determination of Antioxidant, Anti-Alzheimer, Antidiabetic, Antiglaucoma and Antimicrobial Effects of Zivzik Pomegranate (Punica granatum)—A Chemical Profiling by LC-MS/MS

Antiadipogenic Effect of Citrus Flavonoids: Evidence from RNA Sequencing Analysis and Activation of AMPK in 3T3-L1 Adipocytes

Association between intake of flavanones and the overweight/obesity and central obesity in children and adolescents: a cross-sectional study from the NHANES database

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