Anti-diabetic effect by walnut (Juglans mandshurica Maxim.)-derived peptide LPLLR through inhibiting α-glucosidase and α-amylase, and alleviating insulin resistance of hepatic HepG2 cells

Wang, Ji; Wu, Tong; Li, Fang; Liu, Chunlei; Liu, Xiaoting; Li, Hongmei; Shi, Junhua; Li, Meihe; Min, Weihong

doi:10.1016/j.jff.2020.103944

Cited by 99 publications

(81 citation statements)

References 53 publications

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“…Moreover, LL has the strongest effect in enhancing insulin signaling by promoting Ser473 phosphorylation of AKT, followed by VA. After the treatment of LL and VA, the gene expression levels of PEPCK and G6Pase in IR‐HepG2 cells were inhibited significantly so that glucose metabolism and glycogen synthesis process accelerated. Similar study was carried out by Wang (Wang et al., 2020) who isolated and purified pentapeptide LPLLR from walnut, and found that LPLLR (100 µM) increased glycogen synthesis and glucose uptake, as well as decreased gluconeogenesis via activating the IRS‐1/PI3K/Akt signal pathways thereby alleviating hepatic IR. GLUT2, a glucose transporter on hepatocyte membrane, is a signaling protein downstream of AKT.…”

Section: Discussionsupporting

confidence: 70%

Pea ‐derived peptides, VLP, LLP, VA, and LL, improve insulin resistance in HepG2 cells via activating IRS‐1/PI3K/AKT and blocking ROS‐mediated p38MAPK signaling

et al. 2020

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People recognized diabetes in 1500 BC (Litwack, 2018). However, so far, no reasonable treatment has been found (Zhang et al., 2019), so that diabetes has become a systemic chronic disease that threatens global human health (Xu, Li, Dai, & Peng, 2018). Diabetes includes type 1 diabetes (T1D) and type 2 diabetes (T2D), but the latter is more common accounting for 90~95% of all diabetics (Li et al., 2017) T1D is an autoimmune disease caused by insulin deficiency. However, in T2D, although insulin is still present, but its function is insufficient. When the effect of hormone is lacking, its target organs such as liver, muscle, and fat are inhibited in glucose utilization, which lead to insulin resistance (IR). IR is the main reason of T2D. (Fan et al., 2019). Liver is an insulin sensitive organ playing a key role in glucose metabolism (Meshkani & Adeli, 2009). Normally, there is a dynamic balance between gluconeogenesis and glycogen synthesis in the liver

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Section: Discussionsupporting

confidence: 70%

Pea ‐derived peptides, VLP, LLP, VA, and LL, improve insulin resistance in HepG2 cells via activating IRS‐1/PI3K/AKT and blocking ROS‐mediated p38MAPK signaling

et al. 2020

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“…This may indicate that these peptides may not fully inhibit glucose absorption but may help reduce oligosaccharide secretion. Peptides from food inhibiting α-amylase and α-glucosidase were demonstrated in previous studies [13,45,46]. Moreover, the different amino acid compositions and sequences probably affect the bioactivity of peptides.…”

Section: Discussionmentioning

confidence: 91%

“…Glucose absorption into the bloodstream in patients with insulin disorders causes a sharp increase in the blood glucose level, which may lead to development of the disease [11]. Peptides with enzyme inhibitory activity involved in polysaccharide metabolism and exhibiting a potent anti-diabetic effect were described previously: CSSV, YSPR, SAAP, PGGP, and LGGGNT [12], or LPLLR [13] Fats are energy-delivering molecules. Before being absorbed in the small intestine, they should be hydrolyzed by lipase into monoglycerol and free fatty acids.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of New Peptides GQLGEHGGAGMG, GEHGGAGMGGGQFQPV, EQGFLPGPEESGR, RLARAGLAQ, YGNPVGGVGH, and GNPVGGVGHGTTGT as Inhibitors of Enzymes Involved in Metabolic Syndrome and Antimicrobial Potential

