Characteristics of New Peptides GQLGEHGGAGMG, GEHGGAGMGGGQFQPV, EQGFLPGPEESGR, RLARAGLAQ, YGNPVGGVGH, and GNPVGGVGHGTTGT as Inhibitors of Enzymes Involved in Metabolic Syndrome and Antimicrobial Potential

Złotek, Urszula; Jakubczyk, Anna; Rybczyńska‐Tkaczyk, Kamila; Ćwiek, Paula; Baraniak, Barbara; Lewicki, Sławomir

doi:10.3390/molecules25112492

Cited by 20 publications

(17 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lipase activity was measured with p-nitrophenyl acetate (pNPA) as a substrate according to the method described by Wang et al [ 29 ] with modifications developed by Złotek et al [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

In vitro Antioxidant, Anti-inflammatory, Anti-metabolic Syndrome, Antimicrobial, and Anticancer Effect of Phenolic Acids Isolated from Fresh Lovage Leaves [Levisticum officinale Koch] Elicited with Jasmonic Acid and Yeast Extract

et al. 2020

Self Cite

View full text Add to dashboard Cite

Lovage seedlings were elicited with jasmonic acid (JA) and yeast extract (YE) to induce the synthesis of biologically active compounds. A simulated digestion process was carried out to determine the potential bioavailability of phenolic acids. Buffer extracts were prepared for comparison. The ability to neutralize ABTS radicals was higher in all samples after the in vitro digestion, compared to that in the buffer extracts. However, the elicitation resulted in a significant increase only in the value of the reduction power of the potentially bioavailable fraction of phenolic acids. The effect of the elicitation on the activity of the potentially bioavailable fraction of phenolic acids towards the enzymes involved in the pathogenesis of the metabolic syndrome, i.e., ACE, lipase, amylase, and glucosidase, was analyzed as well. The in vitro digestion caused a significant increase in the ability to inhibit the activity of these enzymes; moreover, the inhibitory activity against alpha-amylase was revealed only after the digestion process. The potential anti-inflammatory effect of the analyzed extracts was defined as the ability to inhibit key pro-inflammatory enzymes, i.e., lipoxygenase and cyclooxygenase 2. The buffer extracts from the YE-elicited lovage inhibited the LOX and COX-2 activity more effectively than the extracts from the control plants. A significant increase in the anti-inflammatory and antimicrobial properties was noted after the simulated digestion.

show abstract

“…Lipase activity was measured with p-nitrophenyl acetate (pNPA) as a substrate according to the method described by Wang et al [ 29 ] with modifications developed by Złotek et al [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

In vitro Antioxidant, Anti-inflammatory, Anti-metabolic Syndrome, Antimicrobial, and Anticancer Effect of Phenolic Acids Isolated from Fresh Lovage Leaves [Levisticum officinale Koch] Elicited with Jasmonic Acid and Yeast Extract

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…On the analysis day, 100 µL of PBS containing 2% FBS and 1 mM EDTA was added to the cells, and samples were centrifuged (500× g , 5 min). Ki67 staining was performed (according to the procedure recommended by the manufacturer), and PI staining followed the procedure described in [ 40 ]. Briefly, 50 µL of PBS containing 2% FBS, 1 mM EDTA, and 5 µL of Ki67-APC antibody was added to each sample and incubated for 30 min RT in the dark.…”

