Anti-diabetic activity-guided screening of aqueous-ethanol Moringa oleifera extracts and fractions: Identification of marker compounds

Abstract

Purpose: To explore the anti-diabetic effects of Moringa oleifera extracts and fractions, and to identify their active/marker compounds. Methods: Five different aqueous ethanol extracts (95, 75, 50, 25 %v/v and 100 % water) (p < 0.01). No significant hypoglycemic activity was apparent, and the materials had no effect on IPGTT performance by normal rats. and

Moringa oleifera Lam. in Diabetes Mellitus: A Systematic Review and Meta-Analysis

“…Antioxidant activity can protect organs such as the pancreas, liver, and adipose tissues from hyperglycemia-mediated oxidative stress [38,51,[71][72][73]. This capacity is attributed to a high concentration of polyphenolics, including phenolic acids (gallic acid, chlorogenic acid, and caffeic acid) and flavonoids (quercetin, kaempferol, and myricetin) [44,64,74].…”

Moringa oleiferaand glycemic control: A review of current evidence and possible mechanisms

“…The most active extracts among them were further subjected into five liquid–liquid fractionations (hexane, chloroform, ethyl acetate, butanol, and water), and were evaluated for their antihyperglycemic activities. The results showed that 95% (v/v) ethanol extract at 1,000 mg/kg and butanol fraction at 500 mg/kg were considered the most active for reducing blood glucose concentration acutely in the diabetic rats (Irfan, Asmawi, Khan, Sadikun, & Mordi, ). In a similar study, Azad et al () measured fasting blood glucose and glucose tolerance after oral administration of ethanol extract of moringa leaves (500 mg/kg) or glibenclamide (0.5 mg/kg) as a reference drug or water (control), with a glucose solution (2.5 g/kg) in 12 hr fasted diabetic rats.…”

“…In another recent study, the bioactive compounds in moringa responsible for decreasing blood glucose concentration in diabetic rats were supposed to be cryptochlorogenic acid, quercetin 3‐ β ‐D‐glucoside and kaempferol‐3‐O‐glucoside, and these were identified through high‐performance liquid chromatography and thin‐layer chromatography. However, their mechanism of action was not determined (Irfan et al, ). Similarly, in moringa leaf extract administered rats, measurement with assay of six segments of gastrointestinal tract showed inhibition of sucrose digestion.…”

Efecto de las hojas de Moringa oleifera sobre el control de la glucemia en ratas diabéticas inducidas: revisión sistemática

“…Una vez confirmado la diabetes mellitus en el animal se esperó 72 horas para iniciar el tratamiento con el extracto de hojas de MO (177)(188)(182)(192)(39)(28)(179)(183) (137) .…”

“…En el extracto etanólico, también, se identificaron estos compuestos bioactivos como la presencia de fenoles, taninos, flavonoides, alcaloides, saponinas, terpenoides y glucósidos (179)(137) . El extracto acuoso carecía de alcaloides y terpenoides (137) . Las hojas de MO parecen tener una óptima calidad y cantidad de fenoles totales y flavonoides; estos compuestos combaten los síntomas de la diabetes y regeneran las células β del páncreas por sus efectos antioxidantes.…”