et al. 2020

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The aim of this study was to determine the cytotoxic properties, influence on enzyme activity involved in metabolic syndrome, and antimicrobial activity of synthetic peptides with GQLGEHGGAGMG, GEHGGAGMGGGQFQPV, EQGFLPGPEESGR, RLARAGLAQ, YGNPVGGVGH, and GNPVGGVGHGTTGT sequences. Peptides have no cytotoxic effect on cells. The highest inhibitory effect on angiotensin converting enzyme I was noted for peptide GT-14 (IC50 = 525.63 µg/mL). None of the tested peptides had an influence on α-glucosidase. The highest α-amylase and lipase inhibitory activity was noted for GG-12 (IC50 = 56.72 and 60.62 µg/mL, respectively). The highest lipoxidase inhibitory activity was determined for peptide ER-13 (IC50 = 84.35 µg/mL). Peptide RQ-9 was characterized by the highest COX inhibitory activity (0.31 and 4.77 µg/mL for COX-1 and COX-2, respectively). Only peptide RQ-9 inhibited S. enteritidis ATCC 4931 growth (42–48%) in all tested concentrations (15.62–250 mg/mL).

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“…It should be noted that the LGGGN peptide showed higher inhibition of both α-amylase and α-glucosidase and can, thus, be considered as a potential antidiabetic inhibitor [ 55 ]. Wang et al [ 61 ] demonstrated that LPLLR inhibited both α-glucosidase and α-amylase, and the inhibition rates reached a maximum of 50.12% and 39.08%, respectively, at a concentration of 2000 μM. Peptides with α-amylase inhibitory activity were identified in basil seeds [ 62 ].…”

Section: Peptides As Inhibitors Of Enzymes Involved In Metabolic Smentioning

confidence: 99%

Current Trends of Bioactive Peptides—New Sources and Therapeutic Effect

Jakubczyk

Karaś

Rybczyńska‐Tkaczyk

et al. 2020

Foods

152

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Generally, bioactive peptides are natural compounds of food or part of protein that are inactive in the precursor molecule. However, they may be active after hydrolysis and can be transported to the active site. Biologically active peptides can also be synthesized chemically and characterized. Peptides have many properties, including antihypertensive, antioxidant, antimicrobial, anticoagulant, and chelating effects. They are also responsible for the taste of food or for the inhibition of enzymes involved in the development of diseases. The scientific literature has described many peptides with bioactive properties obtained from different sources. Information about the structure, origin, and properties of peptides can also be found in many databases. This review will describe peptides inhibiting the development of current diseases, peptides with antimicrobial properties, and new alternative sources of peptides based on the current knowledge and documentation of their bioactivity. All these issues are part of modern research on peptides and their use in current health or technological problems in food production.

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Anti-diabetic effect by walnut (Juglans mandshurica Maxim.)-derived peptide LPLLR through inhibiting α-glucosidase and α-amylase, and alleviating insulin resistance of hepatic HepG2 cells

Cited by 99 publications

References 53 publications

Pea ‐derived peptides, VLP, LLP, VA, and LL, improve insulin resistance in HepG2 cells via activating IRS‐1/PI3K/AKT and blocking ROS‐mediated p38MAPK signaling

Pea ‐derived peptides, VLP, LLP, VA, and LL, improve insulin resistance in HepG2 cells via activating IRS‐1/PI3K/AKT and blocking ROS‐mediated p38MAPK signaling

Characteristics of New Peptides GQLGEHGGAGMG, GEHGGAGMGGGQFQPV, EQGFLPGPEESGR, RLARAGLAQ, YGNPVGGVGH, and GNPVGGVGHGTTGT as Inhibitors of Enzymes Involved in Metabolic Syndrome and Antimicrobial Potential

Current Trends of Bioactive Peptides—New Sources and Therapeutic Effect

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