Section: Methodsmentioning

confidence: 99%

Effects of 445 nm, 520 nm, and 638 nm Laser Irradiation on the Dermal Cells

Szymański

Ciepielak

Cios

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

Background: The invention of non-ionizing emission devices revolutionized science, medicine, industry, and the military. Currently, different laser systems are commonly used, generating the potential threat of excessive radiation exposure, which can lead to adverse health effects. Skin is the organ most exposed to laser irradiation; therefore, this study aims to evaluate the effects of 445 nm, 520 nm, and 638 nm non-ionizing irradiation on keratinocytes and fibroblasts. Methods: Keratinocytes and fibroblasts were exposed to a different fluency of 445 nm, 520 nm, and 638 nm laser irradiation. In addition, viability, type of cell death, cell cycle distribution, and proliferation rates were investigated. Results: The 445 nm irradiation was cytotoxic to BJ-5ta (≥58.7 J/cm2) but not to Ker-CT cells. Exposure influenced the cell cycle distribution of Ker-CT (≥61.2 J/cm2) and BJ-5ta (≥27.6 J/cm2) cells, as well as the Bj-5ta proliferation rate (≥50.5 J/cm2). The 520 nm irradiation was cytotoxic to BJ-5ta (≥468.4 J/cm2) and Ker-CT (≥385.7 J/cm2) cells. Cell cycle distribution (≥27.6 J/cm2) of Ker-CT cells was also affected. The 638 nm irradiation was cytotoxic to BJ-5ta and Ker-CT cells (≥151.5 J/cm2). The proliferation rate and cell cycle distribution of BJ-5ta (≥192.9 J/cm2) and Ker-CT (13.8 and 41.3 J/cm2) cells were also affected. Conclusions: At high fluences, 455 nm, 520 nm, and 638 nm irradiation, representing blue, green, and red light spectra, are hazardous to keratinocytes and fibroblasts. However, laser irradiation may benefit the cells at low fluences by modulating the cell cycle and proliferation rate.

show abstract

“…Several AMPs have been identified so far that are able to show this activity, which depends on the structure and amino acid composition of the peptide ( Hüttl et al, 2013 ). CQPHPGQTC, EITPEKNPQLR and RKQEEDEDEEQQRE are three peptides from purified soybean β‐conglycinin that have been demonstrated to inhibit the pancreatic lipase ( Lunder et al, 2005 ; Martinez-Villaluenga et al, 2010 ), and are under investigation for potential clinical applications ( Złotek et al, 2020 ).…”

Section: Amps: Innate Weapons Against Diseasesmentioning

confidence: 99%

Antimicrobial Peptides: A New Hope in Biomedical and Pharmaceutical Fields

Moretta

Scieuzo

Petrone

et al. 2021

Front. Cell. Infect. Microbiol.

232

170

View full text Add to dashboard Cite

Antibiotics are essential drugs used to treat pathogenic bacteria, but their prolonged use contributes to the development and spread of drug-resistant microorganisms. Antibiotic resistance is a serious challenge and has led to the need for new alternative molecules less prone to bacterial resistance. Antimicrobial peptides (AMPs) have aroused great interest as potential next-generation antibiotics, since they are bioactive small proteins, naturally produced by all living organisms, and representing the first line of defense against fungi, viruses and bacteria. AMPs are commonly classified according to their sources, which are represented by microorganisms, plants and animals, as well as to their secondary structure, their biosynthesis and their mechanism of action. They find application in different fields such as agriculture, food industry and medicine, on which we focused our attention in this review. Particularly, we examined AMP potential applicability in wound healing, skin infections and metabolic syndrome, considering their ability to act as potential Angiotensin-Converting Enzyme I and pancreatic lipase inhibitory peptides as well as antioxidant peptides. Moreover, we argued about the pharmacokinetic and pharmacodynamic approaches to develop new antibiotics, the drug development strategies and the formulation approaches which need to be taken into account in developing clinically suitable AMP applications.

show abstract

Characteristics of New Peptides GQLGEHGGAGMG, GEHGGAGMGGGQFQPV, EQGFLPGPEESGR, RLARAGLAQ, YGNPVGGVGH, and GNPVGGVGHGTTGT as Inhibitors of Enzymes Involved in Metabolic Syndrome and Antimicrobial Potential

Cited by 20 publications

References 65 publications

In vitro Antioxidant, Anti-inflammatory, Anti-metabolic Syndrome, Antimicrobial, and Anticancer Effect of Phenolic Acids Isolated from Fresh Lovage Leaves [Levisticum officinale Koch] Elicited with Jasmonic Acid and Yeast Extract

In vitro Antioxidant, Anti-inflammatory, Anti-metabolic Syndrome, Antimicrobial, and Anticancer Effect of Phenolic Acids Isolated from Fresh Lovage Leaves [Levisticum officinale Koch] Elicited with Jasmonic Acid and Yeast Extract

Effects of 445 nm, 520 nm, and 638 nm Laser Irradiation on the Dermal Cells

Antimicrobial Peptides: A New Hope in Biomedical and Pharmaceutical Fields

Contact Info

Product

Resources

About